Antibacterial Activity of Germacrone Sesquiterpene from Curcuma Xanthorrhiza Rhizomes

Hartiwi Diastuti (Jurusan Kimia, FMIPA, Universtas Jenderal Soedirman, Jl. Dr. Soeparno 61, Karangwangkal, Purwokerto 53125 telp. [phone])
Yana Maolana Syah (Jurusan Kimia, FMIPA, Universtas Jenderal Soedirman, Jl. Dr. Soeparno 61, Karangwangkal, Purwokerto 53125 telp. [phone])
Lia Dewi Juliawaty (Jurusan Kimia, FMIPA, Universtas Jenderal Soedirman, Jl. Dr. Soeparno 61, Karangwangkal, Purwokerto 53125 telp. [phone])
Marlia Singgih (Jurusan Kimia, FMIPA, Universtas Jenderal Soedirman, Jl. Dr. Soeparno 61, Karangwangkal, Purwokerto 53125 telp. [phone])

ALCHEMY Jurnal Penelitian Kimia
Vol 12, No 2 (2016): September

The aim of this research was to isolate and indentify the terpenoid compound from Curcuma xanthorrhiza rhizomes and its antibacterial activity. Isolation was carried out by using vacuum liquid chromatography and centrifugal chromatography. The structure was determined by NMR spectroscopy (1H-NMR, 13C-NMR 1D and 2D), then compare with data from literatures. Antibacterial test was carried out by using  microdillution methods and evaluated against eight bacteria. They are Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Enterobacter aerogenes, Pseudomonas aeruginosa, Salmonella typhi, Shigella dysentriae and Vibrio cholerae. The result showed that the isolate was a white crystal which was indetified as germacron-type sesquiterpene. Germacron have highest activity againts P. aeruginosa, MIC 15.6 µg/mL and MBC 31.2 µg/mL.