Journal Health Applied Science And Technology (JHAST)
Vol 4 No 2 (2026): Journal Health Applied Science And Technology (JHAST)

The Effectiveness of Neutral Buffered Formalin in Preserving Urine Erithrocytes with Delayed Testing

Mardyana Nizar (Poltekkes Kemenkes Palembang)
Trisna Citra (Poltekkes Kemenkes Banten)



Article Info

Publish Date
30 Sep 2026

Abstract

Urine erythrocyte examination is one of the important parameters in urinalysis to detect hematuria and abnormalities of the urinary system. Delays in examination often become a challenge in laboratories, as erythrocytes are prone to lysis or morphological changes that may affect result accuracy. This study aimed to determine the effectiveness of Neutral Buffered Formalin (NBF) as a fixative in maintaining the count and morphology of urine erythrocytes at different examination delay times: 0 hours as control, and delays of 3 hours and 6 hours. Urine samples were collected using purposive sampling, and erythrocyte sediment examination was performed using a light microscope. Data analysis employed the Dependent Sample T-Test to compare results across each delay interval. The findings showed that the use of NBF was able to maintain the stability of urine erythrocytes up to a 6-hour delay, with differences in count and morphology that were not statistically significant (p > 0.05). These results indicate that NBF is effective as a fixative to preserve the quality of urine erythrocyte examination despite delays in analysis.

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Journal Info

Abbrev

jhast

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Education Environmental Science Health Professions Immunology & microbiology Public Health

Description

JHAST adalah kepanjangan dari Journal Health Applied Science And Technology, JHAST merupakan jurnal fakultas Sains dan Teknologi Ilmu Kesehatan dan Teknologi Muhammadiyah Palembang. Fakultas Sains dan Teknologi terdiri dari Program studi : 1. Prodi S1 GIZI 2. Prodi S1 Sistem Informasi 3. Prodi DIV ...