<![CDATA[Adolescent athletes require optimal physical fitness to perform at a high level. However, adolescents' physical fitness in Indonesia remains low, with an average VO₂max of 36 ml/kg/min. Low hemoglobin levels can influence VO₂max because hemoglobin plays a role in oxygen binding and may increase fatigue in athletes. This study aimed to determine the relationship between hemoglobin (Hb) levels and physical fitness among adolescent football athletes. This observational analytic study used a cross-sectional design and involved 31 adolescent football athletes aged 13–17 years in June-July 2023 at the PSS Sleman Development Center. The sampling technique used purposive sampling. Hemoglobin levels were obtained through venous blood sampling and analyzed in a laboratory using the Cyanmethemoglobin method. Physical fitness data were obtained using the Multistage Fitness Test and presented by score. Data were analyzed using the Pearson Product-Moment test with p<0.05. The results showed that the average hemoglobin level and physical fitness score were 14.80±0.881 g/dL and 48.88±3.36 ml/kg/min, respectively. All athletes had normal hemoglobin levels (100%) and excellent physical fitness (100%). There was no relationship between hemoglobin levels and physical fitness among adolescent football athletes (p=0.4812, r=-0.1314). Athletes need to maintain hemoglobin levels and physical fitness to improve performance.]]>
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