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FORMULATION AND NANOEMULSION OF NUTMEG ESSENTIAL OIL (Myristica fragrans Houtt.) AND SUNSCREEN ACTIVITY TEST USING UV-VIS SPECTROPHOTOMETRY Sheiliyani, Cindy; Hidayat, Arif Nur; Rastuti, Undri; Chasani, Moch; Diastuti, Hartiwi
SPIN JURNAL KIMIA & PENDIDIKAN KIMIA Vol. 5 No. 2 (2023): Juli - Desember 2023
Publisher : UIN Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/spin.v5i2.7871

Nutmeg essential oil (Myristica fragrans Houtt) is a natural ingredient that has potential as a sunscreen. This research aims to formulate and characterize nutmeg essential oil nanoemulsion and test the sunscreen activity of nutmeg essential oil and its nanoemulsion. The essential oil and nanoemulsion formulas were made with 5 variations of essential oil concentrations namely F0 (0%), F1 (1%), F2 (2%), F3 (4%) and F4 (6%). The nanoemulsion characterization includes organoleptic test, pH measurement, nanoemulsion type examination, viscosity measurement, percent transmittance test, particle size distribution measurement, centrifugation test, freez-thaw cycle test. Testing the potential of sunscreen was carried out using a UV-Vis spectrophotometer at a wavelength range of 290-400 nm with ethanol as a blank. The results of the nanoemulsion organoleptic characterization showed a clear and stable appearance. Measurements of pH, type of nanoemulsion, viscosity, percent transmittance, and particle size of nanoemulsion preparations showed good results and were in accordance with the parameters. The nutmeg essential oil nanoemulsion had better SPF, %Te and %Tp values than the essential oil. Nanoemulsion preparations that had the best sunscreen activity in this study were F4 (6%) with an SPF value of 1.538; %Te value of 37.375%; and %Tp value of 80.732%.

Two Sesquiterpenes from n-Hexane Fraction of Curcuma soloensis Rhizomes and Their Antimicrobial Activities Diastuti, Hartiwi; Asnani, Ari; Lestari, Puji; Astuti, Teni; Nurmalia, Naela; Hidayat, Ade Sholeh
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 1, May 2024
Publisher : Syarif Hidayatullah State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i1.36613

Curcuma soloensis is one of the medicinal plants that has the potential to be a source of bioactive compounds. The antimicrobial study of the bioactive compounds from C. soloensis was still limited. This study aimed to isolate the bioactive compounds from C. soloensis rhizomes and to evaluate their potential as antimicrobial agents. C. soloensis rhizome extraction was done using the maceration method with acetone and then fractionated with n-hexane: methanol (1:1). The compounds were separated and purified using vacuum liquid chromatography and radial chromatography. The structure of the isolated compounds was determined using the nuclear magnetic resonance (1H and 13C NMR) spectroscopy analysis and comparison with literature data. Antimicrobial activity assays of the extract, n-hexane fraction, and isolated compounds were carried out by microdilution methods against Escherichia coli, Staphylococcus aureus, Candida albicans, and Malaszezia furfur. Two bioactive compounds from the n-hexane extract of C. soloensis rhizome have been isolated: ar-curcumene and ar-turmerone. Antimicrobial test results on acetone extract, n-hexane fraction, and isolated compounds showed that ar-turmerone had the highest activity against S. aureus with a MIC value of 15.6 μg / mL. In comparison, ar-curcumene showed the same activity against all test microbes with a MIC value of 62.5 μg / mL. This study showed that secondary metabolite compounds of C. soloensis rhizomes have the potential to be developed as antimicrobial agents.

Toxicity Test of Nanoemulsions of Nutmeg Fruits and Leaves Essential Oil against Artemia salina Leach and Its Cytotoxicity Test against Breast Cancer Cells T47D Rastuti, Undri; Diastuti, Hartiwi; Widyaningsih, Senny; Chasani, Moch; Sheiliyani, Cindi; Rahmasari, Anisa; Fajriyah, Bunga Sita Roihanul; Mesayu, Puspa Rahma; Habibie, Ranti Kamila
Indonesian Journal of Chemistry Vol 24, No 5 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijc.91077

Nutmeg (Myristica fragrans Houtt) is a widely known spice plant, which has been reported to offer several benefits. Therefore, this study aims to develop and analyze nanoemulsions of nutmeg leaves and fruit essential oil, as well as determine their toxicity and cytotoxicity. Nanoemulsions were formulated with varying concentrations of essential oil, including 0, 1, 2, 4, and 6%. Characterization included organoleptic assessment, pH measurement, type examination, viscosity testing, transmittance analysis, particle size distribution measurement, centrifugation, and freeze-thaw cycle test. Toxicity testing results using the brine shrimp lethality test (BSLT) showed that nanoemulsions were toxic except NF F4 with high toxicity. Cytotoxicity testing on T47D breast cancer cells showed moderate activity for NF F4 nanoemulsions (IC50: 34.363 ppm), while NL nanoemulsions were deemed inactive (IC50: 33576.430 ppm). In addition, the organoleptic characteristics of all nanoemulsions were stable, and most parameters met the desired standards. Based on the results, further studies exploring nanoemulsions with natural products must be carried out to determine their advantages, specifically in the development of sciences.

Isolasi Senyawa Metabolit Sekunder dari Fraksi Etil Asetat Rimpang Bangle (Zingiber cassumunar Roxb.) serta Uji Aktivitas terhadap Candida albicans Diastuti, Hartiwi; Mufida, Zeni Lutfi; Purwati
Jurnal Sains dan Edukasi Sains Vol. 7 No. 1 (2024): Jurnal Sains dan Edukasi Sains
Publisher : Faculty of Science and Mathematics, Universitas Kristen Satya Wacana, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24246/juses.v7i1p29-36

Bangle (Zingiber cassumunar Roxb) merupakan salah satu tanaman obat Indonesia yang banyak dimanfaatkan masyarakat untuk bahan pembuatan jamu dan pengobatan tradisional seperti mengobati demam, luka, dan nyeri perut. Senyawa metabolit sekunder dari rimpang bangle diketahui memiliki berbagai macam aktivitas biologi, salah satunya adalah sebagai antijamur. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi senyawa bioaktif dari fraksi etil asetat rimpang bangle serta menguji aktivitasnya terhadap jamur Candida albicans dengan metode difusi sumuran. Tahapan isolasi senyawa meliputi maserasi sampel menggunakan pelarut aseton yang selanjutnya ekstrak aseton diekstraksi partisi cair-cair dengan campuran n-heksana:metanol (1:1), kemudian fraksi metanol dipartisi kembali dengan etil asetat:air (1:1), fraksi etil asetat selanjutnya difraksinasi dengan kromatografi kolom cair vakum dengan elusi bergradien menggunakan campuran pelarut n-heksana dan etil asetat yang ditingkatkan kepolarannya, dan pemisahan senyawa menggunakan kromatografi sentrifugal dengan eluen n-heksana:etil asetat (7:3). Senyawa hasil isolasi diuji fitokimia, kemudian diidentifikasi komponennya menggunakan Liquid Chromatography-Mass Spectrometer (LC-MS). Uji fitokimia menunjukkan bahwa isolat memberikan hasil positif pada uji flavonoid. Hasil analisis LC-MS memperlihatkan bahwa isolat mengandung komponen utama yang teridentifikasi sebagai flavenochromane C pada waktu retensi 6,65 menit, dengan luas area 77,51%, rumus molekul C21H20O6 dan berat molekul 369,13. Hasil pengujian antijamur diketahui isolat menunjukkan aktivitas yang lemah terhadap C. albicans dengan zona hambat 5,25 mm.

PEMANFAATAN PEWARNA ALAMI DALAM PRODUKSI KERTAS DAUR ULANG DI BANK SAMPAH SRAYAN MAKARYA Asnani, Ari; Diastuti, Hartiwi; Delsy, Eva Vaulina Yulistia; Handoko, Waluyo
Jurnal Abdimas Ilmiah Citra Bakti Vol. 6 No. 2 (2025)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v6i2.4707

Bank Sampah (BS) Srayan Makarya beroperasi dengan menerima sampah atau mengambil sampah dari anggotanya. Limbah kertas tergolong tinggi karena kertas merupakan bahan yang sangat umum digunakan dalam berbagai aktivitas dan kehidupan sehari-hari. Untuk mengatasi penumpukan limbah kertas, maka dilakukan kegiatan pengabdian kepada masyarakat dengan tujuan pengolahan limbah kertas menjadi kertas daur ulang, difusi teknologi ekstraksi pewarna alami, serta aplikasi pewarna alami untuk pewarnaan kertas daur ulang. Mitra kegiatan abdimas adalah para anggota BS Srayan Makarya yang berdomisili di Bobosan, Purwokerto Utara, Jawa Tengah. Pelaksanaan kegiatan meliputi sosialisasi, pelatihan pembuatan kertas daur ulang, demonstrasi ekstraksi pewarna alami, pelatihan aplikasi pewarna alami untuk pewarnaan kertas daur ulang, praktek mandiri, dan evaluasi kegiatan melalui pre-test dan post-test. Pembuatan kertas daur ulang menggunakan limbah kertas HVS, kertas buram, kertas folio bergaris, dan kardus bekas. Pewarna alami yang digunakan adalah ekstrak aqueous dari daun ketapang, daun rengat, kayu tingi, dan kayu secang. Hasil kegiatan adalah produk kertas daur ulang dengan pewarna alami. Hasil evaluasi kegiatan menunjukkan peningkatan pengetahuan peserta terkait alat (100%), bahan (100%), dan prosedur pembuatan kertas daur ulang (100%). Hasil evaluasi peminatan menunjukkan bahwa sebagian besar peserta (41,7%) melakukan kegiatan untuk meningkatkan pengetahuan dan ketrampilan, sedangkan yang berminat untuk berwirausaha kertas daur ulang sebanyak 33,3%. Aplikasi pewarna alami pada pembuatan kertas daur ulang di BS Srayan Makarya dapat memicu kreativitas mitra untuk mengolah limbah kertas menjadi produk fungsional yang bernilai ekonomi.

Two Sesquiterpenes from n-Hexane Fraction of Curcuma soloensis Rhizomes and Their Antimicrobial Activities Diastuti, Hartiwi; Asnani, Ari; Lestari, Puji; Astuti, Teni; Nurmalia, Naela; Hidayat, Ade Sholeh
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 10, No. 1, May 2024
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v10i1.36613

Curcuma soloensis is one of the medicinal plants that has the potential to be a source of bioactive compounds. The antimicrobial study of the bioactive compounds from C. soloensis was still limited. This study aimed to isolate the bioactive compounds from C. soloensis rhizomes and to evaluate their potential as antimicrobial agents. C. soloensis rhizome extraction was done using the maceration method with acetone and then fractionated with n-hexane: methanol (1:1). The compounds were separated and purified using vacuum liquid chromatography and radial chromatography. The structure of the isolated compounds was determined using the nuclear magnetic resonance (1H and 13C NMR) spectroscopy analysis and comparison with literature data. Antimicrobial activity assays of the extract, n-hexane fraction, and isolated compounds were carried out by microdilution methods against Escherichia coli, Staphylococcus aureus, Candida albicans, and Malaszezia furfur. Two bioactive compounds from the n-hexane extract of C. soloensis rhizome have been isolated: ar-curcumene and ar-turmerone. Antimicrobial test results on acetone extract, n-hexane fraction, and isolated compounds showed that ar-turmerone had the highest activity against S. aureus with a MIC value of 15.6 μg / mL. In comparison, ar-curcumene showed the same activity against all test microbes with a MIC value of 62.5 μg / mL. This study showed that secondary metabolite compounds of C. soloensis rhizomes have the potential to be developed as antimicrobial agents.

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