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All Journal Journal Pharmasci (Journal of Pharmacy and Science)
Novyananda Salmasfattah
Program Studi Farmasi, Fakultas Kedokteran dan Ilmu Kesehatan, Universitas Islam Negeri Maulana Malik Ibrahim, Malang
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology

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Effects of Reduction of MHCII Expression on M1 Polarity Activated HMC3 Microglia Cells By n-Hexane And Ethyl Acetate Fraction of Semanggi (Marsilea crenata Presl.) Leaves: Efek Penurunan Ekspresi MHCII Pada Sel Mikroglia HMC3 Teraktivasi M1 Polarity Oleh Fraksi n-Heksana Dan Etil Asetat Daun Semanggi (Marsilea crenata Presl.) Burhan Ma'arif; Faisal Akhmal Muslikh; Abdul Malik Guhir; Hilwa Fitri; Luqman Alfani Najib; Novyananda Salmasfattah; Hening Laswati; Mangestuti Agil
Journal Pharmasci (Journal of Pharmacy and Science) Vol. 7 No. 1 (2022): Journal Pharmasci (Journal of Pharmacy and Science)
Publisher : Akademi Farmasi Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53342/pharmasci.v7i1.271

Abstract

Neuroinflammatory is a pathological condition in which neuronal cells experience progressive loss of function, one of which is due to estrogen deficiency. Phytoestrogens are compound that has structure and function similar to 17β-estradiol, the most potent natural form of estrogen. Semanggi (Marsilea crenata Presl.) is known to contain phytoestrogens which can replace the function of 17β-estradiol in maintaining organ homeostasis, so that is potential to be developed as an anti-neuroinflammatory drug. This study was aimed to prove the anti-neuroinflammatory effect of the n-hexane and ethyl acetate fraction of Semanggi leaves on HMC3 microglia cells, by measuring the expression of major histocompatibility complex II (MHC II). Both fractions with a concentration of 62.5; 125; and 250 μg/ml were administered to HMC3 microglia cells which had been induced by interferon gamma (IFN-γ) for 24 hours. Measurement of MHC II expression were using immunocytochemistry (ICC) methods and confocal laser scanning microscopy (CLSM) instruments. The results showed that n-hexane fraction could reduce MHC II expression at concentrations of 250 μg/ml with p<0.05, and showed a non-monotonic dose response (NMDR) pattern, while ethyl acetate fraction could reduce MHC II expression at all concentrations with p<0.05. Based on the results of the correlation test, the best anti-neuroinflammatory activity on HMC3 microglia cells is showed by the ethyl acetate fraction of Semanggi leaves.
Co-Authors Abdul Malik Guhir Burhan Ma&#039;arif Faisal Akhmal Muslikh Hening Laswati Putra Hilwa Fitri Luqman Alfani Najib Mangestuti Agil