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All Journal EKSAKTA: Journal of Sciences and Data Analysis JURNAL KIMIA SAINS DAN APLIKASI Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Abdimas Indonesian Journal of Cancer Chemoprevention Journal of Degraded and Mining Lands Management The Journal of Pure and Applied Chemistry Research Jurnal Kimia dan Kemasan Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat EduChemia: Jurnal Kimia dan Pendidikan Jurnal Kimia Mulawarman BERITA BIOLOGI Jurnal Bioteknologi & Biosains Indonesia (JBBI) JURNAL KONVERSI Chimica et Natura Acta ALCHEMY Jurnal Penelitian Kimia Akta Kimia Indonesia JKPK (Jurnal Kimia dan Pendidikan Kimia) Berkala Penelitian Hayati Makara Journal of Science Magistrorum et Scholarium: Jurnal Pengabdian Masyarakat Jurnal Manusia dan Lingkungan Molekul: Jurnal Ilmiah Kimia

Hartati Soetjipto

Universitas Kristen Satya Wacana

Author-ID : 384311

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences

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Fatty Acids Composition of Red and Purple Pomegranate (Punica granatum L) Seed Oil Soetjipto, Hartati; Pradipta, Murda; Timotius, KH
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

The aim of this investigation was to determine the content and composition ofÃÂ fatty acid in seed oil of red and purple pomegranate (ÃÂ Punica granatum L). The extraction process was performed by Soxhlet extractor with petroleum ether as solvent. The separation and identification of pomegranate seed oil was done by using GCMS. The total oil content of red and purpleÃÂ pomegranateÃÂ were 128 g/kg d.wÃÂ and 103 g/kg d.w respectively. Both showed the same major fatty acidsÃÂ as palmitic, stearic, oleic, linoleic andÃÂ punisic acid. Oleic acid ( 19-21% ) and linoleic acid ( 20-21% ) were found as the mostÃÂ dominant fatty acids in red pomegranate, whereas purple pomegranate seed oil was dominated by oleic acid ( 41-43% ) and punicic acidÃÂ ( 0-25% ). Neutral lipidÃÂ fraction ofÃÂ red and purple pomegranate seed oils was more dominantthan glycolipid and phospholipid. NeutralÃÂ lipid fractionÃÂ of red and purple pomegranate seed oil wereÃÂ 89 % and 91% respectively.ÃÂ Glycolipid fractionÃÂ of red and purple pomegranate seed oil were 8 % and 5 % , whereas phosholipid fraction of red and purple pomegranate seed oil were 3 % and 4 %. The punicic acid content ofÃÂ total lipid ofÃÂ purple pomegranate seed oil ( PPSO ) (0-25% ) was higher than red pomegranate ( RPSO ) ( 9-16% ). On the contrary neutral lipid of red pomegranate showed higher punicic acid content (54-75%) than the purple pomegranate (14-55% ). Glycolipid of red pomegranate containedÃÂ punicic acidÃÂ ( 0-42%). The punicic acidcontentÃÂ of the phospholipid fraction ofÃÂ red pomegranate was higher ( 0-22 % ) than the one ofÃÂ purple pomegranate (0-2% ).

UPAYA PEMANFAATAN LIMBAH BIJI MANGGA MENJADI PRODUK BERNILAI EKONOMI DI DESA KENDEL KABUPATEN BOYOLALI Soetjipto, Hartati; Linawati, Lilik; Wibowo, Nur Aji
Jurnal Abdimas Vol 19, No 1 (2015)
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (LP2M), Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Limbah biji mangga yang terbuang percuma dapat diolah menjadiberbagai produk yang memiliki nilai jual seperti minyak, sabun dan tepung bijiÂ mangga. Hasil penelitian di laboratorium Kimia FSM UKSW telah diaplikasikan di kelompok ibu-ibu PKK desa Kendel kabupaten Boyolali sejak Aprilâ€“Oktober 2014. Minyak biji mangga yang diperoleh dengan metoda ekstraksi, dibuat sabunÂ mandi dengan metoda dingin sedangkan tepung biji mangga diperoleh denganÂ metoda pengeringan. Tujuan dari pelatihan ini adalah untuk meningkatkanÂ kemampuan SDM ibu-ibu yang tergabung dalam kelompok PKK desa Kendel agarÂ mampu menghasilkan produk barang layak jual untuk membantu perekonomianÂ keluarga.Kata kunci: Kendel, minyak biji mangga, sabun alami, tepung biji mangga

Toksisitas Ekstrak Kasar Bunga dan Daun Ketepeng Cina (Senna alata L. Roxb.) terhadap Larva Udang Artemia salina Leach Soetjipto, Hartati; Kristijanto, A. Ign.; Asmorowati, Rica Susy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Extract of Candlebush (Senna alata L. Roxb) flowers and leaves show toxicity effect on Artemia salina Leach. The crude extract was obtained by maceration method using methanol 80% and followed by partition into hexane, chloroform and ethylacetate. Data was analized using Randomized Completely Block Design (RCBD) Sub Sampling, that consisted of six treatments, five replications and three sub samples. To compare the differences between the mortality rater of A. salina, the Honestly Significant Differences (HSD) at 5% level of significance was used. Furthermore, to determine the LC50, the probit analysis was used. The results of this study show that the most effective toxin from all fractions of S. alata was the hexane fraction of the leaves extract. The LC50 of S. alata crude extract of leaves was 187,72 ppm (hexane fraction) and 290,34 ppm (EtOAc fraction). The crude extract of S. alata flowers toward A. salina were 210,77 ppm (hexane fraction) and 354,49 ppm (EtOAc fraction). In general, this study showed that all fractions which were tested had the toxic activity on A. salina.

ISOLASI DAN ANALISIS GENISTEIN DARI TEMPE BUSUK MENGGUNAKAN METODE KROMATOGRAFI KOLOM Hartati Soetjipto; Yohanes Martono; Zulfa Yuniarti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 5 No. 1 (2018): June 2018
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Isolation and Analysis of Genistein of Overripe Tempe using Column Chromatography MethodABSTRACTGenistein is one of the aglycone isoflavone compounds in tempe that has various biochemical activities, including anticancer, antitumor, and antioxidants. Commonly used isoflavone extraction methods resulted in isoflavone crude extract. The aim of this study was to isolate the genistein of overripe tempe through determining the appropriate combination of mobile phases in genistein isolation and the determination of genistein content in both crude extract and isolate. The overripe tempe was first extracted, then genistein was isolated from the crude extract using column chromatography method. The determination of mobile phase combination was done by Thin Layer Chromatography while the genistein content was quantitatively determined by using High Performance Liquid Chromatography. The results showed that the appropriate combination of mobile phase for genistein isolation was chloroform : methanol (15 : 1, v/v). Genistein content in the crude extract and isolates were 4737.50 and 31.36 Î¼g/g extract, respectively. The genistein purity in the isolates was 63.80%, while the purity in the isoflavone extract was 31.98%.Keywords: genistein, HPLC, isoflavone, overripe tempe, TLCÂ ABSTRAKGenistein merupakan salah satu senyawa isoflavon aglikon dalam tempe yang memiliki bermacam-macam aktivitas biokimia, diantaranya antikanker, antitumor, dan antioksidan. Metode ekstraksi isoflavon yang umum diterapkan, menghasilkan ekstrak kasar isoflavon yang masih berupa campuran. Tujuan dari penelitian ini adalah untuk mengisolasi genistein dari tempe busuk melalui tahap penentuan kombinasi fase gerak yang tepat dalam isolasi genistein serta penentuan kandungan genistein baik dalam ekstrak kasar maupun isolat. Tempe busuk mula-mula diekstrak, selanjutnya genistein diisolasi dari ekstrak kasar menggunakan metode kromatografi kolom. Penentuan kombinasi fase gerak dilakukan secara Kromatografi Lapis Tipis, sedangkan kandungan genistein secara kuantitatif ditentukan dengan menggunakan Kromatografi Cair Kinerja Tinggi. Hasil penelitian menunjukkan bahwa kombinasi fase gerak yang tepat untuk isolasi genistein adalah kloroform : metanol (15 : 1, v/v). Kandungan genistein dalam ekstrak kasar dan isolat genistein berturut-turut sebesar 4737,50 dan 31,36 Î¼g/g ekstrak. Kemurnian genistein dalam isolat adalah sebesar 63,80%, sedangkan kemurniannya dalam ekstrak isoflavon adalah sebesar 31,98%.Â Kata Kunci: genistein, HPLC, isoflavon, tempe busuk, KLT

Pengaruh Proses Degumming dan Netralisasi Terhadap Sifat Fisiko Kimia dan Profil Asam Lemak Penyusun Minyak Biji Gambas (Luffa acutangula Linn.) Antonius Rizky Meilano; Hartati Soetjipto; Margareta Novian Cahyanti
Chimica et Natura Acta Vol 5, No 2 (2017)
Publisher : Departemen Kimia

Penelitian mengenai minyak biji gambas umumnya menggunakan minyak kasar, pemurnian terhadap minyak biji gambas masih belum dilakukan. Tujuan dari penelitian ini adalah untuk menentukan efek proses degumming dan netralisasi terhadap sifat fisiko kimia dan kandungan minyak biji gambas (Luffa acutangula Linn.). Metode yang digunakan dalam memperoleh minyak biji gambas yaitu maserasi dengan pelarut heksana. Sifat fisiko-kimia minyak biji gambas sebelum dan sesudah pemurnian ditentukan berdasarkan SNI 01-3555-1998. Sesudah pemurnian minyak biji gambas mengalami perubahan warna, massa jenis, kadar air, bilangan asam, bilangan peroksida, dan bilangan penyabunan. Analisa profil asam lemak dilakukan dengan instrumen GC-MS. Adanya pemurnian tidak berpengaruh terhadap komponen utama asam lemak penyusun minyak biji gambas.

The quantitative soil quality assessment for tobacco plant in Sindoro mountainous zone S Supriyadi; R Sudaryanto; J Winarno; S Hartati; I S Jamil
Journal of Degraded and Mining Lands Management Vol 1, No 3 (2014)
Publisher : Brawijaya University

The long-term cultivation of tobacco (Nicotiana tabacum) plant in the Sindoro mountainous zone of Central Java has resulted in soil quality degradation that could affect economic development in the region if sustainable production practices are not identified. The objective of the study was to identify appropriate indicators for assessing soil quality on tobacco plant. The quantitative soil quality indicators were total organic-C, pH, available P and available K (chemical), soil depth, bulk density, AWC (available water capacity) and soil aggregate stability (physical), and qCO2 (soil respiration), MBC (microbial biomass carbon) (biological). The decreases in the soil aggregate stability, available water capacity, cation exchange capacity, soil respiration, microbial biomass carbon and total organic-C; or increases in bulk density (compaction), available P, available K and total nitrogen indicated the decrease in soil quality due to long-term tobacco production. The result of this research showed that the change of soil quality had occurred in Sindoro Mountain. The Soil Quality Index (SQI) for three land use systems in Sindoro mountain (forest, mixed farm, and tobacco) were 0.60, 0.47, and 0.57, respectively. The comparison of these rates with soil quality classes showed that the soil quality presented moderate to good level of quality; class SQI.

Effects of Refining on the Characteristics of Suri Cucumber (Cucumis melo L. var. reticulatus Naudin) Seed Oil Hartati Soetjipto; Laili Nurrohmah; Agustinus Ignatius Kristijanto
The Journal of Pure and Applied Chemistry Research Vol 9, No 3 (2020): Edition September-December 2020
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2020.009.03.533

The oil refining of Suri cucumber seed oil has been developed and tested. Crude oil was obtained by using soxhlet extraction in n-hexane, then was refined using chemical method by degumming and neutralization. This study aims are to determine the oil yield before and after refining, the oil’ physicochemical characteristic before and after refining and to determine the oil profile before and after refining by the Gas Chromatography-Mass Spectrometry (GCMS) method. The refining process cause the color of the oil become paler, but do not significantly affect on the water content and density. However, the process effect to decreasing of the acid value (5.547±0 mg NaOH/g oil to 0.5±0.14 mg NaOH/g oil) and the peroxide number (0.5077±0.036meq O2/g oil became 0.145 ± 0.036meq O2/g oil). Conversely, the saponification value increase (224.06±0.69mg KOH/g oil to 240.17±1.74mg KOH/g oil). GCMS analysis shows that refining process have effects on the composition of fatty acid in the oil.

EKSTRAKSI MINYAK BIJI JENGGER AYAM (Celosia argentea var. cristata) DAN KARAKTERISASI DAN KANDUNGANNYA Hartati Soetjipto; Novenda Pramesti Ayuningtyas; November Rianto Aminu
Jurnal Kimia dan Kemasan Vol. 42 No. 2 Oktober 2020
Publisher : Balai Besar Kimia dan Kemasan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24817/jkk.v42i2.6138

Tujuan dari penelitian ini adalah memperoleh rendemen minyak biji jengger ayam (Celosia argentea var. cristata) dengan metode ekstraksi berkelanjutan menggunakan pelarut heksana serta menentukan komposisi kimia minyak biji jengger ayam menggunakan metode Gas Chromatography-Mass Spectrometry (GC-MS) sebelum dan setelah pemurnian. Hasil rendemen minyak biji jengger ayam sebelum dan setelah pemurnian adalah 6,79 ± 0,27% dan 1,88 ± 0,01%. Tujuh asam lemak teridentifikasi dalam minyak kasar, sedangkan empat asam lemak teridentifikasi setelah minyak dimurnikan. Asam palmitat 3,38%; skualen 89,67%; dan asam linoleat 6,58% merupakan asam lemak dominan dalam minyak biji jengger ayam hasil pemurnian.

Profil Asam Lemak Dan Karakterisasi Minyak Biji Labu Kuning (Cucurbita moschata D.) Hartati Soetjipto; Trisna Anggreini; Margareta Novian Cahyanti
Jurnal Kimia dan Kemasan Vol. 40 No. 2 Oktober 2018
Publisher : Balai Besar Kimia dan Kemasan

Tujuan penelitian ini adalah memperoleh rendemen optimal minyak biji labu kuning ditinjau dari metode ekstraksi dan pelarut, menentukan sifat fisika kimia minyak biji labu kuning, serta menentukan komposisi minyak biji labu kuning dengan metode Gas Chromatography-Mass Spectrometry (GC-MS). Data dianalisis dengan menggunakan Rancangan Acak Kelompok (RAK), 4 perlakuan dan 6 kali ulangan. Sebagai perlakuan adalah penggunaan metode dan jenis pelarut sedangkan sebagai kelompok adalah waktu analisis. Pengujian antar rataan perlakuan dilakukan dengan uji Beda Nyata Jujur (BNJ) dengan tingkat kebermaknaan 5%. Hasil penelitian menunjukkan bahwa rendemen minyak biji labu kuning optimal diperoleh dari penggunaan metode soxhlet dengan pelarut heksana yaitu sebesar 36,65 ± 2,20%. Minyak yang diperoleh berwarna merah kecoklatan berbau khas biji labu kuning dengan kadar air minyak 3,86 ± 1,21%; massa jenis minyak 0,83 ± 0,03 g/ml; kadar asam lemak bebas 6,56 ± 2,59%; bilangan asam 13,06 ± 5,15 mg KOH/g; bilangan peroksida 6,54 ± 0,09 meq O2/kg; dan bilangan penyabunan 199,44 ± 0,47 mg KOH/g. Hasil analisis GC-MS menunjukkan bahwa minyak biji labu kuning tersusun atas 4 komponen utama yaitu : asam palmitat (24,64%); asam linoleat (57,96%); asam stearat (6,83%); dan skualena (2,13% dan 8,44%).

Solvent Optimization For Genistein Isolation Of â€œRotten Tempeâ€ By High Performance Liquid Chromatography Method Cucun Alep Riyanto; Hartati Soetjipto
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 17, ISSUE 2, August 2017
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/eksakta.vol17.iss2.art3

Genistein is a soy isoflavone that has been known have anticancer properties. The aim of this research is to determine the right combination of solvents to extract isoflavones in rotten tempe and determine the genistein content of the extraction process by the High Performance Liquid Chromatography (HPLC) method. Isoflavone extraction process was done using a mixture of methanol and chloroform solutions. Isoflavone extracts obtained will be analysed using High Performance Liquid Chromatography (HPLC) method to determine the content of genistein. The optimum isoflavone extract was obtained from chloroform:methanol (10:1 v/v) solvent ratio. Where as the highest contents of genistein from the 4th day â€œrotten tempeâ€ as 26.199 ± 25.146 (mg/g).

Co-Authors A Ign Kristijanto A. Ign Kristijanto Antonius Rizky Meilano Apriyanti Tindage Asmorowati, Rica Susy Augustinus Ignatius Kristijanto Cucun Alep Riyanto Cucun Alep Ryanto Devina Intan Sari Dewi Kurniasih Arum Kusuma Hastuti Divina Anindya Della Diyonisio Kevin Elisando Febriyanti, Teresa Franciska Alvina Hanny Hafiar Hoo Sheren Hartono I S Jamil Imelda Wadu Indah Tri Susilowati J Winarno KH Timotius Kiki Fransiska Suharto Krisdayanti, Mitra Kristijanto, Agus Ign. Laili Nurrohmah Laili Nurrohmah Lany Indrayani Lusiawati Dewi Lydia Sihasale Margareta Novian Cahyanti Mitra Krisdayanti Murda Pradipta November Rianto Aminu Novena Risnalani Rintank Constani Novenda Pramesti Ayuningtyas Purwoko, Agus R Sudaryanto Riska Cahyaningtyas S Supriyadi Salsabila, Unik Hanifah Sentot Adi Prayitno Sri Hartini Sri Hartini Suprihati Suprihati Suprihati Susanti Pudji Hastuti Teguh Wahyono Teresa Febriyanti Tiffany Febetania Widodo Trisna Anggreini Widhi Nugroho Meindra Yayi Suryo Prabandari Yoga Andika Putra Yoga Andika Putra Yohanes Martono Yuliawati Zulfa Yuniarti

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