Major Anthocyanin Pigments in the Ficus padana Fruits: HPLC-DAD-ESI-MS Identification and Antioxidant Activity

Daimon Syukri (Department of Agricultural Product Technology, Andalas University, Limau Manis, Padang 25163, West Sumatra)
Djaswir Darwis (Department of Chemistry, Andalas University, Limau Manis, Padang 25163, West Sumatra)
Adlis Santoni (Department of Chemistry, Andalas University, Limau Manis, Padang 25163, West Sumatra)

Indonesian Journal of Chemistry
Vol 14, No 3 (2014)

The anthocyanins in Ficus padana were extracted and identified by using high-performance liquid chromatography/diode array detection and electrospray ionization/mass spectrometry (HPLC-DAD-ESI-MS). The individual anthocyanins were identified by comparing between their mass spectral data and published data. The first compound (peak 1) was identify as a pelargonidin 3-(6”-p-coumarylglucoside)-5-(4”’-Malonylglucoside) and the second compound was identify as a pelargonidin 3-(6’’-Malonylglucoside). The second one is a major compound that taking up to 91% of the total anthocyanin content in F. padana extract. The antioxidant activity was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays and results showed that extract possessed high antioxidant capacity.