IMMOBILIZATION OF PAPAIN ON CHITOSAN

Sari Edi Cahyaningrum (Chemistry Department, Faculty of Mathematics and Natural Sciences Surabaya State University, Jl. Ketintang, Surabaya 60231)
Narsito Narsito (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281)
Sri Juari Santoso (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Sekip Utara, Yogyakarta 55281)
Rudiana Agustini (Chemistry Department, Faculty of Mathematics and Natural Sciences Surabaya State University, Jl. Ketintang, Surabaya 60231)

Indonesian Journal of Chemistry
Vol 8, No 3 (2008)

In this study, papain was immobilized on chitosan with Mg(II) cosslinked agent. Studies on free and immobilized papain systems for determination of optimum pH, optimum temperatur, thermal stability and reusability were carried out. The results showed that free papain had optimum pH 6.5 and optimum temperature 55 °C while the immobile papain hadoptimum pH 8 and optimum temperature 80 °C. The thermal stability of the immobilized papain, relative to that of the free papain, was markedly increased. The immobilized papain can be reused for at least six times.