<![CDATA[Background: Oryza sativa L. is a major food commodity and a source of carbohydrates for most of Indonesia’s population. Based on BPS data, rice production has declined every year. The decline in rice production is caused by attacks by plant pests (OPT). Xanthomonas oryzae is a bacterium that causes bacterial leaf blight (BLB) in rice, which causes yield losses of up to 60-80%. An environmentally friendly alternative for controlling Xanthomonas oryzae is using endophytic bacteria Bacillus sp. and Pseudomonas fluorescens. Methods: The experiment was conducted using two tests: in vitro and in vivo assays. The in vitro assay was arranged in a completely randomized design with 4 treatments and 4 replications, resulting in 16 experimental units. The experimental unit consisted of one Petri dish containing Nutrient Agar (NA) medium. Antagonistic activity was evaluated using the disk diffusion method against Xanthomonas oryzae. The in vivo assay was arranged in a completely randomized factorial design with 10 treatments and 3 replications. Each replication consisted of 6 rice plants grown in a polybag, resulting in a total of 180 plants as experimental units. Treatments consisted of different concentrations of antagonistic bacterial suspensions applied to soil before pathogen inoculation. The pathogen Xanthomonas oryzae was inoculated onto rice plants during the vegetative stage. Results: Bacillus sp. and Pseudomonas fluorescens can suppress Xanthomonas oryzae disease. The results showed that a consortium treatment of these two bacteria produced the highest inhibitory power of 9,125 mm. In vivo testing, the consortium treatment with 20 ml/plant resulted in the lowest disease intensity of 6,06% and an incubation period of 4 days. Conclusions: The consortium treatment of Bacillus sp. and Pseudomonas fluorescens was able to suppress bacterial leaf blight disease compared to other treatments.]]>
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