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ISOLASI DAN PENAPISAN BAKTERI SELULOLITIK DARI BERBAGAI JENIS TANAH SEBAGAI PENGHASIL ENZIM SELULASE Seprianto, Seprianto
Indonesian Journal of Biotechnology and Biodiversity Vol 1, No 2 (2017): Indonesian Journal of Biotechnology and Biodiversity
Publisher : Indonesian Journal of Biotechnology and Biodiversity

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AbstractCellulose is an enzyme that hydrolyzes Î²-1,4-glycosidic bonds on cellulose molecules to produce glucose. The utilization of cellulase enzymes is currently increasing in the food, paper , detergent and agricultural industries. This research was aimed at obtaining cellulolytic bacterial isolates capable of producing cellulose enzyme where enzyme activity was measured daily by DNS method and to add bacterial isolate collection which will be used for further research. Screening of cellulolytic bacteria from soil led to detection or one potential isolate, designated as 6.2 showed highest activity among others which occurred on the 11th day with activity value of 0,0189 Î¼mol/minute and the lowest activity of cellulase enzyme was not observed its activity, while the highest dissolved protein content occurred on the sixth day with activity of 0,408240784 mg/ml and the lowest protein content on day 2th of 0,167673552 mg / ml. The highest activity of specific enzyme occurred on the 11th day with a value of 0,0538 U/mg, while the lowest activity of specific enzyme was not activity with value of 0 U/mg.Â Keywords : screening, cellulolytic bacteria, celluloseÂ AbstrakEnzim selulase merupakan enzim yang dapat menghidrolisis ikatan Î²-1,4-glikosidik pada molekul selulosa sehingga menghasilkan glukosa. Pemanfaatan enzim selulase saat ini semakin meningkat dalam dunia industri makanan, industri kertas, industri detergen dan industri pertanian. Penelitian ini bertujuan untuk mendapatkan spesies bakteri selulolitik yang potensial menghasilkan enzim selulase dan melihat aktivitas enzim dengan metode pengukuran DNS serta menambah koleksi isolat bakteri dari biakan murni yang akan digunakan untuk penelitian selanjutnya. Dari hasil penelitian penapisan bakteri selulolitik yang diisolasi dari berbagai jenis tanah didapatkan satu isolat yang potensial yaitu Isolat 6.2 menunjukan aktivitas lebih baik diantara yang lainnya dengan aktivitas enzim ektrak kasar tertinggi terjadi pada hari ke-11dengan nilai 0,0189 Âµmol/menit dan aktivitas enzim selulase terendah tidak teramati aktivitas enzimnya atau sama dengan nilai 0 Âµmol/menit, sedangkan kadar protein terlarut tertinggi terjadi pada hari ke-6 dengan nilai 0,408240784 mg/ml dan kadar protein terendah pada hari ke-2 sebesar 0,167673552 mg/ml. Aktivitas enzim spesifik tertinggi terjadi pada hari ke-11 dengan nilai 0,0538 U/mg, sedangkan aktivitas enzim spesifik terendah tidak memiliki aktivitas atausama dengan nilai 0 U/mg.Â Kata kunci : penapisan, bakteri selulolitik, enzim selulase

PENINGKATAN PENGETAHUAN MASYARAKAT TENTANG TANAMAN TRANSGENIK MELALUI EDUKASI ONLINE Wahyuni, Febriana Dwi; Novianti, Titta; Saraswati, Henny; Seprianto, Seprianto
Jurnal Pengabdian Masyarakat AbdiMas Vol 8, No 01 (2021): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v8i01.4785

Transgenic plants are genetically modified plants made by inserting one or a number of genes from other organisms, with the aim of obtaining superior and desirable new traits, such as resistance to drought stress, resistance to pests, resistance to herbicides. So far, there are still many people who are worried about the safety of transgenic plants that have been successfully marketed. Therefore, we strive to provide information based on existing references to increase public understanding of GM crops. The educational materials provided consisted of an introduction to plant breeding techniques and the purpose of genetic transformation in plants, gene cloning techniques and the transformation of target genes into plant genomes to produce transgenic plants and molecular techniques used to select transgenic plants which were delivered in the form of online seminars. via zoom and live streaming on youtube. The flow of activities is divided into two stages, namely material presentation and discussion. This activity is expected to increase knowledge about assembling techniques for transgenic plants as an effort to educate the public regarding the safety of transgenic plants. Keywords: biotechnology, cloning, GMOs, transgenic plants

PENGENALAN BIOTEKNOLOGI DAN METODE KULTUR JARINGAN SEBAGAI UPAYA PENINGKATAN WAWASAN SISWA DI SMA YAYASAN PERSIAPAN GENERASI BARU Wahyuni, Febriana Dwi; Novianti, Titta; Saraswati, Henny; Seprianto, Seprianto
Jurnal Pengabdian Masyarakat AbdiMas Vol 6, No 3 (2020): Jurnal Pengabdian Masyarakat Abdimas
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v6i3.3333

AbstractHigh school students is a level of secondary school education that will enter a higher level of education in tertiary institutions. Of course, a lot of preparation must be done as a provision after graduating, both knowledge and skills. This community service activity is one of the first steps to introduce and enhance secondary school students' insights about biotechnology. Biotechnology is a relatively new study program in Indonesia. Biotechnology has many focus areas that can be studied, including tissue culture and microbiology. The community service activity aims to provide knowledge not only to students, but also to teachers in the Senior High School Foundation Preparation Foundation regarding tissue culture and microbiology techniques. The method used for the delivery of the material is the lecture and practicum methods. Mastery of tissue culture and microbiology is expected to make students as creative individuals in creating a product that is related to these two focus areas.Ã‚Â Keywords: biotechnology, tissue culture, microbiologyÃ‚Â AbstrakSiswa SMA merupakan tingkatan dari pendidikan sekolah menengah yang akan memasuki tingkatan pendidikan yang lebih tinggi di perguruan tinggi. Tentunya banyak persiapan yang harus dilakukan sebagai bekal setelah lulus nantinya, baik ilmu maupun keterampilan. Kegiatan pengabdian kepada masyarakat ini merupakan Ã‚Â salah satu langkah awal untuk memperkenalkan dan meningkatkan wawasan siswa Sekolah Menengah tentang bioteknologi. Bioteknologi merupakan salah satu program studi yang terhitung baru di Indonesia. Bioteknologi mempunyai banyak bidang fokus yang bisa dipelajari, diantaranya yaitu kultur jaringan dan mikrobiologi. Adapun kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan pengetahuan tidak hanya kepada siswa, tetapi juga kepada guru di lingkungan SMA Yayasan Persiapan Generasi Baru mengenai teknik-tekniik kultur jaringan dan mikrobiologi. Adapun metode yang digunakan untuk penyampaian materi tersebut adalah metode ceramah dan praktikum. Penguasaan tentang kultur jaringan dan mikrobiologi ini diharapkan dapat menjadikan siswa sebagai individu yang kreatif dalam menciptakan suatu produk yang berkaitan dengan kedua bidang fokus tersebut.Ã‚Â Kata Kunci : bioteknologi, kultur jaringan, mikrobiologi

PKM PENINGKATAN MUTU PANGAN LOKAL BERBASIS PANGAN FERMENTASI DI PULAU PAYUNG, KEPULAUAN SERIBU Seprianto, Seprianto; wahyuni, Febriana Dwi
Jurnal Pengabdian Masyarakat AbdiMas Vol 6, No 2 (2020): JURNAL PENGABDIAN MASYARAKAT ABDIMAS
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47007/abd.v6i2.3186

AbstractThe Thousand Islands Region is administratively included in DKI Jakarta Province but has different characteristics from other DKI Jakarta areas. Payung Island is one of the islands in the Thousand Islands which is a marine tourism destination that is quite crowded with tourists. The diversity of processed products, especially fish-based products, is still little developed on this island, so there are not many choices for tourists. The purpose of this activity is to develop diversified food products by improving the quality of fermented-based local food. Through the cooperation of UMKMBarakuda Payung Island partners, the implementation of community service is going well. The method of implementation is by presentation and direct practice such as making peda fish, while other processed products such as bekasang and fish sauce were only a video documentary. A total of 13 members of the Barakuda group participated in this training. Processed products from fish have not been developed much by UMKM Barakuda, Payung Island. This activity also collaborates with the Product Design Team, so that it can provide an overview of the product from manufacturing to the packaging stage. It is expected that the implementation of this activity will increase the role of the community to be creative, innovative, independent, and entrepreneurial so that they can actively participate in regional and national development and become an attraction for tourists to visit the Thousand Islands.Ã‚Â Keywords :Payung island, local food, fermentation foodÃ‚Â AbstrakWilayah Kepulauan Seribu secara administratif masuk ke dalam Propinsi DKI Jakarta, namun memilikiÃ‚Â karakteristik yang berbeda dengan wilayah DKI Jakarta lainnya. Pulau Payung merupakan salah satu pulau di Kepulauan Seribu yang menjadi destinasi wisata bahari yang cukup ramai dikunjungi oleh wisatawan.Keberagaman produk olahan terutama berbahan dasar ikan masih sedikit dikembangkan di pulau ini, sehingga tidak banyak pilihan bagi wisatawan.Tujuan kegiatan ini adalah untuk pengembangan diversifikasi produk pangan dengan meningkatkan mutu pangan lokal berbasis fermentasi.Melalui kerjasama mitra UMKM Barakuda Pulau Payung, pelaksanaan pengabdian masyarakat ini berjalan dengan baik.Metode pelaksanaannya dengan presentasi dan praktek langsung seperti pembuatan ikan peda, sedangkan produk olahan lainnya seperti bekasang dan kecap ikan hanya berupa video dukumenter.Sebanyak 13 anggota kelompok UMKM Barakuda mengikuti pelatihan ini.Produk olahan dari ikan tersebut belum banyak dikembangankan oleh UMKM Barakuda Pulau Payung.Kegiatan ini juga berkalaborasi dengan Tim Desain Produk, sehingga dapat memberikan gambaran produk mulai dari pembuatan sampai pada tahap pengemasannya. Diharapkan dari pelaksanaan kegiatan ini akan meningkatan peranan masyarakat untuk kreatif, inovatif, mandiri, sertaÃ‚Â berwirausaha sehingga mampu berpartisipasi aktif dalam pembangunan daerah dan nasional dan menjadi daya tarik wisatawan untuk berkunjung ke Kepulauan Seribu.Ã‚Â Kata kunci:Pulau payung, pangan lokal, pangan fermentasi

ISOLASI DAN PENAPISAN BAKTERI SELULOLITIK DARI BERBAGAI JENIS TANAH SEBAGAI PENGHASIL ENZIM SELULASE Seprianto, Seprianto
Indonesian Journal of Biotechnology and Biodiversity Vol 1, No 2 (2017): Indonesian Journal of Biotechnology and Biodiversity
Publisher : Universitas Esa Unggul

Show Abstract | Download Original | Original Source | Check in Google Scholar

AbstractCellulose is an enzyme that hydrolyzes Î²-1,4-glycosidic bonds on cellulose molecules to produce glucose. The utilization of cellulase enzymes is currently increasing in the food, paper , detergent and agricultural industries. This research was aimed at obtaining cellulolytic bacterial isolates capable of producing cellulose enzyme where enzyme activity was measured daily by DNS method and to add bacterial isolate collection which will be used for further research. Screening of cellulolytic bacteria from soil led to detection or one potential isolate, designated as 6.2 showed highest activity among others which occurred on the 11th day with activity value of 0,0189 Î¼mol/minute and the lowest activity of cellulase enzyme was not observed its activity, while the highest dissolved protein content occurred on the sixth day with activity of 0,408240784 mg/ml and the lowest protein content on day 2th of 0,167673552 mg / ml. The highest activity of specific enzyme occurred on the 11th day with a value of 0,0538 U/mg, while the lowest activity of specific enzyme was not activity with value of 0 U/mg.Â Keywords : screening, cellulolytic bacteria, celluloseÂ AbstrakEnzim selulase merupakan enzim yang dapat menghidrolisis ikatan Î²-1,4-glikosidik pada molekul selulosa sehingga menghasilkan glukosa. Pemanfaatan enzim selulase saat ini semakin meningkat dalam dunia industri makanan, industri kertas, industri detergen dan industri pertanian. Penelitian ini bertujuan untuk mendapatkan spesies bakteri selulolitik yang potensial menghasilkan enzim selulase dan melihat aktivitas enzim dengan metode pengukuran DNS serta menambah koleksi isolat bakteri dari biakan murni yang akan digunakan untuk penelitian selanjutnya. Dari hasil penelitian penapisan bakteri selulolitik yang diisolasi dari berbagai jenis tanah didapatkan satu isolat yang potensial yaitu Isolat 6.2 menunjukan aktivitas lebih baik diantara yang lainnya dengan aktivitas enzim ektrak kasar tertinggi terjadi pada hari ke-11dengan nilai 0,0189 Âµmol/menit dan aktivitas enzim selulase terendah tidak teramati aktivitas enzimnya atau sama dengan nilai 0 Âµmol/menit, sedangkan kadar protein terlarut tertinggi terjadi pada hari ke-6 dengan nilai 0,408240784 mg/ml dan kadar protein terendah pada hari ke-2 sebesar 0,167673552 mg/ml. Aktivitas enzim spesifik tertinggi terjadi pada hari ke-11 dengan nilai 0,0538 U/mg, sedangkan aktivitas enzim spesifik terendah tidak memiliki aktivitas atausama dengan nilai 0 U/mg.Â Kata kunci : penapisan, bakteri selulolitik, enzim selulase

Isolasi dan Identifikasi Bakteri Probiotik Dari Usus Udang Windu (Penaeus monodon) Berdasarkan Sekuens Gen 16S rDNA Seprianto S; Feliatra F; Titania T Nugroho
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 2 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i2.3943

Probiotics are living microorganisms that have beneficial effect on the host by improving the balance of bacteria in the intestines. This research was aimed to determine the diversity of bacteria that live in digestion of the shrimp and molecular characteristics along with to observe phylogenetic relationships among the bacteria identified based on sequence16S rDNA. The bacteria were isolated from intestine of the Tiger prawn (Penaeus monodon) obtained from fishpond at BBPBAP Jepara. The analyzed result of 16S rDNA shown that three bacterial species were potential as probiotic. These bacteria grow well at pH 2 and this indicates one of probiotic bacteria characteristic. Among the three isolates, two isolates i.e. SP2 and SU isolates were phylogenetically closely related to Bacillus bataviensis strain CCGE2059 (EU867382.1) with 97% homology. While the SWU Isolate is most likely a new species of Caulobacter sp. (AJ227775.1). The SWU isolates were phylogenetically one ancestor with the Chromobacterium violaceum.

Candida antarctica Lipase B Synthetic Gene: A Bioinformatics Analysis Febriana Dwi Wahyuni; seprianto Seprianto
Bioscience Vol 2, No 2 (2018): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Enzyme exploration is important to support the development of biotechnology. To facilitate microbial discovery, it can be done synthetically using bioinformatics methods. Candida antarctica lipase B (CALB) is one of enzyme derived from microorganisms and has been applied as a biocatalyst in several industry. The methods used in this study are the analysis of gene structure using NCBI, analysis of protein CALB sequence using Uniprot, analysis of 3D protein structure using Swiss model, analysis primary design using primer3 and analysis of restriction sites using snapgene. The construction of synthetic calB gene obtained based on the results of the CalB gene sequence in geneBank using NCBI with access number Z30645.1. CalB gene wildtype is modified by adding several restriction enzyme (XhoI, XbaI, ClaI, and BglI), 6 Histags, and 2x stops codon and produce 1083 base pairs. CALB protein has 342 amino acids. Based on 3D structure, CALB protein has three molecules in common with one homotrimer ring that will encircle the double helix DNA. Primers used for calB gene amplification are CALB forward 5'-TCCCCAGTATCAGGTCCAAG-3 ' and CALB reverse 5'-GACACCTGAGGCTGAACGAT-3'.

Cloning of a Transglutaminase Gene from Streptomyces thioluteus TTA 02 SDS 14 Seprianto Seprianto; Dewi Seswita Zilda; Yusro Nuri Fawzya; Suharsono Suharsono; Puspita Lisdiyanti; Agustinus Robert Uria
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 11, No 1 (2016): May 2016
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v11i1.189

Microbial Transglutaminase (MTGase, EC 2.3.2.13) is an enzyme that catalyzes the transfer of acyl group. Many microbial strains that produce MTGase belong to Streptomyces members. This research was aimed at cloning of a MTGase gene. PCR–based screening of ten MTGase-producing streptomyces isolates from soil in West Nusa Tenggara led to detection of one potential isolate, designated as TTA 02 SDS 14. The partial MTGase-encoding gene (470 bp) was amplified by PCR and sequenced. The sequence result indicate its similarity of 93 % with that of Streptomyces cinnamoneus. The 16S rRNA gene analysis showed its identity as Streptomyces thioleteus. Fosmid-based construction of a genomic library from the isolate and subsequent screening led to the isolation of a ~40-Kb fosmid harboring a MTGase gene.

MUTATION DETECTION OF MULTIDRUG-RESISTANT TUBERCULOSIS BY RT-PCR METHOD AS THE DIAGNOSTIC TOOL OF MDR-TB Titta Novianti; alfero Putra Iryanto; feby feby; callista marsya; putri mega utami; febriana dwi wahyuni; henny saraswati; seprianto; adri nora; roaslein putri; nie nie; sabar pambudi
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 1 (2023): Juni 2023
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v10i1.5653

Eight percent of tuberculosis (TB) cases worldwide are resistant to rifampicin, with mutations occurring in the rpoB and katG genes. It is necessary to develop a specific multidrug-resistant (MDR) diagnostic technique using the RT-PCR method in Indonesia to aid in rapid and accurate diagnosis. In-silico testing using SnapGene software resulted in the design of DNA primers for the katG and rpoB genes, plasmids, and specific probes. This study employed a cross-sectional design using 30 non-MDR-TB and MDR-TB samples from RSUD Sitanala, Tangerang Banten, which were tested for amplification of the katG and rpoB genes using Sybr green RT-PCR. Validity testing was conducted using specific probes for the katG and rpoB genes. The amplification results showed that MDR-TB samples and MDR-TB plasmids required a longer time compared to non-MDR-TB samples and non-MDR-TB plasmids. The Quantification Cycle (Cq) value in non-MDR-TB samples was lower than the Cq value in MDR-TB samples. A t-test revealed a significant difference in Cq values of the rpoB and katG genes between MDR-TB and non-MDR-TB patients (p-value < 0.005). These differences in Cq values indicate that the findings of this study can serve as an initial reference for the development of an RT-PCR-based diagnostic kit for MDR-TB.

Optimasi Volume Kit Da An Gene Untuk Deteksi SARS-CoV-2 dengan Real Time RT-PCR Seprianto Seprianto; Muhammad Arreza; Titta Novianti; Febriana Dwi Wahyuni; Oktaviani Naulita Turnip; Roaslein Putri; Henny Saraswati
BIOEDUSCIENCE Vol 6 No 2 (2022): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/j.bes/628595

Background: SARS-CoV-2 is a new type of coronavirus of the genus Betacoronavirus and the family Coronaviridae that causes a respiratory disease called COVID-19. The virus has a sheath and genetic material in the form of single-chain RNA. The genome structure of this virus is divided into two types, namely genes that encode non-structural proteins consisting of the ORF1a / ORF1b gene and genes that encode structural proteins consisting of spike glycoprotein (S), envelope (E), membrane glycoprotein (M), and nucleocapsid protein (N). Methods: The method of detecting SARS-CoV-2 with real time RT-PCR is the most recommended method because it has high specificity and accuracy. The specificity of a method is necessary to be able to specifically recognize the pathogen that causes the disease. Real time RT-PCR requires sampling with a swab on the oropharynx or nasopharynx to be examined in the laboratory which later the presence of viral RNA becomes a molecule that is assessed for diagnosis results. In this study, volume optimization was carried out on the Da An Gene kit used for the detection of SARS-CoV-2 with Reverse Transcription Polymerase Chain Reaction (Real time RT-PCR) with the aim of saving the use of reagents from available kits but with amplification results remaining optimal and accurate. Results: There were three SARS-CoV-2 RNA samples used consisting of N62, N63, and N79 samples and three types of total volume used were 20 Î¼l, 15 Î¼l, and 10 Î¼l. The results of this study showed that the three positive samples contained SARS-CoV-2 with a Cq value of < 40. Conclusion: A volume of 20 Î¼l is the optimal volume, which is more efficient than the manufacturer's recommended volume of 25 ul.

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