<![CDATA[Background: PO (Peranakan Ongole) cattle are one of the local beef cattle breeds that have become a leading commodity in Tuban District. Lumpy skin disease (LSD) is an infectious disease of cattle that causes significant economic losses to farmers. Purpose: This study aims to detect antibodies in PO cattle in Tuban District. Serum was collected from PO cattle identified from a total of 48 animals from Jatirogo, Merakurak, and Semanding subdistricts. Methods: Detection of anti-LSD antibodies was performed by indirect ELISA using the commercial diagnostic kit 'ID Screen® Capripox Double Antigen Multi-species', manufactured by 'IDvet' (France). Determination of LSD cut-off values for the detection of LSD virus infection was performed using the serum standard positive (S/P) method. Results: The total 48 samples from the three sub-districts, 31 samples (64.58%) were seropositive, and 17 samples (35.42%) were seronegative. Conclusion: The majority of the population had been exposed to LSD virus and had produced LSD virus-specific antibodies, with the highest OD value of 3.8353 from Jatirogo sub-district. Although the cattle had recovered from LSD infection, skin lesions were still found as specific symptoms of LSD. There is a need for a scheduled LSD vaccine administration policy for all PO cattle in Tuban District]]>
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