<![CDATA[Objective: To evaluate the expression of PPARy receptor and to
compare its expression in eutopic and ectopic endometrium in
women with endometriosis.
Method: This is a cross sectional study. Ten female subjects with endometriosis
that underwent laparoscopy or laparotomy who fulfilled
the inclusion criteria were recruited by consecutive sampling.
Two samples were taken, eutopic endometrium and ectopic endometrium
from endometriosis cyst wall during surgery of each
subject. PPARy expression was examined by two-step RT-qPCR. Our
data was statistically examined using the paired t-test and Pearson’s
correlation test.
Result: PPARy was found to be expressed in eutopic and ectopic endometrium
of women with endometriosis using the RT-qPCR method.
The expression of PPARy was not statistically different in
eutopic and ectopic endometrium (1.16 relative fold vs 1.25 relative
fold; p=0.26). By Pearson’s correlation there was a weak positive
correlation between PPARy expression of eutopic and ectopic endometrium
(r=0.16).
Conclusion: PPARy was detected by two-step RT-qPCR in eutopic
and ectopic endometrium of women with endometriosis. Semiquantification
of PPARy expression showed that there was no significant
difference between PPARy expression in eutopic and ectopic endometrium
of women with endometriosis. There was a weak positive
correlation between PPARy expression in eutopic and ectopic endometrium
of women with endometriosis.
[Indones J Obstet Gynecol 2015; 3-4: 200-205]
Keywords: endometriosis, PPARy, two-step RT-qPCR]]>
Copyrights © 2015
