<![CDATA[Cassia alata is a medicinal plant widely used as a traditional laxative. Despite its therapeutic benefits, prolonged use may induce toxic effects, necessitating safety evaluation through subchronic toxicity studies. This study aimed to assess the effects of ethanolic C. alata leaf extract on renal histopathology in white rats (Rattus norvegicus). Thirty-six male and female Sprague-Dawley rats were allocated into six groups: negative control (NC), extract-treated groups receiving 100 (D1), 300 (D2), and 900 mg/kg BW (D3), satellite negative control (SNC), and high-dose satellite (SD3). The extract was administered orally for 28 days. Kidney tissues were collected and examined histopathologically using hematoxylin-eosin staining. Evaluated lesions included glomerular alterations (atrophy and edema) and tubular changes consisting of hydropic degeneration, proteinaceous casts, and necrosis. Statistical analysis revealed no significant differences in glomerular lesions among treatment groups (p>0.05). In contrast, cortical tubular hydropic degeneration and necrosis increased after extract treatment (p<0.05), particularly at the highest dose. No significant differences were observed between the 100 mg/kg BW group and the control group. These findings indicate that ethanolic C. alata leaf extract is relatively safe at 100 mg/kg BW under subchronic conditions but may induce dose-dependent renal tubular histopathological alterations at higher doses.]]>
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