Biota
Vol 3 No 1 (2017): Vol 3 No 1 Januari 2017

SKRINING AKTIVITAS ANTIBAKTERI DAUN KELOR (Moringa oleifera), DAUN BIDARA LAUT (Strychnos ligustrina Blume), DAN AMOXICILIN TERHADAP BAKTERI PATOGEN Staphylococcus aureus

Edi Suriaman (Prodi D III Analis Kesehatan, Akademi Analis Kesehatan Malang, Jl. Terusan Danau Sentani No. 97 Malang, Jawa Timur)



Article Info

Publish Date
30 Jan 2017

Abstract

Exploration of a variety of plants as a source of antibacterial has been done, but it is not all plants effective to inhibit the growth of bacteria. This study aims to determine the difference between the ability of the antibacterial activity of plant leaves Moringa oleifera and Strychnos ligustrina Blume against Staphylococcus aureus bacteria growth. This research is an experiment with a completely randomized factorial design (CRD) 3x4 with four replicates. The materials use is ethanol extract of the leaves of Moringa leaf extract, S. ligustrina, and amoxicillin. Testing the antibacterial activity performed on Mueller-Hinton agar media with the diffusion method. The analysis and interpretation of results were done by ANOVA 95% (α: 0.05). Susceptibility bacteria to antibiotics, and plant extracts assessed according to standard NCCLS (National Committee for Clinical Laboratory Standards). The antibacterial agent (ethanol extract of Moringa leaf, ethanol extract of S. ligustrina leaf, and amoxicillin) and concentrations influence significantly inhibit the growth of S. aureus bacteria. S. aureus bacteria are sensitive only at 100% concentration with inhibition zone diameter 21.5 ± 5:35 mm, when given the leaf extract of S. ligustrina, and at a concentration of 75% with a diameter of 14.3 ± 1.32 mm is intermediate. The bacteria behave-resistant to the compound of M. oleifera leaf extract and amoxicillin at all concentrations. Diameter of zones of inhibition amoxicillin equal 9.99 ± 2.02 mm with 100% concentration treatment.  While the treatment of M. oleifera leaf extract, at a concentration of 75% and 100%, resulting in the largest inhibition zone each is 3.9 ± 0.64 and 3.82±0.57 mm.

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Journal Info

Abbrev

biota

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Materials Science & Nanotechnology Medicine & Pharmacology

Description

Jurnal biota is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biology, with particular attention paid to the exploration and biodiversity Wetland tropical—and especially Indonesian—biodiversity. Original research ...