<![CDATA[Endodontic infections involving Treponema denticola and Aggregatibacter actinomycetemcomitans are highly resistant due to biofilm formation, requiring an effective and biocompatible irrigant alternative. This study aimed to evaluate the antibacterial and antibiofilm activities of Moringa oleifera leaf extract against these bacteria using in vitro and in silico approaches. The dilution method was used to determine the minimum inhibitory concentration, biofilm assays were performed using crystal violet staining, and molecular docking was conducted to predict interactions between moringa leaf bioactive compounds and bacterial target proteins. Results showed that an 80% concentration of M. oleifera extract demonstrated the highest antibacterial and antibiofilm activities, significantly reducing bacterial colonies and optical density (OD) values, with effectiveness comparable to 5.25% sodium hypochlorite. In silico analysis confirmed strong binding affinity and favorable ADMET properties. It is concluded that M. oleifera extract has potential as a natural alternative root canal irrigant to inhibit bacterial growth and disrupt biofilm. Further in vivo studies are recommended to evaluate clinical safety and long-term effectiveness.]]>
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