STRUCTURE ELUCIDATION OF BIOACTIVE ALKALOID COMPOUNDS ISOLATED FROM SPONGE Petrosia sp COLLECTED FROM BUNAKEN BAY MENADO

Gemini Alam (Department of Pharmacy, Faculty of Mathematic and Natural Sciences, Hasanuddin University, Makassar)
Puji Astuti (Pharmaceutical Biology Department, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta)
Subagus Wahyuono (Pharmaceutical Biology Department, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta)
Dinar Sari (Biology Department, Faculty of Mathematic and Natural Sciences, Sebelas Maret University, Surakarta)
Mark T Hamman (Department of Pharmacognosy, Faculty of Pharmacy, Mississippi University)

Indonesian Journal of Chemistry
Vol 5, No 2 (2005)

Two alkaloid compounds have been isolated from sponge Petrosia sp collected from Bunaken Bay Menado. These compounds have been found to inhibit several human cancer cell proliferation in vitro. Structure elucidation is needed to further characterize these bioactive compounds. The structure is determined by analyzing Ultra Violet (UV), Infra Red (IR), Mass Spectra (MS) and Nuclear Magnetic Resonance (NMR) spectra. Based on spectroscopic data, Thin Layer Chromatography (TLC) co-chromatograph with authentic samples and comparison with reported data the two compounds were identified as Manzamine A and 8-OH Manzamine A, respectively.