<![CDATA[Shallots (Allium cepa L.) are among the most important vegetable crops commonly cultivated in Indonesia. Anthracnose disease, caused by Colletotrichum sp., is a major constraint in shallot production, causing yield losses of up to 50% or more. Sustainable approaches for suppressing anthracnose disease using plant-based fungicides developed with nanotechnology seem promising. This study aimed to evaluate and determine the effective concentration of Anredera cordifolia (binahong) leaf nanoextract combined with chitosan nanoparticles (chitosan NPs) in controlling the in vitro growth of Colletotrichum sp. and reducing anthracnose disease on shallot plants. Chitosan nanoparticles and binahong leaf nanoextract were evaluated individually and in combination under in vitro and greenhouse conditions, including comparisons with acetic acid and mancozeb treatments. The results indicated that the application of chitosan NPs, both alone and in combination with binahong leaf nanoextract, was effective in suppressing the in vitro growth of Colletotrichum sp. The treatment consisting of 500 ppm chitosan NPs combined with 250 ppm binahong leaf nanoextract resulted in a 66% reduction of anthracnose disease on shallots, which was comparable to the mancozeb application.]]>
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