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Jurnal Penelitian Pertanian Tanaman Pangan

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Published by Pusat Penelitian dan Pengembangan Tanaman Pangan

ISSN : 25415166 EISSN : 25415174 DOI : 10.21082

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Jurnal Penelitian Pertanian Tanaman Pangan merupakan publikasi yang memuat makalah ilmiah primer hasil penelitian tanaman pangan (padi dan palawija). Redaksi menerima makalah dari peneliti Puslitbang Tanaman Pangan, Balai Pengkajian Teknologi Pertanian (BPTP), maupun perguruan tinggi. Makalah yang dikirimkan hendaknya sudah mendapat persetujuan dari pimpinan instansi masing-masing. Ketentuan penulisan makalah untuk dapat dimuat di jurnal ini tertera dalam "Author Guidelines/Petunjuk bagi Penulis" di halaman terakhir Jurnal Penelitian Pertanian.

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Articles 9 Documents

Search results for , issue "Vol 33, No 2 (2014): Agustus 2014" : 9 Documents clear

Keragaman Virulensi dan Konstruksi Molekuler Virus Tungro pada Padi dari Daerah Endemis Praptana, R. Heru; Sumardiyono, Y. B.; Hartono, Sedyo; Trisyono, Y. Andi; Widiarta, I Nyoman
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Tungro is an important disease of rice, constraining to the rice production in Indonesia. Tungro is caused by the infection of two different viruses namely tungro bacilliform virus (RTBV) and tungro spherical virus (RTSV). Both viruses are only transmitted by green leafhoppers, especially Nephotettix virescens in a semipersistent manner. The variation of tungro viruses from different areas had been reported, and there is a specific relationship between resistance variety and tungro virus isolate. It is important therefore, to study the virulences and the genetic diversities of tungro viruses derived from the endemic areas in Indonesia. This study was aimed to identify the virulence and the molecular diversity of tungro viruses from endemic areas in Indonesia. Susceptible variety TN1 was used in the study. Surveys and collection of the infected plants and green leafhoppers were conducted in some tungro endemic areas, including: West Java, Central Java, Yogyakarta, Central Sulawesi, West Sulawesi, South Sulawesi, Bali and West Nusa Tenggara. Artificial virus transmission using test tube method was used in the virulence test. Green leafhoppers caught from the field were used as vector transmitters. The virulence of tungro viruses was determined based on diseases indexes (DI). Results showed that the virulence of tungro viruses varied among region in the endemic areas in Indonesia. The Central Java virus isolate was the most virulence; however, not all isolates from endemic areas in the island of Java were more virulent than those from outside of Java. The presence of RTBV and RTSV was detected in the infected TN1 plants. The existences of molecular diversities of tungro viruses from the endemic areas were observed. The relationship between combination of DNA bands of RTBV and RTSV with the virulence in endemic areas outside of Java was more complex than it was in West Java and Central Java. The molecular diversities of tungro viruses were not correlated with the geographic difference of the endemic areas, nor with the virulences.

Pendugaan Parameter Genetik dan Seleksi Karakter Morfofisiologi Galur Jagung Introduksi di Lingkungan Tanah Masam Lubis, Khairunnisa; Sutjahjo, Surjono Hadi; Syukur, Muhamad; Trikoesoemaningtyas, Trikoesoemaningtyas
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Morpho-physiological characterization of sixteen inbred lines of maize originated from CIMMYT and from Indonesian Cereals Research Institute Maros, was carried out in Tenjo, Bogor, from January to April 2013. The objectives of this research were to (1) estimate the genetic parameters of morphophysiological characters, (2) obtain morpho-physiological characters that can be used as selection characters and (3) obtain tolerance inbred lines on acid soil condition. The experiment was conducted using a Nested Design with three replications, nested in selection environment. The first factor was environments, the second factor was genotypes. The two environments were acid soil with eAl 0.2 me/100 g (represented the optimum soil) and acid soil with eAl 1.87 me/100 g (represented the medium acid soil). The results showed that environment had significant effect on inbred lines performances. Characters of anthesis silk interval, number of seeds/cob, number of rows/cob, cob dry weight, seed dry weight and cob weight/plot could be used as selection characters for tolerance to medium acid soil condition; the inbred lines tested indicated high genetic variabilities and showed high heritability under such condition. Inbred line CLA 84, CLA 46, CLA 95 and 1042-13 each was found tolerant genotype on medium acid soil condition

Dampak Rehabilitasi Jaringan Irigasi Perdesaan terhadap Adopsi Teknologi Budi Daya Padi Zakaria, Amar K.
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

The rehabilitation of irrigation canal on the rural area is very important to rice farmers because the use of water is becoming more eficient and rice production and farmerâ€™s income is increasing. Increasing the rice productivity would be considered as an indication of farmerâ€™s participation on the adoption of technology. The present study was aimed to evaluate the technology adoption on rice farming in relation with the irrigation canal rehabilitation. The study was carried out in Majalengka district of West Java, during 2012 using survey method. The data were collected through the interview techniques to 44 farmer respondents using structured questionnaires. The data were cross tabulated and to measure the advantage of adopting the technology, Gross R/C ratio was calculated along with the break even point/BEP. Result of the analyses showed that after rehabilitation of the rural irrigation infrastructure the water supply was becoming more available and farmers were motivated to adopt the rice farming technology and the management of rice cultivation. The degree of technology adoption was considered as optimum and economically was feasible, as indicated by the value of R/C between 1.98 and 2.15, with the profitability of 49.6 percent to 53.5 percent. The partial budgetting analysis showed the B/C marginal of 2.59 and growing Ciherang rice variety is considered to be profitable.

Efektivitas Trichoderma sp. dan Gliocladium sp. sebagai Agen Biokontrol Hayati Penyakit Busuk Pelepah Daun pada Jagung Soenartiningsih, Soenartiningsih; Djaenuddin, Nurasiah; Saenong, M. Sujak
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Sheath blight is an important disease in corn. The disease could cause significant yield loss when infection occurs on susceptible varieties. Disease control using the microorganism antagonist is an alternative for disease management. Research was carried out in a laboratory, greenhouse and field from 2010 to 2012. The research objective was to compare several biological agents for controlling sheath blight disease on corn. In vitro laboratory tests identified that out of sixteen isolates of microorganisms, only 3 isolates which had the potency to suppress the pathogen of sheath blight over 50%, namely TT1; TM; and GM. Conidia development among the three isolates of microorganism the highest was by TT1. In the greenhouse, three isolates of microorganisms showed potential of decreasing sheath blight disease up to 70%. The Gliocladium isolates decreases the disease by 53%. Research results from the field indicated the antagonist had decreased sheath blight disease by 67%. Isolates of Trichoderma and Gliocladium fungus could reduce the yield loss by 23% by suppressing the infection of sheath blight disease.

Similarity of 26 New Released Rice Varieties and Rice Parental Hybrids Based on 36 SSR Markers Susanto, Untung; Satoto, Satoto; Rohmah, Nofi A.; Mejaya, Made Jana
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

More than 200 rice varieties had been released in Indonesia, but the genetic variability among those released varieties was suspected to be relatively low. Molecular markers, especially SSR could be used as a tool to disect the distinctness among rice genotypes, albeit phenotipically similar varieties. The technique could also be used to prove the authenticity of a variety. This research was aimed to obtain DNA fingerprinting data of new released rice varieties and hybrid parental lines using SSR markers. A total of 26 rice genotypes consisted of three upland, ten irrigated, five swampy rice varieties, along with eight hybrid parental lines were used in this experiments. The DNA was extracted from young leaf samples using CTAB modified method and was amplified with 36 SSR markers linked to important rice traits which spread accross the 12 rice chromosomes. The experiment was conducted in Plant Breeding Laboratory of Indonesian Center for Rice Research (ICRR) during 2012. The results showed that PIC value of the genotypes were mostly at medium level of the genetic diversity with the average value of 0.4451. The phylogenetic analysis showed that at the genetic distance of 10%, the genotypes were separated into 9 groups, i.e. Inpago 6, Inpara 5, and BH33d each stood alone while (Inpara 1, Inpara 2 , and Inpara 3); (Inpari 18 and Inpari 19, Inpago 4, Inpago 5, and Inpara 4); (Inpari 11, Inpari 12, Inpari 13, Inpari 14, Inpari 15, Inpari 16, Inpari 17, and Inpari 20); (GMJ6B, B6, and IR79156B); (PK21, BH95E, Bio9, and R14) each belong to one group. The grouping of the genotypes in this study seemed to follow the adaptation type to agro ecosystems. The hybrid parental lines tended to stay in different group from the inbred varieties. The application of these 36 SSR markers was able to distinguish among 26 genotypes rather distinctly.The use of more markers should give more powerful data to distinguish among genotypes.

Morfologi Bunga dan Korelasinya terhadap Kemampuan Menyerbuk Silang Galur Mandul Jantan Padi Rumanti, Indrastuti A.; Purwoko, B. S.; Dewi, Iswari S.; Aswidinnoor, Hajrial; Satoto, Satoto
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Good male sterile lines should have high and stable sterility, as well as have good characters of flowers that enhances the outcrossing ability. Research was conducted at Sukamandi field station during DS of 2010 to study the character and flowering behaviour and their genetic variability among the new CMS lines. Five new CMS lines were tested in the field in three replicates of randomized complete block design. The results showed that CMS derived from the Wild Abortive, Kalinga and Gambiaca all were early flowering. The new CMS lines have better flower characters than that of IR58025A, including the following: bigger stigma, higher stigma exsertion percentage, wider degree of glume opening, and longer duration of glume opening. Better flowering behaviour enhanced the ability of outcrossing, and resulted in seed set obtained from CMS lines, up to 25.90%; the traditional IR58025A CMS line produced seed set of only 2.98%. The significant positive correlation was found between seed set with stigma width (r = 0.44*), with stigma exsertion (r = 0.54*); and with degree of glume opening of male sterile lines (r = 0.42*); also with filament length (r = 0.47*) and degree of glume opening of maintainer line (r = 0.57**). Those characters are important for the seed set of CMS lines in the hybrid seed production.

Emisi Gas Rumah Kaca dan Hasil Gabah dari Tiga Varietas Padi pada Lahan Sawah Tadah Hujan Bersurjan Mulyadi, Mulyadi; Wihardjaka, A.
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

The alternating dry and flooded bedding system (Surjan) optimizes land availability, by integrating food crop culture in the lower bed and annual crops in the upper bed position, of the rainfed rice field. Rainfed rice productivity is generally low, to increase its productivity rice culture should integrate the management of crop, water and nutrients. Rice field is regarded as greenhouse gas sources, especially methane (CH4) and carbon dioxide (CO2). Field experiment was conducted in the rainfed rice field in Pati, Central Java, to determine GHGs emission and grain yield from three rice varieties, applied with cattle manure, planted in the rainfed rice field, using alternating beds system. The experiment was arranged in a randomized block design with three replicates and six treatments of the combination of cattle manure application and rice varieties. The rates of cattle manure were 5 and 30 t/ha, while rice varieties were Inpari 1, Inpari 6, Ciherang. Data observed included grain yield, soil pH, fluxes of CO2 and CH4. Combination of Inpari 6 with 5 t/ha cattle manure emitted GHGs higher than combination of Inpari 6 with 30 t/ha cattle manure, while GHGs emission from combination of Ciherang with 5 t/ha cattle manure was lower than combination of Ciherang with 30 t/ha cattle manure. Variety Inpari 1 emitted the lowest GHGs, both from the combination of 5 t/ha and of 30 t/ha. The average grain yield of Inpari 1, Inpari 6, and Ciherang in the rainfed lowland rice each was 6.27, 6.01, and 5.70 t/ha, respectively. The GHGs releases from the rice variety roots depend on the availability of organic matter in the soil that is used as energy source for GHGs forming microbes.

Kendali Ketahanan Genetik Padi terhadap Penyakit Tungro Muliadi, Ahmad; Nasrullah, Nasrullah; Sumardiyono, Y. B.; Trisyono, Y. Andi
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Genetic study of resistant to rice tungro disease was carried out in Indonesian Agricultural Biotechnology and Plant Genetic Resources Institute, Bogor. TN1 rice variety was used as susceptible parent and OBSTG02-124 line as resistant parent. Cross combinations between resistant vs susceptible parents obtained 6 crosses. The study materials consisted of 55 plants of susceptible parent (P1) and 35 plants of resistant parents (P2), 70 F1 plants, 70 F1R plants, 100 BC1-1 plants, 100-BC1-2 plants, and 300 F2 plants. The probable maternal effect was identified by comparing resistant to tungro disease of F1 plants vs. the F1 of their reciprocal crosses, using the t-test. Each population was planted in pot containing 5 kg of soil. Plants were inoculated at 7-10 days old with virus tungro isolate Subang, using 4-5 green leafhoppers for 5 hours. Visual symptoms were observed based on the Standard Evaluation System for Rice. ELISA test (non Precoated I-ELISA) was performed at 21 days after inoculation, using polyclonal antibodies RTSV (S) and the combined RTBV and RTSV (BS). The results showed that there was no maternal effect on the inheritance of tungro disease resistance. The resistant to tungro in OBSTG02-124 was controlled by two complementary recessive genes. The moderate heritability indicated that the environment play role in determining the degree of resistant to tungro desease.

Deteksi Cepat Viabilitas Benih Padi Menggunakan Gelombang Near Infrared dan Model Jaringan Saraf Tiruan Firdaus, Jonni; Hasbullah, Rokhani; Ahmad, Usman; Suhartanto, M. Rahmad
Jurnal Penelitian Pertanian Tanaman Pangan Vol 33, No 2 (2014): Agustus 2014
Publisher : Pusat Penelitian dan Pengembangan Tanaman Pangan

Viability is an important component of seed quality, which could be detained by germinating the seeds. Currently testing the seed viability of rice takes a long time (5-14 days), so it becomes a limiting factor in the seed production process. An alternative method for rapid seed viability detection is using the Near Infrared (NIR) spectra and using artificial neural network (ANN) as a data processing system. This research was aimed to study the use of NIR spectra and ANN to predict the viability of rice seeds. NIR reflectance (1,000-2,500 nm) of a Ciherang rice seed samples (40 grams), was used as the input data to develop the ANN model. A total of 60 samples were subjected to accelerated aging to obtain various levels of germination. The development of ANN models was done through calibration and validation of NIR spectra to the viability parameters. As ANN input, NIR reflectance of seed sample was given pretreatment data such as normalization, first derivative, second derivative, standard normal variate (SNV) and principal component analysis (PCA). The results showed that longer accelerated aging caused a decrease in seed viability. This was also indicated by the decrease in soluble protein and an increase in free fatty acids. The intensity of the NIR absorbance spectra also showed the same in the absorption region of soluble protein and free fatty acids. The best ANN models to predict the germination was 10PC-5-3 ANN with the SNV NIR reflectance used as the input data. Coefisien correlation of the validation was 0.8947, the value of ratio performance deviation was 2.2359 and the standard error performance was 9.9233%. The use of NIR spectra and ANN was potentially useful to perdict the viability of rice seeds more rapidly.

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