Nitric oxide (NO) level of the follicular fluid in endometriosis patients

Cahyono Hadi (Department of Obstetrics and Gynecology Faculty of Medicine Diponegoro University, Indonesia)
Djaswadi Dasuki (Department of Obstetrics and Gynecology Faculty of Medicine Gadjah Mada University)
Ahmad Hamim Sadewa (Department of Biochemistry Gadjah Mada University)
Indwiani Astuti (Department of Pharmacotherapy, Molecular Biology Faculty of Medicine Gadjah Mada University)
Awal Prasetyo (Diponegoro University)

Majalah Obstetri dan Ginekologi
Vol. 26 No. 1 (2018): April

Objectives: To ascertain the nitric oxide (NO) level of the follicular fluid of endometriosis sufferers compared with that of the non-endometriosis patients.Materials and Methods: Follicular fluid was taken from 64 women, but only 54 continued with further laboratory test. Women with endometriosis had their follicular fluid taken during laparoscopy, while the follicular fluid from non-endometriosis women was taken during tubectomy. Nitric Oxide level was analyzed using the PCR-RFLP method. Data were analyzed using software STATA Intercooled Version 11.Results: NO level in endometriosis is higher than that in non-endometriosis and has a significant relationship with all the variables (age, parity, dysmenorrhea, dyspareunia, types of endometriosis, and grade of endometriosis).Conclusion: The NO level in endometriosis is higher than that in non-endometriosis. The distribution of the polymorphism of allele GG is greater than that in non-endometriosis. The existence of polymorphism GG caused a higher eNOS, increasing NO level, causing a decrease in fertility which derived from the degenera-tive oocyte.