Jurnal Penelitian Pertanian Tanaman Pangan
Vol 34, No 2 (2015): Agustus 2015

Distinguishing Rice Genotypes using Morphological, Agronomical,

Susanto, Untung (Indonesian Center for Rice Research (ICRR) Street 9th, Subang, West Java, Indonesia)
Rohmah, Nofi Anisatun (Indonesian Center for Rice Research (ICRR) Street 9th, Subang, West Java, Indonesia)
Mejaya, Made Jana (Indonesian Center for Rice Research (ICRR) Street 9th, Subang, West Java, Indonesia)



Article Info

Publish Date
12 Nov 2015

Abstract

Complete data on characteristics of a rice variety is very important to  race the authenticity of the variety at the field. Sometimes a name of a variety had changed, due to the informal  seed distribution among farmers. This could become problem in the property right of the variety. Distinguishing among rice varieties using only morphological and agronomical traits are sometimes not sufficient. Currently, molecular markers such as SSR (Simple Sequence Repeats) and SNP (Single Nucleotide Polymorphism) markers have become available and are powerfull to distinguish rice genotypes. This research was aimed to distinguish nine rice varieties grown by farmers, using morphological characters (47 traits), agronomical characters (9 traits), SSR markers (12 primer pairs, related with important traits of rice plant), and 384 SNP markers, and to compare the effectiveness of each technique in distinguishing among genotypes. A field experiment was conducted in Ranca Jaya village, Patok Beusi, Subang, West Java during Wet Season (WS) of 2011/2012, using a Randomized Complete Block Design in three replications. A modified CTAB method was used to extract DNA for detection using 12 SSR markers and 384 SNP markers. The results revealed that the use of SSR markers that were linked to certain genes was more accurate than that of the SNP markers, agronomic, and morphological characters, in distinguishing differences among the 9 rice genotypes. The complete data of morphologic, agronomic, and molecular are useful to distinguish the authenticity of a variety in order to protect the intelectual property right attached on the variety.

Copyrights © 2015






Journal Info

Abbrev

jpptp

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Jurnal Penelitian Pertanian Tanaman Pangan merupakan publikasi yang memuat makalah ilmiah primer hasil penelitian tanaman pangan (padi dan palawija). Redaksi menerima makalah dari peneliti Puslitbang Tanaman Pangan, Balai Pengkajian Teknologi Pertanian (BPTP), maupun perguruan tinggi. Makalah yang ...