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HUBUNGAN INDEKS MASSA TUBUH (IMT) DENGAN RESPON KLINIS KEMORADIASI PASIEN KANKER SERVIKS STADIUM III DI RSUD Dr. SAIFUL ANWAR MALANG Werestandina, Adys; Nurseta, Tatit; Nugroho, Fajar Ari
Majalah Kesehatan FKUB Vol 4, No 1 (2017): MAJALAH KESEHATAN FAKULTAS KEDOKTERAN
Publisher : Faculty of Medicine Universitas Brawijaya

Kanker serviks merupakan gangguan yang terjadi pada sel somatik, ketika perubahan materi genetik menyebabkan sel normal berperilaku abnormal. Pada kanker serviks stadium III dilakukan pengobatan standar yaitu kemoradiasi. Respons klinis kemoradiasi dipengaruhi beberapa faktor salah satunya yaitu indeks massa tubuh (IMT). Penelitian ini bertujuan mengetahui hubungan indeks massa tubuh dengan respons klinis kemoradiasi pasien kanker serviks stadium III. Desain penelitian yaitu observasi dengan pendekatan cohort retrospective menggunakan data rekam medis pasien kanker serviks di RSUD dr. Saiful Anwar Malang. Data rekam medis yang digunakan sebanyak 27 pasien. Analisis data dengan uji korelasi Spearmanâ€™s rho, didapatkan hubungan yang signifikan antara indeks massa tubuh terhadap respons klinis kemoradiasi dengan nilai P = 0,001 (0.001 < Î±=0.05). Uji hubungan keeratan dengan correlation coefficient didapatkan hasil -0,594, yang berarti bahwa ada hubungan yang kuat namun berkebalikan antara IMT dengan respons klinis.Â Kata kunci: IMT, kanker serviks, kemoradiasi.

The effect of 17Î² Estradiol Exposure on Mutant p53 Expression in Hydatidiform Mole Trophoblast Cell Culture Nurseta, Tatit
Indonesian Journal of Obstetrics and Gynecology Volume. 35, No. 1, January 2011
Publisher : Indonesian Socety of Obstetrics and Gynecology

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Objectives: To compare the mutant p53 expression in normal trophoblast (N) cell culture with hydatidiform mole trophoblast (HM) cell culture which was exposed to 17Î² estradiol. Methods: An experimental study conducted at the Laboratory of Physiology Faculty of Medicine, Brawijaya University Malang using N cell culture and HM cell culture with 17Î² estradiol exposure. Trophoblast cell culture of normal and hydatidiform mole was divided in 6 groups, such as: 1. Without added 17Î² estradiol; 2. Added 5 nm 17Î² estradiol; 3. Added 10 nm 17Î² estradiol; 4. Added 20 nm 17Î² estradiol; 5. Added 40 nm 17Î² estradiol; 6. Added 80 nm 17Î² estradiol. Then performed immunocytochemistry staining using p53 mutant primary antibody and observed the expression of p53 mutant. Data from observations analized with the ANOVA test and correlation test. Results: Mutant p53 expression in N cell culture showed no significant differences in each treatment dose of 17Î² estradiol (p = 0086 > 0.05). The dose at 80nm 17Î² estradiol showed an average of highest mutant p53 expression on N cell culture rather than giving the dose of 17Î² estradiol on 40 nm, 20 nm, 10nm and 5 nm. While the control group showed a lowest average of mutant p53 expression in N cell culture when compared to the treatment group which was exposed to 17Î² estradiol. Mutant p53 expression in HM cell culture showed a significant difference at each treatment dose of 17Î² estradiol (p = 0.000 < 0.05). The existence of the effect of 17Î² estradiol begins when the expression of mutant p53 in HM cell culture becomes higher after being given treatment in the form of 17Î² estradiol on the dose of 5 nm compared with the expression of 17Î² estradiol in the control group. Then the expression of mutant p53 in HM cell culture is increasing when given doses of 17Î² estradiol at 20 nm and 40 nm. At a dose of 40 nm it shows the highest expression of mutant p53. Expression of mutant p53 in HM cell culture decreased when given at doses 80 nm. Conclusion: Mutant p53 expression in N cell culture exposed to 17Î² estradiol showed no significant difference. Expression of mutant p53 in HM cell culture which was exposed to 17Î² estradiol showed a significant difference. Mutant p53 expression in N and HM cell culture which was exposed to 17Î² estradiol showed significantly different, in which mutant p53 expression in N cell culture is lower than the expression of mutant p53 in HM tissue culture. [Indones J Obstet Gynecol 2011; 35-1: 30-5] Keyword: p53 mutant, 17Î²-estradiol, hydatidiform mole

The Effect of Extra Virgin Olive Oil (EVOO) on Expression of Vascular Endhothelial Growth Factor (VEGF) and Arteriole Number on Endometrium of Female Rattus norvegicus of Wistar Strain Exposed to Rhodamin B Anisak, Siti; Sujuti, Hidayat; Sutrisno, Sutrisno; Mintaroem, Karyono; Nurseta, Tatit; Kalsum, Umi
Health Notions Vol 2 No 8 (2018): August 2018
Publisher : Humanistic Network for Science and Technology (Address: Cemara street 25, Ds/Kec Sukorejo, Ponorogo, East Java, Indonesia 63453)

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Impact of Ethinyl Estradiol to Human Telomerase Reverse Transcriptase Activity on Complete Hydatidiform Mole Culture Nurseta, Tatit; Wijasa, Arsana; Sitompul, Barlian
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To prove the effect of ethinyl estradiol as an activator on human Telomerase Reverse Transcriptase (hTERT). Method: The experimental study was conducted in vitro by using culture of complete hydatidiform mole trophoblast cell. We exposed the culture to ethinyl estradiol in varied doses and measured the concentration of hTERT through RT-PCR quantitative. There were 40 specimens as control group and 20 specimens exposed to ethinyl estradiol in different doses (10, 20, 40 and 80 mcg) as experimental group. The activity of hTERT was measured by RT-PCR and the concentration of it was assessed by ELISA. We analyzed the variables using ANOVA, Turkey post hoc and Pearson correlation test. Result: In control group, the concentration of hTERT was not detected. Meanwhile, the concentration among different doses of ethynil estradiol (10, 20, 40, 80 mcg) was 113,117.5; 114,507.6; 102,193.9; 127.546.1 amoles/ml, respectively. Among experimental group, they were significantly different both using F test (ANOVA) (p=0.001) and Turkey post hoc test (p=0.005). The correlation among group was 0.84 which meant higher level of ethinyl estradiol was correlated with higher activity of hTERT. Conclusion: Ethinyl estradiol impacts to the increase of hTERT activity on complete hydatidiform mole cell culture. [Indones J Obstet Gynecol 2016; 4-2: 93-96] Keywords: complete hydatidiform mole, ethinyl estradiol, human Telomerase Reverse Transcriptase (hTERT)

Curcumin Administration on Proliferation and Apoptosis Index in Complete Hydatidiform Mole CellCulture Nurseta, Tatit; Irwanto, Yahya; Imelda, Imelda
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 1, January 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To investigate that curcumin can decreasing proliferation index and increasing apoptosis index. Method: This is an experimental non-blinded study with post test control group design, at Cell/Tissue Culture Laboratory at Medical Faculty of Brawijaya University. This study using CHM trophoblastic cell culture from CHM curettage patient, exposed by several doses of curcumin, 0, 50, 100, 200, 400 and 800 μM, then examined by the method of MTT proliferation index and apoptosis index by the method of labeling DNA fragmentation TUNEL system. Data analyzed by one-way ANOVA. Result: In this study, the mean values obtained decrease in proliferation index with increasing doses of curcumin. Giving a dose of 200 μM curcumin, 400 μM and 800 μM proved highly significant (p = 0.001) reduced proliferation index compared with the control and curcumin dose of 50 μM and 100 μM. From this research shows that there are significant differences in the increase of apoptosis index (p = 0.001) between the control group with curcumin dose group 200 μM, 400 μM and 800 μM. But there was no significant difference in the mean of apoptosis index among the three dose groups. Conclusion: Giving curcumin dose of 200 μM can decreasing the proliferation index and increasing the apoptosis index increases in CHM trophoblastic cell culture. [Indones J Obstet Gynecol 2016; 1: 37-41] Keywords: apoptosis index, curcumin, proliferation index

The Effects of Glucomannan Hydrolysates and BV Gel on Nugent Score, Treg Cell Percentage, and TGF-? level in Bacterial Vaginosis Retnoningrum, Ambar Dwi; Nurseta, Tatit; Prawiro, Sumarno Reto; Endharti, Agustina Tri; Wahyuni, Endang Sri
Indonesian Journal of Medicine Vol 3, No 1 (2018)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

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Background: Bacterial vaginosis is commonly experienced by women of reproductive age. The Nugent score is the gold standard for diagnosing bacterial vaginosis. Prebiotic Glucomannan hydrolysates (GMH) as a therapy in the treatment of bacterial vaginosis and has an immunomodulatory effect on the immune system and provides cellular immunity. The purpose of this study was to examine the effects of GMH and BV Gel on Nugent scores, Treg cell presentation, and TGF ? levels in bacterial vaginosis of women of childbearing age.Subjects and Method: This was an experimental study. A sample of 24 women aged 20 to 45 years old with bacterial vaginosis (Nugent score ?7) was divided into 4 groups: oral antibiotic group metronidazole (500mg), combination of GMH (300mg) and metronidazole, 5 ml BV Gel tube, and combination of GMH and BV Gel scores. The dependent variables were GMH and BV Gel administrations. The independent variables Nugent score, Treg presentation, and TGF-? level. Nugent score, Treg cell presentation, and TGF ? level were measured on day-0, day-11, and day-22. The data were analyzed by one way Anova.Results: The results of the analysis after treatment on day 22 showed that the GMH and BV gel were able to reduce Nugent scores, increase Treg cell presentation and TGF ? levels in bacterial vaginosis of women of childbearing age.Conclusion: GMH as an alternative therapy for bacterial vaginosis compared with antibiotic treatment can improve normal vaginal flora and stimulate the immune system in vitro and in vivo significantly.Keywords: Glucomannan Hydrolysates, BV Gel, Nugent sel Treg score, TGF ?Correspondence:Ambar Dwi Retnoningrum. Masters Program of Midwifery, Universitas Brawijaya, Malang, East Java. Email: adreambar@gmail.com. Mobile: +6281335743696.Indonesian Journal of Medicine (2018), 3(1): 33-43https://doi.org/10.26911/theijmed.2018.03.01.05

Curcumin Administration on Proliferation and Apoptosis Index in Complete Hydatidiform Mole CellCulture Nurseta, Tatit; Irwanto, Yahya; Imelda, Imelda
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 1, January 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To investigate that curcumin can decreasing proliferation index and increasing apoptosis index. Method: This is an experimental non-blinded study with post test control group design, at Cell/Tissue Culture Laboratory at Medical Faculty of Brawijaya University. This study using CHM trophoblastic cell culture from CHM curettage patient, exposed by several doses of curcumin, 0, 50, 100, 200, 400 and 800 μM, then examined by the method of MTT proliferation index and apoptosis index by the method of labeling DNA fragmentation TUNEL system. Data analyzed by one-way ANOVA. Result: In this study, the mean values obtained decrease in proliferation index with increasing doses of curcumin. Giving a dose of 200 μM curcumin, 400 μM and 800 μM proved highly significant (p = 0.001) reduced proliferation index compared with the control and curcumin dose of 50 μM and 100 μM. From this research shows that there are significant differences in the increase of apoptosis index (p = 0.001) between the control group with curcumin dose group 200 μM, 400 μM and 800 μM. But there was no significant difference in the mean of apoptosis index among the three dose groups. Conclusion: Giving curcumin dose of 200 μM can decreasing the proliferation index and increasing the apoptosis index increases in CHM trophoblastic cell culture. [Indones J Obstet Gynecol 2016; 1: 37-41] Keywords: apoptosis index, curcumin, proliferation index

Impact of Ethinyl Estradiol to Human Telomerase Reverse Transcriptase Activity on Complete Hydatidiform Mole Culture Nurseta, Tatit; Wijasa, Arsana; Sitompul, Barlian
Indonesian Journal of Obstetrics and Gynecology Volume. 4, No. 2, April 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To prove the effect of ethinyl estradiol as an activator on human Telomerase Reverse Transcriptase (hTERT). Method: The experimental study was conducted in vitro by using culture of complete hydatidiform mole trophoblast cell. We exposed the culture to ethinyl estradiol in varied doses and measured the concentration of hTERT through RT-PCR quantitative. There were 40 specimens as control group and 20 specimens exposed to ethinyl estradiol in different doses (10, 20, 40 and 80 mcg) as experimental group. The activity of hTERT was measured by RT-PCR and the concentration of it was assessed by ELISA. We analyzed the variables using ANOVA, Turkey post hoc and Pearson correlation test. Result: In control group, the concentration of hTERT was not detected. Meanwhile, the concentration among different doses of ethynil estradiol (10, 20, 40, 80 mcg) was 113,117.5; 114,507.6; 102,193.9; 127.546.1 amoles/ml, respectively. Among experimental group, they were significantly different both using F test (ANOVA) (p=0.001) and Turkey post hoc test (p=0.005). The correlation among group was 0.84 which meant higher level of ethinyl estradiol was correlated with higher activity of hTERT. Conclusion: Ethinyl estradiol impacts to the increase of hTERT activity on complete hydatidiform mole cell culture. [Indones J Obstet Gynecol 2016; 4-2: 93-96] Keywords: complete hydatidiform mole, ethinyl estradiol, human Telomerase Reverse Transcriptase (hTERT)

The Effect of Human Pellucide Zone 3 Monoclonal Antibody on Expression of Bcl-2 and Bax in Follicle Granulosa Cells of Mice Ovary Natalina, Riny; Nurseta, Tatit; Winarsih, Sri
Journal of Tropical Life Science Vol 8, No 2 (2018)
Publisher : Journal of Tropical Life Science

Pellucide zone 3 (ZP3) involves in fertilization mechanism. Moreover, antibody of ZP3 can develop to inhibit egg and sperm interaction. This study aims to determine the effect of hZP3 (mab-hZP3) monoclonal antibody on the expression of Bcl-2 and Bax in follicle granulosa cells of the mice ovary. Female mice BALB/c were divided into 12 groups which consisted of control and experimental treatment group. Each group was added with 30% of total mice as error sample (1 mice). Each groups were treated differently: 50 Âµl adjuvant Al(OH)3 in 50 Âµl Tris HCl, 20 Âµg Mab-hZP3, 40 Âµg Mab- hZP3, and 60 Âµg Mab-hZP3. Each group was dissected at day 10, 15 and 20. Measurement of Bcl-2 and Bax was performed with immunohistochemistry. Data was analyzed by Two-Way ANOVA. There was no significant effect of Mab-hZP3 administration in various doses on Bcl-2 (p=0.0825), and Bax (p=0.836). There was no significant effect of administration of Mab-hZP3 in time (p=0.807), neither on Bcl-2 expression (p=0.088) and Bax (p=0.227). The lowest Bcl-2 level was found in dose of 60 Âµg in day 15. There was no significant effect of Mab-hZP3 in various doses and time (p=0.691), neither to Bcl-2 and Bax. Such results obtained due to the specificity of a monoclonal antibody that recognizes specific antigen. Mab-hZP3 is proposed as immunocontraception for women causing no disturbance of folliculogenesis.

Agarose Coated Culture Plate in Tumorsphere Culture of Cervical Cancer Cell Line HeLa: an Alternative to Non Adhesive Culture Plate Juniartha, Putu; Indra, Muhammad Rasjad; Sujuti, Hidayat; Lyrawati, Diana; Nurseta, Tatit
Journal of Tropical Life Science Vol 6, No 3 (2016)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.06.03.11

Cervical cancer recurs in 90% cases and linked to cancer stem cells that able to self-renew and responsible for recurrence, metastasis, and mortality of cancer. Isolation and identification of cancer stem cells using serum-free medium needs expensive growth factors and consume time. This study try to grow tumor sphere using culture plate coated with 1% agarose as an efficient and economical alternative to non-adhesive culture plate. HeLa cell line was grew in culture plate coated with 1% agarose and non-adhesive culture plate using similar medium and culture condition. Tumor spheres morphology was observed and the colonies were counted in 7 days followed by single cell assay. Tumor spheres then counted for CD133+, CD34+, and Sox2 expression using flowcytometry. Culture plate coated with 1% agarose can be used as an economic and efficient alternative to culture tumor sphere. Using culture plate coated with 1% agarose, the tumor spheres formed in 7 days with similar morphology to non-adhesive culture plate. Tumorsphere had three dimensional â€“ sphere shape that tightly attached, colonized, and overlapped. The tumor sphere colony counts of two plates were statistically have no significant difference (p=0,667). Single cell assay of a tumor sphere shows that it can grow new tumor spheres with similar morphology. The tumor sphere from culture plate coated with 1% agarose express CD133+ and CD34+ as much as 8.78% Â± 2.14 and Sox2 as much as 35.30% Â± 23.82 whereas tumor sphere from non-adhesive culture plate express CD133+ and CD34+ as much as 62.36% Â± 1.06 and Sox2 as much as 98.86% Â± 0.56 (p = 0000).

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