Low Class Ib (HLA-G/Qa-2) MHC Protein Expression against Hsp-70 and VCAM-1 Profile on Preeclampsia. An observation on experimental animal Mus Musculus with Endothelial Dysfunction model

Sulistyowati, Sri

Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia)
Volume. 34, No. 3, July 2010

Objective: To analyze the expression of MHC class Ib Qa-2 protein
(homologue to HLA-G in human), Hsp-70, and VCAM-1 in trophoblast
cells of preeclampsia and control using endothelial dysfunction
model on Mus Musculus.

Method: Design of study is experimental study. Pregnant mice
was treated with anti-Qa-2. Hsp-70 and VCAM-1 expressions of
trophoblast cells was assessed on week I, II, and III.

Result: Negative Qa-2 expression was achieved after administration
of 40ng anti Qa-2 on the fifth day of pregnancy. The Hsp-70
and VCAM-1 expressions in negative Qa-2 mice (preeclampsia)
was higher compared with those of positive Qa-2 mice (normal
pregnancy) on week I, II and III.

Conclusion: Low Qa-2 and high of HSP-70 and VCAM-1 expression
might be useful for prediction of preeclampsia or endothelial
dysfunction.

[Indones J Obstet Gynecol 2010; 34-3: 103-7]

Keywords: preeclampsia, HLA-G/Qa-2, Hsp-70, VCAM-1, endothelial
dysfunction