ALCHEMY Jurnal Penelitian Kimia
Vol 13, No 2 (2017): September

THE EFFECT OF TEMPE FERMENTATION TIME ON THE TOTAL PHENOLIC AND ISOFLAVONE GENISTEIN CONTENTS

Kiki Fransiska Suharto (Jurusan Kimia, Fakultas Sains dan Matematika, Universitas Kristen Satya Wacana, Salatiga, Indonesia)
Hartati Soetjipto (Jurusan Kimia, Fakultas Sains dan Matematika, Universitas Kristen Satya Wacana, Salatiga, Indonesia)
Yohanes Martono (Jurusan Kimia, Fakultas Sains dan Matematika, Universitas Kristen Satya Wacana, Salatiga, Indonesia)



Article Info

Publish Date
01 Sep 2017

Abstract

The objective of this study was to evaluate total phenolic compounds and isoflavone genistein contents during 0 - 9 days of fermentation time. Isoflavone extract were obtained by maceration and fractionation. Total phenolic compounds were measured by Folin ciocalteau method, meanwhile, the isoflavone genistein contents were analyzed by a High Performance Liquid Chromatography. The yield of isoflavone extract and the total phenolic compounds were analyzed using a randomized completely block design and the mean between treatments was compared by the Honestly Significant Difference (HSD) test using significance level of 5 %. The highest amount of total phenolic compounds during the incubation time was obtained on 4 days of fermentation time, i.e., 232.05n ± 7.71 μg/g, while the highest content of isoflavone genistein was obtained on 5 days of fermentation time, i.e., 100.48 μg/g. This research confirms that the fermentation process of tempe induces the total phenolic contents and the production of isoflavone genistein is fluctuating.

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Journal Info

Abbrev

alchemy

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Materials Science & Nanotechnology

Description

ALCHEMY Jurnal Penelitian Kimia is a chemistry journal published by Sebelas Maret University, Surakarta. ALCHEMY Jurnal Penelitian Kimia publishes original research articles or review articles in organic chemistry, inorganic chemistry, analytical chemistry, physical chemistry, biochemistry, and ...