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Kadarsih Soejono, Sri
Department of Physiology, Faculty of Medicine, Gadjah Mada University, Yogyakarta
Medicine & Pharmacology

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Pengaruh Kurkumin pada Kultur Sel Luteal Tikus yang mengandung Teofilin terhadap Kadar cAMP dan Progesteron Purwaningsih, Endang; Kadarsih Soejono, Sri; Dasuki, Djaswadi; Meiyanto, Edy
Jurnal Kedokteran YARSI Vol 17, No 3 (2009): SEPTEMBER - DESEMBER 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (286.417 KB) | DOI: 10.33476/jky.v17i3.210

Abstract

Senyawa kurkumin dapat menghambat steroidogenesis kultur sel luteal dengan menghambat sekresi progesteron. Letak kerja kurkumin pada steroidogenesis kultur sel luteal belum diketahui. Penelitian ini dimaksudkan untuk mengetahui pengaruh kurkumin terhadap kadar cAMP dan kadar progesteron pada steroidogenesis kultur sel luteal dengan penambahan teofilin. Subyek penelitian adalah korpus luteum tikus Sprague Dawley yang diinduksi dengan PMSG. Kurkumin diberikan sesaat setelah stimulasi LH dan atau PGF2a dengan dan tanpa penambahan teofilin. Kemudian kultur sel diinkubasi selama 24 jam. Konsentrasi cAMP diukur dengan metode ELISA sedangkam konsentrasi progesteron diukur dengan metode RIA. Hasil penelitian menunjukkan bahwa LH meningkatkan cAMP dan kadar progesteron secara bermakna, sedangkan PGF2a mengurangi kadar cAMP dan kadar progesteron secara tidak bermakna. Teofilin meningkatkan kadar cAMP dan kadar progesteron secara bermakna dan hampir sama dengan stimulasi LH. Kurkumin menghambat kadar cAMP oleh LH maupun teofilin. Disimpulkan bahwa kurkumin menghambat kadar cAMP dan kadar progesteron pada kultur sel luteal dengan cara menekan transduksi sinyal di up stream cAMP.
Efek Pentagamavunon-0 terhadap Konsentrasi cAMP dan Progesteron pada Kultur Sel Luteal yang mengandung Teofilin Purwaningsih, Endang; Kadarsih Soejono, Sri; Dasuki, Djaswadi; Meiyanto, Edy
Jurnal Kedokteran YARSI Vol 17, No 2 (2009): MEI - AGUSTUS 2009
Publisher : Lembaga Penelitian Universitas YARSI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33476/jky.v17i2.202

Abstract

Curcumin analog (Pentagamavunon-0/PGV-0) can inhibit steroidogenesis of luteal cell culture. Corpus luteum secretes progesterone by LH stimulation. The main transduction signal of luteal cells steroidogenesis is through the cAMP/PKA. The objective of this study was to know the effect of PGV-0 on cAMP and progesterone concentration of luteal cell culture containing theophylline. The subject was corpus luteum of rat Sprague Dawley strain induced with PMSG (10 IU). PGV-0 was given shortly after the stimulation of LH and or PGF2? with or without theophyline. The cell culture then put into the incubator for 24 hours. Concentration of cAMP was assessed by ELISA whereas the progesterone concentration was determined by RIA. The result showed that LH stimulation caused cAMP and progesterone increase significantly. The inhibition of PGF2? on cAMP and progesterone concentrations showed no significant difference compared to the control. Theophylline increased the cAMP and progesterone concentration significantly but not to LH stimulation. PGV-0 did not inhibit cAMP concentration but PGV-0 inhibited the progesterone concentration by LH stimulation. In conclusion, PGV-0 inhibits signal transduction of lutheal cell in down stream cAMP.
Co-Authors Djaswadi Dasuki Edy Meiyanto Endang Purwaningsih