<![CDATA[Objective: To prove the effect of ethinyl estradiol as an activator on
human Telomerase Reverse Transcriptase (hTERT).
Method: The experimental study was conducted in vitro by using
culture of complete hydatidiform mole trophoblast cell. We exposed
the culture to ethinyl estradiol in varied doses and measured the
concentration of hTERT through RT-PCR quantitative. There were
40 specimens as control group and 20 specimens exposed to ethinyl
estradiol in different doses (10, 20, 40 and 80 mcg) as experimental
group. The activity of hTERT was measured by RT-PCR and the
concentration of it was assessed by ELISA. We analyzed the variables
using ANOVA, Turkey post hoc and Pearson correlation test.
Result: In control group, the concentration of hTERT was not detected.
Meanwhile, the concentration among different doses of
ethynil estradiol (10, 20, 40, 80 mcg) was 113,117.5; 114,507.6;
102,193.9; 127.546.1 amoles/ml, respectively. Among experimental
group, they were significantly different both using F test (ANOVA)
(p=0.001) and Turkey post hoc test (p=0.005). The correlation
among group was 0.84 which meant higher level of ethinyl estradiol
was correlated with higher activity of hTERT.
Conclusion: Ethinyl estradiol impacts to the increase of hTERT activity
on complete hydatidiform mole cell culture.
[Indones J Obstet Gynecol 2016; 4-2: 93-96]
Keywords: complete hydatidiform mole, ethinyl estradiol, human
Telomerase Reverse Transcriptase (hTERT)]]>
Copyrights © 2016
