Journal of Biomedical Sciences and Health
Vol. 3 No. 2 (2026)

Formulation and characterization of gotu kola (Centella asiatica) and cinnamon (Cinnamomum verum)-enriched white bread: organoleptic properties and qualitative triterpenoid screening

Lisa Istarofah (Department of Nutrition, Faculty of Health Sciences, Universitas Ivet, Semarang, Indonesia)
Umar Hidayat (Department of Nutrition, Faculty of Health Sciences, Universitas Ivet, Semarang, Indonesia)
Chairunisa Nur Rarastiti (Department of Nutrition, Faculty of Health Sciences, Universitas Ivet, Semarang, Indonesia)



Article Info

Publish Date
23 Aug 2026

Abstract

Background: Elevated blood pressure and stage 1 hypertension, as classified by the 2017 ACC/AHA guideline, increase cardiovascular risk substantially. Non-pharmacological dietary interventions incorporating bioactive plant-derived compounds into staple foods represent a promising preventive strategy. Methods: A completely randomized design with four treatment groups (F0, F1, F2, F3) and three replications per group was employed. White bread was formulated with gotu kola (Centella asiatica) leaf powder and cinnamon (Cinnamomum verum) bark powder at varying proportions: F0 (0%:0%), F1 (10%:20%), F2 (20%:10%), and F3 (15%:15%). Organoleptic evaluation was conducted by 25 semi-trained panelists using a two-point hedonic scale. Data were analyzed using the Kruskal-Wallis H test with Dunn’s post hoc comparison. Triterpenoid content was identified through qualitative phytochemical screening. Results: The Kruskal-Wallis test revealed statistically significant differences among formulations for all four sensory attributes: taste (H(3) = 29.28, p < 0.001), texture (H(3) = 19.51, p < 0.001), aroma (H(3) = 15.80, p = 0.001), and color (H(3) = 11.68, p = 0.009). Post-hoc analysis indicated that the control (F0) was significantly preferred over all treatment formulations across most attributes. Among treatment formulations, F2 demonstrated the highest taste acceptance (52%). Qualitative phytochemical analysis confirmed the presence of triterpenoid compounds exclusively in F2. Conclusion: The F2 formulation (20% gotu kola, 10% cinnamon) was identified as the preferred formulation based on combined sensory performance and qualitative triterpenoid detection. Further quantitative analysis, bioavailability assessment, and controlled clinical investigation are warranted before functional health claims can be established.

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Journal Info

Abbrev

jbsh

Publisher

Subject

Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health

Description

The Journal of Biomedical Sciences and Health (JBSH) accepts manuscripts in basic sciences and applied clinical research. It covers various fields of biomedical sciences, but is not limited to Medical sciences. Our journal accepts and publishes original articles and review articles that ...