Effect of chicken feet collagen ointment (Gallus gallus domesticus L.) on incision wound healing in mice (Mus musculus L.)

Zulfa Hafidzah (Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman)
Rudy Agung Nugroho (Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman)
Retno Aryani (Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman)
Reni Kurniati (Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman)
Eko Kusumawati (Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mulawarman)

Bioeksperimen
Vol. 12 No. 2: September 2026

Chicken feet (Gallus gallus domesticus L.) are a by-product of a chicken slaughterhouse that has the potential to be a source of collagen for wound healing therapy. Collagen plays an important role in skin tissue regeneration, protein synthesis, and connective tissue formation during the healing process. This study aimed to characterize chicken feet collagen using Fourier Transform Infrared (FTIR), evaluate the physicochemical properties of collagen ointment preparations, and analyze their effect on the percentage of incision wound closure, protein levels, and hydroxyproline in mice (Mus musculus L.). The study was conducted as a laboratory experiment with a complete randomized design, including negative controls, two positive controls, and three groups of collagen ointment at concentrations of 5%, 10%, and 15%. Collagen is extracted using the Acid Soluble Collagen (ASC) method and is formulated in the form of an ointment. FTIR characterization shows the presence of amide groups A, B, I, II, and III as characteristic of collagen. The ointment preparation meets physical and chemical requirements, including homogeneity, semi-solid consistency, and pH according to the skin. Collagen ointment increased the percentage of incision wound closure across all groups without a significant difference among treatments. Tissue protein levels declined progressively from the untreated control through the treated groups, reaching the lowest value at the 5% concentration (P1). Hydroxyproline levels, by contrast, did not follow a uniform pattern: the 5% ointment (P1) showed hydroxyproline levels comparable to the positive controls, whereas the 10% and 15% ointments (P2, P3) showed hydroxyproline levels comparable to the untreated negative control, suggesting that healing in the P2 and P3 groups remained in an active, collagen-synthesizing phase at the time of sampling. These results suggest that chicken feet collagen has the potential to be an active ingredient in topical preparations to support the healing of incision wounds.