HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of Kaempferia galanga from Related Species

Cahya Septyanti (Department of Chemistry, Faculty of Mathematic and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Kimia Building, Tanjung Street, Bogor 16680)
Irmanida Batubara (Department of Chemistry, Faculty of Mathematic and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Kimia Building, Tanjung Street, Bogor 16680 Biopharmaca Research Center-Research and Community Empowerment Institute, Bogor Agricultur)
Mohamad Rafi (Biopharmaca Research Center-Research and Community Empowerment Institute, Bogor Agricultural University, Bogor 16128)

Indonesian Journal of Chemistry
Vol 16, No 3 (2016)

Fingerprint analysis using high performance liquid chromatography (HPLC) has been developed for authentication of Kaempferia galanga from related species, such as Kaempferia pandurata and K. rotunda. By comparing the fingerprint chromatograms of K. galanga, K. pandurata and K. rotunda, we could identify K. galanga samples and detect adulteration of K. galanga from K. pandurata and K. rotunda by using their marker peaks. We also combined HPLC fingerprint with chemometrics for discrimination the three species and also for authentication of K. galanga. All the three species and K. galanga adulterated with K. pandurata and K. rotunda were discriminated successfully by using principal component analysis (PCA) and discriminant analysis (DA). This result indicates that HPLC fingerprint analysis in combination with PCA (PC1 = 30.06% and PC2 = 34.74%) and DA (DF1 = 94.59% and DF2 = 3.32%) could be used for authentication of K. galanga samples from the related species.