<![CDATA[Fixation is a crucial step in preparing histological specimens and has a significant impact on the quality of the tissue to be examined under a microscope. The use of Neutral Buffer Formalin (NBF) at inappropriate concentrations or for improper fixation times can affect the quality of Hematoxylin and Eosin (HE) staining results. This study aims to determine the effect of variations in NBF concentration and fixation time on the intensity and quality of HE staining results using mouse colon tissue (Mus musculus). This study is a quasi-experimental study with a descriptive quantitative approach. The study samples consisted of mouse colon tissue subjected to four fixation treatments at room temperature: 5% NBF for 48 hours, 10% NBF for 24 hours, 15% NBF for 16 hours, and 20% NBF for 12 hours. The results of this study were scores analyzed using the Shapiro-Wilk test, and a significance value of 0.004 (≤0.05) was obtained, indicating that the research data were not normally distributed; the analysis was then continued with the Kruskal-Wallis test. Based on the results of the Kruskal-Wallis test, an Asymp. Sig. value of 1.000 (≥0.05) was obtained, indicating no significant difference in histological staining quality among the variations in concentration and fixation time of Neutral Buffer Formalin.]]>
Copyrights © 2026
