BIO-EDU: Jurnal Pendidikan Biologi
Vol. 11 No. 2 (2026): BIOEDU: Jurnal Pendidikan Biologi - August 2026

Detection of Parasitic Contamination in Nile Tilapia (Oreochromis niloticus) and Fresh Spinach (Amaranthus hybridus) from Pramuka Market, Samarinda

Tria Lestari Agus Salim (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, 75119, East Kalimantan, Indonesia)
Febry Rahmadhani Hasibuan (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, 75119, East Kalimantan, Indonesia)
Hasbiah (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, 75119, East Kalimantan, Indonesia)
Nova Hariani (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, 75119, East Kalimantan, Indonesia)
Muhammad Fahril Azhar (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, 75119, East Kalimantan, Indonesia)
Irene Helena Sitorus (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, 75119, East Kalimantan, Indonesia)
Erina Yulistin Sianipar (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, 75119, East Kalimantan, Indonesia)
Jia Rahmawati (Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Mulawarman, Samarinda, 75119, East Kalimantan, Indonesia)



Article Info

Publish Date
30 Aug 2026

Abstract

This preliminary study aimed to detect parasitic contamination in Nile tilapia (Oreochromis niloticus) and fresh spinach (Amaranthus hybridus) sold at Pramuka Market, Samarinda, as part of an effort to assess biological food safety at the local market level. A descriptive observational design was employed. Samples comprised three Nile tilapia and 200 grams of spinach obtained from Pramuka Market, Samarinda. Ectoparasite examination in fish was conducted using the mucus-scraping method on the body surface and gills, while parasite egg examination in spinach was performed using the simple flotation method. Parasite identification was based on morphological characteristics observed under a light microscope. Results of the three Nile tilapia examined, one fish (33.3%) was found to harbor the ectoparasite Trichodina sp. on the gill mucus. In the 200‑gram spinach sample, a single egg presumed to be Fasciola sp. was detected, identified by its oval shape, yellowish‑brown color, and the presence of an operculum. This study detected indications of parasitic contamination in both fish and spinach samples from Pramuka Market, Samarinda, suggesting a potential biological risk in fresh food commodities sold at the market. However, due to the limited sample size and the absence of molecular confirmation, these results should be interpreted as indicative rather than conclusive and cannot be generalized. Further studies with larger sample sizes and molecular confirmation (e.g., polymerase chain reaction) are recommended to support more comprehensive food safety surveillance.

Copyrights © 2026