Biology, Medicine, & Natural Product Chemistry
Vol 15, No 2 (2026)

Formula Optimization and Physicochemical Characteristics of Functional Instant Beverage from Beetroot (Beta vulgaris L.) and Cinnamon (Cinnamomum spp.)

Ainul Haqqi Robbaniyyah (Brawijaya University)
Widya Dwi Rukmi Putri (Brawijaya University)
Endrika Widyastuti (Brawijaya University)
Inges Manggar Gading (Brawijaya University)



Article Info

Publish Date
03 Nov 2026

Abstract

Instant beverages are food products in the form of fine, dry granules with low moisture content, typically made from fruits, vegetables, and grains. This study aims to determine the optimal ratio of beet extract, cinnamon extract, and maltodextrin in a functional instant beverage and to evaluate its physicochemical characteristics. The extracts were processed via foam mat drying at 60°C, and the resulting dried sheets were ground into powder. Formulation optimization was performed using the Response Surface Method with a D-Optimal Mixing Design in Design Expert 13 software, considering beet extract, cinnamon extract, and maltodextrin as factors, and betalain content, nitrate content, and solubility as response variables. The optimal formulation obtained was 75% beet extract, 15% cinnamon extract, and 10% maltodextrin, with predicted values of 46.99 mg/100 g for betalain content, 2060.84 mg/kg for nitrate content, and 94.58% for solubility. Verification results showed a betalain content of 44.76 mg/100 g, a nitrate content of 2082.12 mg/kg, and a solubility of 94.68%, with a desirability value of 0.96, indicating no significant difference between the predicted and actual values. Physicochemical characterization of the optimal formula revealed a water solubility index (WSI) of 95.40%, color values of L = 42.37, a = 16.79, and b = 5.33, a moisture content of 4.30%, and a half-maximal inhibitory concentration (IC50) of 943.74 mg/L. These results indicate that the optimal formulation produces an instant beverage that maintains stable bioactive compounds and exhibits good solubility, making it a promising candidate for a functional beverage.

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Journal Info

Abbrev

BIOMEDICH

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Public Health

Description

BIOLOGY, MEDICINE, & NATURAL PRODUCT CHEMISTRY, this journal is published to attract and disseminate innovative and expert findings in the fields of plant, animal, and microorganism secondary metabolite, and also the effect of natural product on biological system as a reference source for ...