Journal of Food and Pharmaceutical Science
Vol 14, No 5 (2026): J.Food.Pharm.Sci

The Effect of Total Flavonoid Content of Butterfly Pea Flower Kombucha on Reducing Serum Total Cholesterol Levels in vitro and in vivo in Rats

Agus Suprijono (Semarang Pharmacy Foundation College of Pharmaceutical Science, Semarang, Central Java, Indonesia)
Aries Koes Sundoro (Semarang Pharmacy Foundation College of Pharmaceutical Science, Semarang, Central Java, Indonesia)
Ariani Hesti Wulan S (Semarang Pharmacy Foundation College of Pharmaceutical Science, Semarang, Central Java, Indonesia)



Article Info

Publish Date
26 Oct 2026

Abstract

High-cholesterol foods cause metabolic disorders characterized by high cholesterol levels. Butterfly Pea Flower Kombucha, which contains anthocyanins (flavonoids), was obtained by fermenting a mixture of fresh butterfly pea flowers with a SCOBY (Symbiotic culture of bacteria and yeast). This study aimed to determine the effect of butterfly pea flower kombucha in reducing total cholesterol. The study began with the production of butterfly pea flower kombucha from 30%, 40%, and 50% fresh butterfly pea flowers fermented with a SCOBY. Total flavonoid levels were determined using visible spectrophotometry with a wavelength of 439.00 nm. Cholesterol levels were determined in vivo by feeding fermented tea to groups of white rats (C-, C+, S1, S2, and S3) for 10 days, after which they were fed lard for 14 days. The results showed that butterfly pea flower kombucha of 30%, 40%, and 50% had a total flavonoid content of 55.75 + 0.656, 84.59 + 1.912, and 154.38 + 0.476 mgQE/100mL. The sample and control could reduce cholesterol by an average of 11.83 + 0.94%, 21.14 + 1.58%, 31.36 + 0.98%, and 32.85 + 1.17%. Statistical tests showed the effect of butterfly pea flower kombucha which can lower cholesterol. This study shows that consuming butterfly pea flower kombucha can reduce cholesterol.

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Journal Info

Abbrev

jfps

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Chemistry Immunology & microbiology Medicine & Pharmacology

Description

FOCUS AND SCOPE Journal of Food and Pharmaceutical Sciences offers scientists, researchers, and other professionals to share knowledge of scientific advancements. The journal will publish original research articles, review articles, short communication, and letter to editor. The area of focus should ...