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All Journal MEDIA MEDIKA INDONESIANA Media Kesehatan Masyarakat Indonesia Nurse Media Journal of Nursing Jurnal Kedokteran Diponegoro Jurnal NERS Majalah Obstetri dan Ginekologi The Indonesian Biomedical Journal Biomedical Journal of Indonesia Universa Medicina SPEKTRA: Jurnal Kajian Pendidikan Sains Jurnal Penelitian Pendidikan IPA (JPPIPA) Belitung Nursing Journal IJEMS (Indonesian Journal of Environmental Management and Sustainability) CARING (Center of Publication in Midwifery and Nursing) : Jurnal Publikasi Penelitian Kebidanan dan Keperawatan Media Medika Muda Majalah Patologi Indonesia Jurnal Biomedika dan Kesehatan Jurnal Smart Keperawatan Bioscientia Medicina : Journal of Biomedicine and Translational Research Medica Hospitalia Jurnal Ekonomi Indonesia Bioscientia Medicina : Journal of Biomedicine and Translational Research Life Science Journal of Health and Nutrition Research Berita Kedokteran Masyarakat
Awal Prasetyo
Departemen Patologi Anatomik Fakultas Kedokteran Universitas Diponegoro
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Dentistry Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health

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Sensitivity and Specificity of the Comfort Scale to Assess Pain in Ventilated Critically Ill Adult Patients in Intensive Care Unit Wahyuningsih, Indah S; Prasetyo, Awal; Utami, Reni Sulung
Nurse Media Journal of Nursing Vol 7, No 1 (2017): (JUNE 2017)
Publisher : Department of Nursing, Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.895 KB) | DOI: 10.14710/nmjn.v7i1.15126

Abstract

Background: Pain is a common phenomenon experienced by ventilated and critically ill adult patients. It is urgent to measure the pain among these patients since they are unable to report their pain verbally. Comfort Scale is one of the instruments used to measure pain in adult patients. The scale is used to measure pain among children patients with fairly high sensitivity and specificity.Purpose: This study aimed to examine the sensitivity and specificity of the Comfort Scale to measure pain in the ventilated critically ill adult patients in the ICU.Methods: This study employed a cross-sectional design with 66 ventilated adult patients in the ICUs of two hospitals in Semarang. The pain assessment was administered to the patients in 2 duplo periods by two observers comparing with the Comfort Scale and Critical Care Pain Observational Tool (CPOT) as a gold standard instrument during the pre and post positioning procedures. The data were analyzed using the receiver operating curve (ROC).Result: The results showed that in the pre-positioning procedure, the Comfort Scale had the sensitivity value of 69% and the specificity value of 81%. Meanwhile, in the post-positioning procedure, the values were decreasing (the sensitivity of 45%, the specificity of 67%). This indicated that the sensitivity value of the comfort scale decreased and could be interpreted that the ability of the instrument to detect pain remained low. Meanwhile, the decrease of the specificity value of the instrument between the pre and post administration was not far different, so it could be interpreted that the instrument can correctly identify the patient without pain.Conclusion: The Comfort Scale had a lower value of sensitivity and specificity in the post-positioning than that in the pre-positioning procedure. It is recommended that further studies should focus on the relationship between sedation and pain by using instruments of pain studies for adult patients (CPOT). Additionally, the hospital policy makers, that is Pain Task Force is expected to give education and training through workshops and seminars about the nurse skills in pain management on critical areas as part of the multidisciplinary team.
PENGARUH EKSTRAK KULIT BUAH NAGA MERAH TERHADAP KADAR AST DAN ALT DARAH TIKUS SETELAH AKTIFITAS FISIK MAKSIMAL Siahaan, Johanna; Tjahjono, Kusmiyati; Prasetyo, Awal
DIPONEGORO MEDICAL JOURNAL (JURNAL KEDOKTERAN DIPONEGORO) Vol 8, No 3 (2019): JURNAL KEDOKTERAN DIPONEGORO
Publisher : Faculty of Medicine, Diponegoro University, Semarang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (313.079 KB) | DOI: 10.14710/dmj.v8i3.24493

Abstract

Latar Belakang: Aktivitas fisik maksimal dapat menyebabkan terjadinya stres oksidatif, yang  kemudian merusak sel hepar. Salah satu penanda adanya kerusakan sel hepar adalah kadar AST dan ALT. Pemberian ekstrak kulit buah naga merah dapat berfungsi sebagai anti stres oksidatif. Kulit buah naga merah merupakan salah sumber antioksidan karena mengandung antosianin, betalain, vitamin C dan E. Tujuan: Mengetahui pengaruh ekstrak kulit buah naga merah terhadap kadar AST dan ALT tikus setelah aktivitas fisik maksimal. Metode: Penelitian true experimental dengan desain post test only control group design pada tikus jantan. Sampel penelitian sebanyak 18 tikus dibagi 3 kelompok dengan perlakuan yang berbeda. Kelompok K1 diberikan pakan standar dan aktifitas fisik maksimal, K2 diberikan pakan standar dan ekstrak kulit buah naga merah, dan P diberikan pakan standar, ekstrak kulit buah naga merah dan aktivitas fisik maksimal.  Hasil: Uji beda Post Hoc Bonferroni pada ALT berdasarkan kelompok perlakuan didapatkan bahwa antara perlakuan K1 terhadap K2 didapatkan nilai P = 1,000, K1 terhadap P nilai P = 0,031 dan K2 terhadap P nilai P= 0,093, sehingga dapat disimpulkan antara kelompok K1 terhadap K2 tidak berbeda bermakna (P > 0,05), begitu juga antara kelompok K2 terhadap P tidak berbeda bermakna (P> 0,05), sedangkan antara kelompok K1 terhadap P terdapat perbedaan bermakna (P< 0,05).Tidak terdapat penurunan kadar AST dan ALT pada semua kelompok . Terdapat peningkatan kadar AST dan ALT pada K1, K2, P. Kesimpulan: Ektrak kulit buah naga merah tidak yang diberikan sebagai antioksidan eksogen tidak dapat menurunkan kadar AST dan ALT darah tikus secara  bermakna setelah aktivitas fisik maksimal.Kata Kunci: Ekstrak kulit buah naga merah, AST, ALT, aktivitas fisik maksimal
Pengaruh Intervensi Mindfulness Terhadap Intensitas Kecemasan pada Pasien Kanker Nasopharal dengan Radiasi Jumatul Laely, Anna; Prasetyo, Awal; Bagus Ropyanto, Chandra
Journal Center of Research Publication in Midwifery and Nursing Vol 1 No 2 (2017): Journal Center of Research Publication in Midwifery and Nursing
Publisher : STIKES Bina Usada Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36474/caring.v1i2.2

Abstract

Kecemasan yang terjadi pada pasien kanker nasofaring pada masa radioterapi terutama disebabkan karena efek dari radiasi tersebut. Kecemasan pada tahap pengobatan menjadi sangat penting untuk diatasi karena dapat menyebabkan komorbiditas, trauma psikologis dan peningkatan mortalitas. Mindfulness merupakan psikoterapi yang efektif digunakan untuk mengurangi kecemasan pada pasien. Penelitian ini bertujuan untuk mengetahui pengaruh intervensi mindfulness terhadap intensitas kecemasan pada pasien kanker nasofaring yang sedang menjalani pengobatan radiasi. Metode penelitian yang digunakan pada penelitian ini adalah quasy eksperiment pre-post control trial design. Tigapuluh tiga responden dipilh dengan consecutive sampling dan terbagi dalam dua kelompok yaitu kelompok perlakuan dan kelompok kontrol. Pengukuran intensitas kecemasan dilakukan sebelum dan sesudah diberikan mindfulness selama 6 sesi menggunakan instrument Hamilton rating scale for anxiety (HAM-A). Uji hipotesis dilakukan untuk melihat pengaruh mindfulness dengan membedakan hasil sebelum dan sesudah dilakukannya intervensi pada kedua kelompok. Hasil Penelitian menunjukkan terjadi penurunan rata-rata tingkat kecemasan pada kelompok perlakuan setelah diberikan intervensi mindfulness dibandingkan dengan kelompok kontrol. Terdapat perbedaan signifikan tingkat kecemasan sebelum dan sesudah dilakukan mindfulness pada kelompok perlakuan dengan nilai P =0,001 (P<0,005).
The Effect of Hypnotherapy on Anticipatory Nausea in Head and Neck Cancer Patients Undergoing Chemotherapy Hamdani, Dadi; Prasetyo, Awal; Anggorowati, Anggorowati
Nurse Media Journal of Nursing Vol 10, No 3 (2020): (December 2020)
Publisher : Department of Nursing, Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/nmjn.v10i3.26027

Abstract

Background: One of the chemotherapy side effects on head and neck cancer patients is anticipatory nausea. However, the anticipatory nausea problem has not been properly resolved. Nausea might be psychologically reduced by hypnotherapy. There only have been very few studies conducted to examine the effects of hypnotherapy in alleviating anticipatory nausea.Purpose: This study aimed to determine the effect of hypnotherapy using Hanung induction technique on anticipatory nausea in head and neck cancer patients undergoing chemotherapy.Methods: This research employed a pre-post test of quasi-experiment with control group design. Consecutive sampling technique was used to obtain 64 subjects who met inclusion and exclusion criteria and were equally divided into the intervention and control groups. Hypnotherapy as the intervention was carried out in two sessions, each of which lasted for 20 minutes, with a week distance between sessions. The data were collected using a visual analog scale (VAS), which was used twice to measure anticipatory nausea and analyzed using the paired and independent-sample t-test.Result: The results showed that the mean score of anticipatory nausea in the intervention group reduced from 7.6±1.4 to 2.3 ±1.2 after hypnotherapy, while the mean in the control group increased from 6.4±1.6 to 6.7±1.4. There was a significant difference in the score of anticipatory nausea after the implementation of hypnotherapy between the intervention and the control group (p<0.001).Conclusion: The study concluded that hypnotherapy is effective in reducing the intensity of anticipatory nausea in head and neck cancer patients undergoing chemotherapy. Therefore, hypnotherapy can be applied by oncology nurses as an intervention in treating anticipatory nausea.
Nephrolepis exaltata - Hibiscus rosa-sinensis combination herbal mask decreases levels of reactive oxygen species in nasal wash Prasetyo, Awal; Ariani, Resti; Antari, Arlita Leniseptaria; Sadhana, Udadi; Miranti, Ika Pawitra; Kristina, Tri Nur
Universa Medicina Vol. 39 No. 3 (2020)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2020.v39.184-191

Abstract

BackgroundPoor air and mask quality in the work environment increase the risks of inflammation, reactive oxygen species (ROS) production, ongoing oxidative stress, and superoxide dismutase 3 (SOD3) levels in the airways. The study aimed to investigate the effect of the Nephrolepis exaltata - Hibiscus rosa-sinensis (NE-HRS) combination herbal mask on the airways, as measured by ROS and SOD3 levels in the nasal wash of textile industry workers.MethodsThis was a pre- and post-test randomized controlled trial, consisting of 30 subjects with ³5-year working time, conducted for eight weeks and monitored every ten days. Subjects were randomized into 3 groups, regular surgical mask group (n=10), NE-HRS combination herbal mask group (n=10) and factory mask group (n=10). SOD3 and ROS levels were determined using ELISA. Data were analyzed with One-way ANOVA and multiple comparison tests.Results ROS levels decreased significantly in all groups (p=0.004). Regular surgical masks decreased ROS levels by 67.90 ± 50.66 pg/mL, NE-HRS combination herbal masks by 49.91 ± 45.06 pg/mL, and factory masks by 193.96 ± 128.36 pg/ml. SOD3 levels also decreased but not significantly (p=0.053). Differences in ROS levels between surgical mask and NE-HRS combination herbal mask was not significant (p=0.796). ConclusionHerbal masks laminated with NE-HRS combination significantly reduced ROS levels among textile industry workers. NE-HRS combination herbal masks have a similar protective effect to the regular surgical mask.
Metode Penyapihan Ventilasi Mekanik Di Intensive Care Unit: Studi Literatur Sarinti, Sarinti; Utami, Reni Sulung; Prasetyo, Awal
Jurnal Smart Keperawatan Vol 3, No 1 (2016): Juni 2016
Publisher : Sekolah Tinggi Ilmu Kesehatan (STIKes) Karya Husada Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34310/jskp.v3i1.463

Abstract

Latar belakang : Pasien di ICU ( Intensive Care Unit ) yang membutuhkan bantuan ventilasi mekanik hampir mencapai 30% dari total pasien. Ventilasi mekanik yang digunakan dalam jangka panjang dapat meningkatkan resiko terjadinya mortalitas, dan ventilator associated pneumonia sehingga diperlukan perencanaan penyapihan yang tepat.  Tujuan : Studi literatur ini bertujuan untuk mengidentifikasi metode penyapihan pasien dari ventilasi mekanik di ICU. Metode : Penelusuran literatur dilakukan melalui database Google scholar dan Google search dengan menggunakan kata kunci weaning mechanical ventilation, weaning protocol, critical care nursing. Literatur yang digunakan dari tahun 1997-2015 dalam bentuk full text. Literatur yang sesuai kriteria inklusi dan eksklusi dianalisis secara narasi. Hasil: Penelusuran mendapatkan 7 artikel penelitian yang menunjukkan metode penyapihan pasien dari ventilasi mekanik. Metode penyapihan dari ke 7 artikel tersebut adalah metode protokol dan non protokol. Metode non protokol misalnya physician direct weaning ( PDW ) dan collaboration weaning plan ( CWP ). Metode penyapihan dengan protokol antara lain : nurse` protokol directed weaning ( NPDW ) dan protokol base weaning ( PBW ). Kesimpulan : Metode penyapihan protokol menunjukkan hasil lebih efektif dibandingkan metode non protokol. Kata Kunci: Penyapihan Ventilasi Mekanik, Protokol Penyapihan, Keperawatan Kritis Methode of Weaning from Mechanical Ventilation in Intensive Care Unit : Study Literature ABSTRACT Back ground : patients in the ICU who require mechanical ventilation assistance almost 30% of the total patients. Mechanical ventilation is used in the long term can increase the risk of mortality and ventilator associated pneumonia so that proper planning is necessary weaning. Objectives : the literature study aims to identify methods of weaning patients from mechanical ventilation in the ICU. Methods : the literature study search pass through by Google scholar and Google search using keywords weaning mechanical ventilation, weaning protocol, critical care nursing. Literature is used from the year 1997-2015 in the form of full text. Appropriate literature inclusion and exclusion criteria were analyzed narrative. Results : search get 7 articles studies showing methods of weaning patients from mechanical ventilation. Method of weaning to the article 7 is the method protocol and non-protocol. Methods of non protocols eg physician-direct weaning ( PDW ) and collaboration weaning plan ( CWP ). Methods of weaning protocols include: nurse` protocol directed weaning (NPDW) and protocols base weaning ( PBW ). Conclusion : the method of weaning protocol shows the results more effective than non - protocol methods Key word : weaning mechanical ventilation; weaning protocol; critical care nursing
Granzyme-B Expression as a Predictor of Immune Surveillance (Correlation Study in Patient with Squamous Cell Carcinoma and Uterine Cervical Dysplasia) Apriana, Ledisda; Udadi Sadhana; Ika Pawitra Miranti; Awal Prasetyo; Dik Puspasari
Biomedical Journal of Indonesia Vol. 7 No. 3 (2021): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v7i3.585

Abstract

Background: The most common histologic type of cervical cancer is squamous cell carcinoma (SCC), with the risk of recurrence depending on immune surveillance with granzyme-B markers, and tumor infiltrating lymphocytes (TIL) is an immunological determinant associated with a better prognosis. Granzyme-B is produced by TIL. The study was aimed to prove the difference in expression of granzyme-B in SCC epithelial cells with cervical dysplasia precancerous lesions as a predictor of immune surveillance. Methods: Correlation analytic study with cross-sectional design on paraffin block patients with SCC and cervical epithelial dysplasia at the Anatomical Pathology Laboratory, RSUP Dr. Kariadi, Semarang, during 2018 and 2019, using granzyme-B immunohistochemistry assays, assessed TIL activation. Results: Most cases of cervical SCC were 40 years old, 50% were stage IIIB, 20% with the final condition died on observation 1 year after the initial diagnosis was made. The uterine cervical SCC group expressed granzyme-B with a mean score of 5.81±1.17 (p=0.001) and the dysplastic epithelial lesion group with a mean score of 6.83±0.95 (p=022), the Mann-Whitney test showed p value = 0.009, so that the test results are statistically significant. Conclusion: Expression of granzyme-B in dysplasia is higher than in uterine cervical SCC.
Particular Matter of Motor Vehicle Exposure and High-Fat Diet Effects on Kidney Histopathology, Creatinine, and Malondialdehyde (MDA) Levels in Wistar Rats Veni, Rizka; Prasetyo, Awal; Muniroh, Muflihatul
Indonesian Journal of Environmental Management and Sustainability Vol. 5 No. 3 (2021): September
Publisher : Research Centre of Inorganic Materials and Complexs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26554/ijems.2021.5.3.124-128

Abstract

This study aims to analyze the effect of combination of motor vehicle particular matter exposure and high-fat diet in kidney histopathology, creatinine levels, and MDA levels in Wistar rats. This study used a posttest-only control group design. Eighteen healthy male Wistar rats were divided into three groups. The intervention groups received motor vehicle fume exposure for 100 s with normal diet (X1) or high-fat diet (X2), and the control group received no exposure (C). Data analysis was processed with a SPSS 25.0 computer program by using the one-way ANOVA test followed by post hoc LSD. The degree of kidney histopathological damage showed significant differences between the X1 and X2 groups when compared with the control group (p < 0.05). The results of the creatinine level examination found a significant difference between the X2 and C groups (p < 0.05) and the treatment groups X1 and X2 (p < 0.05). The results of kidney MDA level examination showed a significant difference between the treatment groups (X1 and X2) and the control group (p < 0.05). The combination of particular matter of motor vehicle fumes exposure and high-fat diet could induce kidney damage through histopathological change and increased creatinine levels and kidney MDA levels in Wistar rats.
Perbedaan Kadar IgA dan IL6 Pada Penumpang “Biosmart and Safety Bus” pada Memakai Masker Herbal dan Masker Medis Rahajeng, Herera; Prasetyo, Awal; Susilaningsih, Neni; Sadhana, Udadi; Maharani, Nani; Asri, Hairul
Life Science Vol 10 No 1 (2021): April 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v10i1.47177

Abstract

Viral infections that attack the respiratory tract are currently a major problem globally. The spread of viral infection can be one of the risks by passengers of the bus. The government's efforts being developed are the application of biosmart and safety buses designed to prevent the virus with stake holders. One of the health protocols is the use of masks. The purpose of this study was to see the differences in IgA and IL6 levels before and after the use of medical masks and herbal masks. Nasal wash samples were taken and analyzed by ELISA. The results obtained were the level of IgA for the control group before wearing medical masks was 23.46834 pg/ml and after wearing 29.74473 pg/ml. While the IL6 levels before were 1.10544 ng / ml and after -2.04119 ng/ml. The results of the IgA in the experimental group before using the herbal mask 24.00840 pg/ml and after using was 29.74473 pg/ml. Meanwhile, the level of IL6 before was 0.91682 ng/ml and after 0.500157 ng/ml. Based on the results, there was an increase in IgA levels after the use of medical masks and herbal masks while IL6 levels decreased after the use of medical and herbal masks. Keywords: Herbal mask; IgA; IL6, Masker herbal;IgA;IL6 Infeksi virus yang menyerang saluran pernapasan saat ini menjadi masalah utama secara global. Penyebaran agen infeksi virus dapat menjadi salah satu risiko yang dihadapi penumpang moda transportasi Bus. Upaya Pemerintah seperti yang saat ini dikembangkan adalah pengaplikasian biosmart and safety bus yang dirancang guna menghambat persebaran virus dengan stake holder yang tepat. Salah satu penerapan protokol kesehatan adalah penggunaan masker. Tujuan dari penelitian ini adalah melihat perbedaan kadar IgA dan IL6 sebelum dan sesudah pemakaian masker medis dan masker herbal pada penumpang biosmart and safety bus. Pengambilan sampel nasal wash dilakukan dan dianalisis dengan ELISA. Penelitian ini merupakan quasi experimental dengan prepost test randomized control design. Hasil yang didapat adalah kadar IgA untuk kelompok kontrol sebelum pemakaian masker medis 23,46834 pg/ml dan setelah memakai masker medis 29,74473 pg/ml. Sementara kadar IL6 sebelum adalah 1,10544 ng/ml dan setelah -2,04119 ng/ml. Hasil IgA kelompok eksperimen sebelum pemakaian masker herbal 24,00840 pg/ml dan setelah memakai masker herbal 29,74473 pg/ml. Sementara kadar IL6 sebelum adalah 0,91682 ng/ml dan setelah 0,500157 ng/ml. Berdasarkan hasil penelitian terdapat peningkatan kadar IgA setelah pemakaian masker medis dan masker herbal sementara kadar IL6 mengalami penurunan setelah pemakaian masker medis dan masker herbal. Keywords: Herbal mask; IgA; IL6, Masker herbal;IgA;IL6
Ekspresi EBER pada Berbagai Tipe Histopatologi Karsinoma Nasofaring Kenty Wantri Anita; Bambang Endro Putranto; Awal Prasetyo; Dewi Kartikawati Paramita
Majalah Patologi Indonesia Vol 24 No 1 (2015): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.249 KB)

Abstract

ABSTRAK Latar belakang Etiologi kanker nasofaring (KNF) sudah terbukti merupakan interaksi dari infeksi Epstein-Barr Virus (EBV), paparan lingkungan dan kerentanan genetik. Infeksi EBV mendominasi etiopatogenesis KNF terutama untuk tipe WHO-3. Di Indonesia, saat ini belum ada publikasi tentang ekspresi Epstein-Barr-virus-encoded small nuclear RNA (EBER) yang dikaitkan dengan klasifikasi histopatologi KNF. Tujuan penelitian ini menganalisis ekspresi EBER pada berbagai tipe histopatologi KNF untuk menambah informasi dalam diagnosis etiologi KNF, sehingga bermanfaat dalam strategi terapi. Metode Penelitian dengan desain cross sectional ini menggunakan sampel biopsi nasofaring yang didiagnosis sebagai KNF antara tahun 2009 sampai 2012 di Laboratorium Patologi Anatomik Fakultas Kedokteran Universitas Diponegoro/RSUP. Dr. Kariadi dan RS. St. Elisabeth Semarang. Sediaan dievaluasi ulang dan dilakukan in situ hybridization dengan probe fluorescein-conjugated peptide nucleic acid (PNA) pada jaringan blok parafin. Hasil Sebanyak 29 dari 31 kasus (93,5%) KNF tipe WHO-3 mengeskpresikan EBER positif dan terdapat perbedaaan bermakna (p=0,024) dengan KNF tipe WHO-1 dan 2. Uji korelasi antara ekspresi EBER dengan tipe histopatologi KNF positif kuat (koefisien korelasi Lambda = 0,667, p=0,034). Kesimpulan Ekspresi EBER terdapat lebih banyak pada KNF WHO tipe 3 yang berarti EBV lebih sering ditemukan pada KNF WHO tipe 3. Kata kunci: EBER, hibridisasi in situ, karsinoma nasofaring, virus Epstein-Barr. ABSTRACT Background The factors contributing nasopharingeal carcinoma (NPC) has been proven to be the interaction of viral, environmental and genetic factors. Etiopathogenesis of NPC is dominated by Epstein-Barr virus (EBV) infection, especially for WHO type 3. In Indonesia, there has been no publication about the expression of Epstein-Barr-virus-encoded small nuclear RNA (EBER) associated with histopathological classification. The aim of this study is to analyze the expression of EBER in various histopathological types of NPC and provide information onetiological diagnosis ofNPC that can be useful for therapeutic strategies. Methods This study was an descriptive designed as cross sectional study. Nasopharyngeal biopsies that were diagnosed as nasopharyngeal carcinoma, between the year 2009 and 2012, in Dr. Kariadi Hospital and St. Elisabeth Hospital Semarang were reevaluated. In situ hybridization with fluorescein-conjugated peptide nucleic acid (PNA) probe was performed on paraffin embedded tissue sections. Results This study found that 29 of 31 (93.5%) WHO-3 type NPC cases were EBER positif that significantly differed from WHO-1 and WHO-2 type NPC (p=0.024). There were strong positive correlation between histopathological types of NPC and expression of EBER (Lambda coefficient correlation=0.667, p=0.034). Conclusion EBER expressions found more in NPC WHO type 3 which mean EBV is often found in NPC WHO type 3. Key words: EBER, Epstein-Barr- virus, in situ hybridization, nasopharyngeal carcinoma.
Co-Authors - Anggorowati Adhi Setradian Anto Maria Aditya Purnama Agus Subagio Ahmad Hamim Sadewa Albertus Ari Adrianto Alendra Chakramurty Alfin Ihza Trimahendra Amira Naufal Andreas Arie Setiawan, Andreas Arie Anisa Ell Raharyani Anna Jumatul Laely Antari, Arlita Leniseptaria Apriana, Ledisda Ariani, Resti Arthur Lelono Arum Kartikadewi Asri, Hairul Astika Widy Utomo Astuti, Meira Dewi Kusuma Ayu Aksara Bagus Ropyanto, Chandra Bambang Endro Putranto Bambang Hariwiyanto Cahyono Hadi Cahyono Hadi Chandra Bagus Ropyanto Dadi Hamdani Darryl Samuel Salim Devia Eka Listiana Devia Eka Listiana, Devia Eka Dewi Kartikawati Paramita Dik Puspasari Dik Puspasari Dik Puspasari, Dik Djaswadi Dasuki Djaswadi Dasuki Em Sutrisna Eriawan Agung Nugroho Gufron, Ahmad Halim, Fenny Hendro Prabowo Hermawan Istiadi Hermawan Istiadi Heru Suwardianto Hidayat Sulistyo Hidayat Sulistyo Ika Pawitra Miranti Ika Pawitra Miranti Indah Saraswati Indwiani Astuti Indwiani Astuti Jeffrey Eka Wijaya Jessica Christanti Jumatul Laely, Anna K. Heri Nugroho HS, K. Heri Karlowee, Vega Kenty Wantri Anita, Kenty Wantri Khasan Rowi Kurniawan, Eirin Yovita Kusmiyati Tjahjono Kusmiyati Tjahjono Lisyani Budipradigdo Meira Dewi Kusuma Astuti Mentari, Alfitra Akbar Bara Mentari Mochamad Ali Sobirin Mohammad Hakimi Muniroh, Muflihatul Muzakar Isa Nani Maharani Neni Susilaningsih Nur, Rizki Widya Nurhayadi, Tommy Nurjaya Adinugroho Nurkholis, Fathur Ockta, Yovhandra Puspita`, Rina Putra, Dominikus Evano Rahajeng, Herera Redyaksa Drestanta Ariandoko Reni Sulung Utami Sarinti Sarinti Sarinti, Sarinti Selvia David Richard Siahaan, Johanna Soenarto Sastrowijoto Sony Noegroho Sri Jumini Stefani Candra Firmanti Su, Hansen Maikel Sugiri, Sugiri Suhartini Ismail, Suhartini Taufiq R Nasihun Taufiq, Heydar Ruffa Tjahjono Tjahjono Tjahjono Tri Nur Kristina Udadi Sadhana Udadi Sadhana, Udadi Vega Karlowee Veni, Rizka Wahyu Haris Prabowo Wahyuningsih, Indah S Wiratno Wiratno Yuriz Bakhtiar Yusuf, Franky