Faktor Exacta
Vol 6, No 3 (2013)

ISOLASI DAN IDENTIFIKASI BAKTERI PENGHASIL IAA (Indole-3-Acetid Acid) DARI TANAH DAN AIR DI SITUGUNUNG, SUKABUMI

ZAKIAH FITHAH A’INI (Unknown)



Article Info

Publish Date
05 Oct 2015

Abstract

The purpose of this research study to isolate and identify bacterial soil and water producing IAA (Indole-3-Acetid Acid) Situgunung, Sukabumi potential biofertilizer. This research study method consists of six stages: soil and water sampling, manufacturing pure isolates, gram stain for bacterial identification, testing bacteria producing IAA (Indole-3-3Acetid Acid) with the Salkowski reagent, IAA standard curve creation, and storage of bacterial isolates obtained in IPB Culture Collection. Soil samples taken from the root zone 5 (five) trees Rasamala (Altingia excelsa) around the hiking trail to the waterfall Cimaracun, Sawer, Kembar, Situgunung, and Situgunung lake, of HM 0 HM 2, HM 5, HM 6, HM 9. Isolation of auxin-producing bacteria using media Nutrient Broth (NB) and Nutrient Agar (NA), which both added 0.0204 grams of L-tryptophan (Trp) per 100 mL of distilled water. The results of the purification of pure isolates obtained 26 NA + Trp medium, 15 isolates from soil samples, while 11 isolates from water samples. Has been fully tested with Salkowski reagent and measurement accuracy of a standard curve with a value of 0.972. The test results showed that 9 out of 10 isolates produced IAA exogenous to the value of the highest concentrations of 9.92 ppm produced by isolates A4.01. Keywords. Bacteria IAA, bacterial soil and water, Salkowski reagent.

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Journal Info

Abbrev

Faktor_Exacta

Publisher

Subject

Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Electrical & Electronics Engineering Industrial & Manufacturing Engineering

Description

Faktor Exacta is a peer review journal in the field of informatics. This journal was published in March (March, June, September, December) by Institute for Research and Community Service, University of Indraprasta PGRI, Indonesia. All newspapers will be read blind. Accepted papers will be available ...