Indonesian Food and Nutrition Progress
Vol 12, No 1 (2005)

Isolation and Identification of Antiradical and Anti-photooxidant Component of Aloe vera chinensis

Yohana S. Kusuma Dewi (Tanjungpura University)
Tranggono Tranggono (Gadjah Mada University)
Sri Raharjo (Gadjah Mada University)
Puji Hastuti (Gadjah Mada University)



Article Info

Publish Date
20 May 2014

Abstract

A potent antiradical and antiphotooxidant compound from Aloe vera chinensis was investigated. The results indicated that the methanolic extracts produced stronger antiradical and antiphotoaxidant activity also gave higher yields of extract than other organic solvents. Silica gel column separated methanolic extracts into five fractions. Of the five fractions, fraction II possessed significant antiradical activity (70.2 % inhibition on free radical DPPH) and its showed significant antiphotooxidant activity (60.1 % inhibition on hydroperoxide formation). Subsequently, fraction II was separated into eight subfractions by silica gel column. The sub fraction II-b exhibited stronger antiradical and antiphotooxidant activity than others and showed a single spot in thin layer chromatography Silica gel 60 F254 plates. On the basis of mass spectrophotometry in combination with reversed phased, high performance chromatography, this compound has been identified as 1,8 dihydroxy-3- hydroxymethylanthraquinone (Aloe emodin).

Copyrights © 2005






Journal Info

Abbrev

ifnp

Publisher

Subject

Agriculture, Biological Sciences & Forestry

Description

Indonesian Food and Nutrition Progress, is a primarily online, a peer-reviewed journal in food technology and nutrition. All papers should focus on the novelty of the research carried out. It is published by Indonesian Association of Food Technologists in collaboration with Faculty of Agricultural ...