Effects of Reduction of MHCII Expression on M1 Polarity Activated HMC3 Microglia Cells By n-Hexane And Ethyl Acetate Fraction of Semanggi (Marsilea crenata Presl.) Leaves: Efek Penurunan Ekspresi MHCII Pada Sel Mikroglia HMC3 Teraktivasi M1 Polarity Oleh Fraksi n-Heksana Dan Etil Asetat Daun Semanggi (Marsilea crenata Presl.)

Burhan Ma'arif (Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang)
Faisal Akhmal Muslikh (Mahasiswa Magister Ilmu Farmasi, Universitas Airlangga, Surabaya)
Abdul Malik Guhir (Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang)
Hilwa Fitri (Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang)
Luqman Alfani Najib (Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang)
Novyananda Salmasfattah (Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang)
Hening Laswati (Departemen Ilmu Kedokteran Fisik dan Rehabilitasi, Fakultas Kedokteran, Universitas Airlangga, Surabaya)
Mangestuti Agil (Departemen Ilmu Farmasi, Fakultas Farmasi, Universitas Airlangga, Surabaya)

Journal Pharmasci (Journal of Pharmacy and Science)
Vol. 7 No. 1 (2022): Journal Pharmasci (Journal of Pharmacy and Science)

Neuroinflammatory is a pathological condition in which neuronal cells experience progressive loss of function, one of which is due to estrogen deficiency. Phytoestrogens are compound that has structure and function similar to 17β-estradiol, the most potent natural form of estrogen. Semanggi (Marsilea crenata Presl.) is known to contain phytoestrogens which can replace the function of 17β-estradiol in maintaining organ homeostasis, so that is potential to be developed as an anti-neuroinflammatory drug. This study was aimed to prove the anti-neuroinflammatory effect of the n-hexane and ethyl acetate fraction of Semanggi leaves on HMC3 microglia cells, by measuring the expression of major histocompatibility complex II (MHC II). Both fractions with a concentration of 62.5; 125; and 250 μg/ml were administered to HMC3 microglia cells which had been induced by interferon gamma (IFN-γ) for 24 hours. Measurement of MHC II expression were using immunocytochemistry (ICC) methods and confocal laser scanning microscopy (CLSM) instruments. The results showed that n-hexane fraction could reduce MHC II expression at concentrations of 250 μg/ml with p<0.05, and showed a non-monotonic dose response (NMDR) pattern, while ethyl acetate fraction could reduce MHC II expression at all concentrations with p<0.05. Based on the results of the correlation test, the best anti-neuroinflammatory activity on HMC3 microglia cells is showed by the ethyl acetate fraction of Semanggi leaves.