Cloning of a Transglutaminase Gene from Streptomyces thioluteus TTA 02 SDS 14

Seprianto Seprianto (Bogor Agricultural University)
Dewi Seswita Zilda
Yusro Nuri Fawzya
Suharsono Suharsono
Puspita Lisdiyanti
Agustinus Robert Uria

Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Vol 11, No 1 (2016): May 2016

Microbial Transglutaminase (MTGase, EC 2.3.2.13) is an enzyme that catalyzes the transfer of acyl group. Many microbial strains that produce MTGase belong to Streptomyces members. This research was aimed at cloning of a MTGase gene. PCR–based screening of ten MTGase-producing streptomyces isolates from soil in West Nusa Tenggara led to detection of one potential isolate, designated as TTA 02 SDS 14. The partial  MTGase-encoding gene (470 bp)  was amplified by PCR and sequenced. The sequence result indicate its similarity of 93 % with that of Streptomyces cinnamoneus. The 16S rRNA gene analysis showed its identity as Streptomyces thioleteus. Fosmid-based construction of a genomic library from the isolate  and subsequent screening led to the isolation of  a ~40-Kb fosmid harboring a MTGase gene.