Efek Pentagamavunon-0 terhadap Konsentrasi cAMP dan Progesteron pada Kultur Sel Luteal yang mengandung Teofilin

Purwaningsih, Endang ( Department of Anatomy (Biology), Faculty of Madicine, YARSI University, Jakarta)
Kadarsih Soejono, Sri ( Department of Physiology, Faculty of Medicine, Gadjah Mada University, Yogyakarta)
Dasuki, Djaswadi ( Department of Obstetric and Gynecology, Faculty of Madicine, Gadjah Mada University, Yogyakarta)
Meiyanto, Edy ( Department of Molecular Biology, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta)

YARSI Medical Journal
Vol 17, No 2 (2009): MEI - AGUSTUS 2009

Curcumin analog (Pentagamavunon-0/PGV-0) can inhibit steroidogenesis of luteal cell culture. Corpus luteum secretes progesterone by LH stimulation. The main transduction signal of luteal cells steroidogenesis is through the cAMP/PKA. The objective of this study was to know the effect of PGV-0 on cAMP and progesterone concentration of luteal cell culture containing theophylline. The subject was corpus luteum of rat Sprague Dawley strain induced with PMSG (10 IU). PGV-0 was given shortly after the stimulation of LH and or PGF2? with or without theophyline. The cell culture then put into the incubator for 24 hours. Concentration of cAMP was assessed by ELISA whereas the progesterone concentration was determined by RIA. The result showed that LH stimulation caused cAMP and progesterone increase significantly. The inhibition of PGF2? on cAMP and progesterone concentrations showed no significant difference compared to the control. Theophylline increased the cAMP and progesterone concentration significantly but not to LH stimulation. PGV-0 did not inhibit cAMP concentration but PGV-0 inhibited the progesterone concentration by LH stimulation. In conclusion, PGV-0 inhibits signal transduction of lutheal cell in down stream cAMP.