<![CDATA[The presence of airborne microorganisms in laboratory environments may increase the risk of microbial exposure among laboratory personnel. Indoor bioaerosols can affect air quality and potentially pose health risks if environmental conditions are not adequately controlled. This study aimed to identify airborne bacteria and fungi present in the office room of the Microbiology Laboratory, Faculty of Medicine, Universitas Jenderal Achmad Yani. Air sampling was performed using the passive settle plate method at three sampling points, with Tryptic Soy Agar (TSA) used for bacterial isolation and Sabouraud Dextrose Agar (SDA) used for fungal isolation. Environmental measurements during sampling recorded a temperature of 25.1°C and a relative humidity of 63%. Bacterial and fungal growth were observed on TSA and SDA plates, respectively, with bacterial colony counts ranging from 0 to 24 colonies. A total of six bacterial isolates were successfully recovered. Bacterial identification was performed based on colony morphology observation, Gram staining, biochemical tests, and molecular analysis using polymerase chain reaction (PCR) and DNA sequencing. Fungal identification was conducted based on macroscopic characteristics and microscopic examination using Lactophenol Cotton Blue staining. Molecular analysis identified the bacterial isolates as Staphylococcus hominis, Staphylococcus epidermidis, Mammaliicoccus sciuri, and Stutzerimonas stutzeri, whereas fungal isolates were morphologically consistent with Penicillium sp. and other suspected hyaline filamentous fungi. The predominance of Gram-positive bacteria and the detection of airborne fungi may be associated with limited ventilation, indoor humidity, and human-related activities within the laboratory environment. 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