Mathematical literacy is an essential 21st-century competency that enables students to formulate, use, and interpret mathematical concepts in various real-life contexts. However, Indonesian students’ mathematical literacy remains relatively low, highlighting the need for innovative learning media to support its development effectively. This study aimed to develop and evaluate an ethnomathematics-based e-comic integrating the cultural context of the Cheng Hoo Mosque in Jember to enhance students’ mathematical literacy. This Research and Development (R&D) study employed the ADDIE model, consisting of the Analysis, Design, Development, Implementation, and Evaluation stages. The participants included expert validators, teachers, and eighth-grade students of Al-Falah Silo Junior High School, Jember, Indonesia. Data were collected through expert validation sheets, teacher and student response questionnaires, and mathematical literacy tests. The developed e-comic achieved material and media validation scores of 95.40% and 94.45%, respectively, indicating high validity. Student and teacher responses reached 95.21% and 91.67%, respectively, demonstrating high practicality. Furthermore, students’ mathematical literacy improved significantly, as indicated by the results of an independent-samples t-test (p < 0.001) and a high N-gain score of 0.72. These findings indicate that the ethnomathematics-based e-comic is a valid, practical, and effective learning medium for enhancing students’ mathematical literacy.
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