Healthcare-Associated Infections(HAIs) remains a patient safety issue that can be prevented through proper hand hygiene implementation. The audit results at Ja'far Medika Karanganyar General Hospital from October to December 2025 showed that hand hygiene accuracy was 77.5%, 77.7%, and 78.9%, respectively, which are still below the optimal standard of ≥85%. This study aims to determine the accuracy of hand hygiene implementation among nurses and the characteristics of nurses based on age, gender, education, and length of service. The study used a quantitative approach with a descriptive observational design. The population and sample consisted of all 49 nurses at Ja'far Medika Karanganyar General Hospital selected using total sampling. Data were collected through direct observation using an observation sheet based on the hospital's handrub SOP. The instrument was validated through expert judgment using I-CVI and Aiken's V. Data were analyzed univariately using SPSS and presented in frequencies and percentages. The results showed that 28 nurses (57.1%) were in the good category, 8 (16.4%) were in the sufficient category, and 13 (26.5%) were in the poor category. The highest accuracy was found in the five moments (100%), while the lowest was found in fingertip/nail movements (63.2%), thumb (69.3%), and back of finger (75.5%). In conclusion, hand hygiene accuracy was generally good, but technique in some areas of the hand still needs improvement
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