<![CDATA[Diabetic wounds remain a major clinical challenge, as persistent hyperglycemia disrupts fibroblast function and matrix remodeling, delaying healing. Transforming Growth Factor-beta 1 (TGF-beta1) is a key regulator of fibroblast proliferation and collagen synthesis during repair. This pilot study explored Moringa oleifera gel's wound-healing effects using an integrated in vivo in silico approach, generating hypotheses about its mechanism via the TGF-beta1 pathway. Twenty-five streptozotocin-induced diabetic Wistar rats with partial-thickness (Grade II) burn wounds were randomized to 0.5% Na-CMC (negative control), Bioplacenton (positive control), or M. oleifera gel (5%, 10%, or 15%) once daily for 14 days. Because histomorphometric analysis used only two animals per group (n = 2/group), fibroblast and collagen outcomes are reported descriptively as preliminary, pilot-stage observations. The 15% gel group showed descriptively higher mean fibroblast counts than the negative control (110.0 pm 9.9 vs. 54.5 pm 4.9 cells/3 HPF) and higher collagen coverage (score 3, about 80%) than untreated wounds (score 2, about 20%). Molecular docking showed quercetin, a major M. oleifera constituent, has favorable predicted affinity (-9.6 kcal/mol) toward TGF-betaR1's ATP-binding pocket; this is hypothesis-generating only. These preliminary findings suggest topical M. oleifera gel may warrant further investigation as a candidate adjunctive therapy for diabetic wound management.]]>
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