Background: Tooth discoloration is a common aesthetic problem and can affect self-confidence. One solution to this problem is a tooth whitening procedure, using either chemical or natural ingredients. Objective: To compare the effectiveness of strawberry juice and blueberry juice on tooth discoloration during the teeth whitening process. Methods: This was a laboratory experimental study using a pre-test and post-test control group design. Twenty-four permanent first and second premolar teeth were immersed in red grape extract and green grape extract for 1, 3, and 5 hours, respectively. Tooth color was measured before and after immersion using a VITA Easyshade V digital spectrophotometer, based on L (lightness), C (chroma), and H (hue) values. Results: The study showed an increase in L values and a decrease in C and H values, indicating a significant difference in tooth color (sig <0.05) across all immersion groups, using both strawberry juice and blueberry juice. The most significant increase in L values occurred in the 5-hour soaking group with strawberry juice. Conclusion: There is a difference in effectiveness between strawberry juice and blueberry juice in the tooth whitening process. Strawberry juice is more effective as a natural external tooth whitening agent, especially with longer soaking times, due to its active ingredients such as malic acid, electic acid, vitamin C, and phenolic compounds. There is also a difference in pH between strawberry and blueberry juices. The pH value of strawberry juice is lower than that of blueberry juice
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