<![CDATA[Irrational antibiotic use remains a global public health problem that contributes to the increasing prevalence of antibiotic resistance. Adolescents' lack of knowledge regarding antibiotic use is one of the factors contributing to antibiotic misuse. This study aimed to determine the effect of educational interventions using video and leaflet media on improving adolescents' knowledge and to compare the effectiveness of the two educational media. This study employed a quantitative method with a quasi-experimental pre-test–post-test two-group design. The sample consisted of 114 twelfth-grade students at Senior High School Y, Merangin Regency, selected using a purposive sampling technique and divided into a video group and a leaflet group. Knowledge levels were measured using a questionnaire administered before and after the intervention. Data were analyzed using the Wilcoxon Signed Rank Test and the Mann–Whitney test. The results showed that educational interventions using both video and leaflet media significantly improved adolescents' knowledge (p < 0.001). The mean knowledge score in the video group increased from 76.30 to 94.39, while the mean score in the leaflet group increased from 70.28 to 90.93. The Mann–Whitney test indicated a significant difference in effectiveness between the two media (p < 0.05), with the video group achieving a higher mean score. This study concludes that both video and leaflet media are effective in improving adolescents' knowledge of the rational use of antibiotics.]]>
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