<![CDATA[Glioblastoma is the most aggressive primary malignant brain tumor and carries a poor prognosis. Current therapies are limited and often damage normal cells, so safer natural-based candidates are needed. Avocado seed (Persea americana Mill.) is an underused plant material rich in bioactive compounds with reported antioxidant and cytotoxic potential. This study aimed to analyze the effect of the aqueous fraction of avocado seed extract on the viability of T98G glioblastoma cells in vitro and to determine the most effective treatment concentration. The extract was obtained by maceration in 96% ethanol, followed by ethyl acetate–water fractionation to yield the aqueous fraction. Cells were treated with three concentrations (0.63, 1.25, and 2.50 mg/mL) for 24, 48, and 72 h. Cell viability was assessed using the MTT assay in three technical replicates, and the percentage of inhibition was analyzed descriptively. The cytotoxic effect was time-dependent. Mean inhibition increased from about 79% at 24 h to about 96% at 48 h, and to about 98% to 99% at 72 h. The highest inhibition was 98.79% at 1.25 mg/mL after 72 h. Increasing the concentration to 2.50 mg/mL did not raise inhibition further, and the differences among concentrations were small, so a clear dose-response relationship was not established. The aqueous fraction of avocado seed extract shows potential as a natural-based antiproliferative candidate against T98G glioblastoma cells. Further studies with biological replicates, inferential statistics, and phytochemical characterization are required to confirm its efficacy and mechanism.]]>
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