Indonesian Journal of Chemistry
Vol 15, No 3 (2015)

Polyaniline-Invertase-Gold Nanoparticles Modified Gold Electrode for Sucrose Detection

Fitriyana Fitriyana (Chemical Engineering Department, Politeknik Negeri Samarinda, Jl. Dr. Cipto Mangunkusumo, Samarinda 75131)
Fredy Kurniawan (Department of Chemistry, Sepuluh Nopember Institute of Technology (ITS), Jl. Arief Rahman Hakim, Sukolilo, Surabaya 60111)



Article Info

Publish Date
12 Nov 2015

Abstract

Sucrose sensor has been made by deposited the active materials on the surface of gold electrode. The active materials, i.e. polyaniline (PANI), invertase and gold nanoparticles, were deposited step by step. Aniline polymerization were conducted electrochemically at potential -500 to 1000 mV using voltammetry method with sweep rate 50 mV/s for 20 cycles in HCl solution pH 1.5. The modified electrode obtained was immersed in invertase 1 M phosphate buffer solution pH 6. The invertase trapping in polyaniline was performed using the same condition as aniline polymerization. Then, gold nanoparticles were deposited on the polyaniline-invertase modified gold electrode using Layer by Layer (LbL) technique. The polyaniline-invertase-gold nanoparticles modified gold electrode obtained was used to measure sucrose solution. Electrochemical signal of polyaniline (PANI)-invertase-gold nanoparticles modified gold electrode is increase with sucrose concentration. The sensitivity and detection limit of the electrode are 0.4657 µA mm-2 mM-1 and 9 µM, respectively. No electrochemical interference signals from fructose and glucose have been observed in the sucrose measurement.

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Journal Info

Abbrev

ijc

Publisher

Subject

Chemical Engineering, Chemistry & Bioengineering Chemistry

Description

Indonesian Journal of Chemistry is an International, peer-reviewed, open access journal that publishes original research articles, review articles, as well as short communication in all areas of chemistry including applied chemistry. The journal is accredited by The Ministry of Research, Technology ...