Background: The lack of local data on the success of Artificial Insemination (AI) in Aceh cattle is an obstacle in planning population increase programs. This study, therefore, is very urgent to fill the information gap and provide a scientific basis for improving local livestock reproduction strategies. Evaluation of Conception Rate (CR) and Service per Conception (S/C) in Aceh cattle in Seruway District will assess the effectiveness of AI, identify obstacles and opportunities, and provide references for the Livestock Service Office in designing targeted programs. Purpose: The objective of this study was to determine the CR and S/C of Artificial Insemination of Aceh Cattle in Seruway District, Aceh Tamiang Regency. Methods: The method used is descriptive quantitative. The data used consist of primary and secondary data. Primary data were obtained through semi-structured interviews, field observations, and recording of AI results, while secondary data came from reports and reproductive records of the local Livestock Service. The research sample was determined purposively, involving 156 breeders (369 female cattle that were AI), 3 inseminators, and 1 technical official of the Livestock Service. Data analysis was conducted quantitatively and descriptively by calculating the CR and S/C values, which were then compared with the reproductive efficiency standards of beef cattle. Results: The results of the study showed that the CR value in Seruway sub-district, Aceh Tamiang Regency, for the 2023 period was 46.61%, indicating that most cows were successful in getting pregnant with just one AI implementation; the majority (53.39%) were unsuccessful in the first AI, while the Service per Conception (S/C) value of 2.28 indicated that it was less efficient when compared to the ideal standard in cows, which is generally in the range of 1.6–2.0. The average pregnancy was achieved with a very efficient number of inseminations. Conclusion: The implementation of artificial insemination (AI) at the research location showed a fairly good level of reproductive efficiency with CR value of 46.61% and S/C of 2.28.
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