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INDONESIA
BIOEDUSCIENCE
Published by Universitas Muhammadiyah Prof. Dr. Hamka
ISSN : 2614154X     EISSN : 26141558     DOI : -
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education
BIOEDUSCIENCE is an open access journal that publishes research in the field of Biology and Biosain Education such as: Applied and implemented in education and learning, Botany, Zoology, Microbiology, Ecology, Biotechnology, Molecular Biology, Genetics, Bioinformatics, Cell and Developmental Biology, Biodiversity and Bioconservation. BIOEDUSCIENCE is published by Biology Education Study Program, Universitas Muhammadiyah Prof. Dr. Hamka, Indonesia regularly in June and December. ISSN : 2614-1558; P-ISSN : 2614-154X.
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Articles 14 Documents
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Search results for , issue "Vol 10 No 1 (2026): BIOEDUSCIENCE" : 14 Documents clear
Identification and Characterization of Pathogenic Fungi Causing Necrosis in Vigna unguiculata
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18508

Abstract

Background: Vigna unguiculata (cowpea) is an important legume crop whose productivity can be affected by foliar diseases, including leaf necrosis caused by fungal pathogens. Identification of fungi associated with necrotic symptoms is essential as baseline information for plant disease management. Methods: This study aimed to identify fungi associated with necrotic lesions on cowpea leaves. Leaf samples showing necrotic symptoms were collected from agricultural fields in Tengger Wetan Village, Kerek Subdistrict, Tuban Regency, East Java. Fungal isolation was performed from infected leaf tissues to obtain pure cultures. Identification was conducted based on macroscopic colony characteristics and microscopic features using slide culture techniques and standard morphological identification keys. Results: Two dominant fungal isolates were consistently associated with necrotic symptoms on cowpea leaves. These fungi were identified morphologically as Alternaria alternata (isolate D1) and Nigrospora sp. (isolate D3). A. alternata was characterized by yellowish-white colonies, hyaline septate hyphae, and dark oval conidia, whereas Nigrospora sp. exhibited cottony white colonies with brown, globose conidia. Both fungi were associated with brown to black necrotic spots on the leaf surface. Conclusions: The findings provide initial information on fungal species associated with leaf necrosis in cowpea based on morphological identification. This information is useful as a foundation for further pathological and molecular studies to confirm species identity and support the development of appropriate disease management strategies.
Potential of Some Natural Media as Alternative Media for Growth of Bacillus sp.
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18527

Abstract

Background: Bacillus sp. as a biological agent can be an environmentally friendly disease control solution by producing antimicrobial compounds. However, expensive culture media is an obstacle in its use. Alternative media made from natural materials such as legumes are considered potential because they are rich in nutrients and affordable. This study aims to find the best alternative media that support the growth and viability of Bacillus sp. isolate Bcz 30. Method: This research was conducted at the Plant Health Laboratory of the National Development University “Veteran” East Java in July-November 2024. This research used a completely randomized design (RAL) with one stage of in vitro testing. The implementation of the test stages includes testing the growth of Bacillus sp. isolate Bcz 30 on each alternative media, testing the viability of Bacillus sp. isolate Bcz 30 on liquid media then the two highest viability results on liquid media will be continued for viability tests on different formulations, namely solid and liquid formulas. Analysis of the data obtained using the variance analysis test (ANOVA), if the data presented is significantly different, it will be continued with the BNJ test at the 5% level. Result: Based on the growth test, tolo bean and lamtoro gung media can replace NA media. Based on the liquid formula viability test, tolo bean and lamtoro gung media with 30% concentration had the highest viability results. Based on the formulation test, the solid formula can maintain the viability of Bacillus sp. than the liquid formula. Conclusion: Bean and lamtoro gung media with 30% concentration formulated with solid formula is the best alternative media for Bacillus sp. isolate Bcz 30.
Design and Validation of Interactive Multimedia for Teaching The Human Transport System in Senior High School
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18758

Abstract

Background: Biology learning in high school requires multimedia to support the learning process. One of the multimedia developed is BioTranspezia. BioTranspezia is a technology-based media. Existing multimedia on the human transport system is generally static, less interactive, and not fully adapted to high school learning needs, so the development of innovative multimedia is still needed. The purpose of this study is to develop BioTranspezia multimedia as a biology learning medium for the human transport system. Methods: The research used a development research approach based on the Waterfall Process Model, with the stages of Communication, Planning, Modeling, Construction, and Deployment. This study focuses on assessing the feasibility of the developed multimedia and the perceptions of users, namely 2 teachers and 20 students (limited trial) and 36 (field trial). Results: BioTranspezia multimedia, validated by media experts, gets a score of 86% with the criterion of "very feasible"; validated by material experts, it gets a score of 84% with the criterion of "very feasible". The user (teacher) implementation obtained a score of 99% with the criteria of "very good"; the student response obtained a score of 81.24% with the criteria of "very good". Conclusions: BioTranspezia Multimedia on the human transport system material is very feasible and highly suitable for use in the Biology learning process.
Exploring the Pharmacological Potential of Centella asiatica: A Metabolomic Perspective on a Traditional Medicinal Plant Aini, Fadita Nurul; Yuwana, Cynthia Putri; Maryenti, Tety; Nisa, Upi Chairun; Yuniati, Ratna; Handayani, Windri; Yasman, Yasman
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18857

Abstract

Background: Centella asiatica, also known as Gotu Kola or Pennywort, is a medicinal herb with a long history of use in traditional systems such as Mediterranean Herbal Medicine, Ayurveda, and Traditional Chinese Medicine. Its longstanding use highlights its importance as a natural remedy for various health conditions.   Methods: Recent metabolomics-based studies have advanced our understanding of its pharmacological potential by identifying key bioactive compounds—including phenolics, triterpenoids, and saponins—through analytical techniques such as GC-MS, LC-MS, and NMR.   Results: These compounds contribute to a range of therapeutic effects, notably antioxidant and antibacterial activities. C. asiatica has demonstrated efficacy against antibiotic-resistant pathogens like Staphylococcus aureus and Pseudomonas aeruginosa, as well as the ability to mitigate oxidative stress.   Conclusions: The integration of pharmacological and metabolomics approaches has highlighted its potential for development into herbal medicines and health supplements. While further research is warranted to optimize formulation and clinical application, existing evidence provides a solid foundation for the advancement of C. asiatica as a natural product for promoting human health and addressing antimicrobial resistance.
Pengaruh Cahaya dan Wadah Penyimpanan terhadap Efektivitas Nuclear Polyhedrosis Virus dalam Mengendalikan Spodoptera litura
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/20321

Abstract

Spodoptera litura pest attacks can cause disturbances in plant growth and lead to considerable yield losses. Control that is often done to control S. litura is usually with chemical insecticides, but excessive use of chemical insecticides has the potential to cause adverse effects on the environment. As a safer and more sustainable alternative, pest control can be done through the utilization of biological agents such as pathogens that function as bioinsecticides, one of which is Nuclear Polyhedrosis Virus (NPV). This research aims to analyze the effect of length of exposure to sunlight and the type of storage container on the effectiveness of NPV in controlling S. litura. NPV isolates were exposed to direct sunlight for 1, 3, 6 hours in glass and plastic vial containers. The isolate was infected to 3rd instar S. litura larvae using feed contamination technique. This research implemented a factorial Completely Randomized Design (CRD), with 6 treatments and control. The results showed that NPV isolates with MK.1 and MP.1 treatments were effective in producing a percentage of larvae mortality and were able to suppress the formation of pupae and imago with a mortality percentage of 84,2 % and 71,4 % at 72 hours after aplication. Length of sunlight exposure affects the effectiveness of NPV isolates.
Formulation and Physicochemical Characterization of a Macroemulsion Containing Ethanolic Extract of Kemuning Leaves (Murraya paniculata (L.) Jack)
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/20395

Abstract

Background: Ethanolic extract of Murraya paniculata leaves contains several bioactive constituents, but its direct application is limited by physicochemical instability and solubility issues. Objective: This study aimed to formulate and characterize macroemulsions containing ethanolic extract of Murraya paniculata leaves and to identify the most promising formulation based on physicochemical evaluation.   Methods: The extract was prepared by maceration using 96% ethanol. Four macroemulsion formulations were developed using different ratios of virgin coconut oil, Tween 80, PEG 400, and water. The formulations were evaluated through phytochemical screening, organoleptic properties, emulsion type, transmittance, particle size distribution, pH, zeta potential, centrifugation, and cycling test.   Results: The extract contained flavonoids, alkaloids, tannins/phenolics, and triterpenoids. All formulations were identified as oil-in-water emulsions. Based on the reported data, F2 showed high transmittance and a low polydispersity index, while F1 and F2 demonstrated better physical stability than F3 and F4.   Conclusions: The study indicates that F1 and F2 were the most promising formulations; however, the claim of a single best formulation should be supported by complete, consistently presented particle-size, zeta-potential, and stability data.
Effect of Local Microorganisms from Calamansi Peel and Banana Peel Addition on the Quality of Liquid Organic Fertilizer (POC) Produced from Tofu Wastewater
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/21024

Abstract

Background: Bengkulu Province has seen a significant rise in calamansi orange (Citrus microcarpa) cultivation since 2010. While popular for their unique aroma and high flavonoid content (including ascorbic and citric acids), they also generate significant byproduct potential. To optimize the quality of Liquid Organic Fertilizer (POC) derived from this waste, specific additives are required to ensure the final product meets established quality standards. Tofu wastewater is an organic by-product with potential for use as liquid organic fertilizer. This study aimed to determine the effect of adding local microorganisms derived from calamansi peel and banana peel on the nutrient quality of liquid organic fertilizer produced from tofu wastewater. Methods: The measured parameters were nitrogen (N), calcium (Ca), phosphorus (P), and potassium (K). This study used an experimental design with four treatment groups: control, 1 kg, 2 kg, and 3 kg additions of fruit peel-based materials. Data were analyzed using one-way ANOVA. Results: The highest values of N, Ca, P, and K were observed in the 3 kg treatment, at 0.56%, 0.18%, 0.42%, and 0.42%, respectively. Statistical analysis showed significant differences in N, Ca, and K contents among treatments, whereas P content did not differ significantly. Conclusions: These findings indicate that the addition of calamansi peel and banana peel materials influenced several nutrient parameters of the liquid organic fertilizer, although not all parameters met the expected quality standard
Effect of EM-4 Concentration on the Decomposition Process of Compost Made from HVS Paper Waste
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/21261

Abstract

Background: In accordance with the Regulation of the Minister of Environment and Forestry of the Republic of Indonesia Number 6 of 2022 concerning the National Waste Management Information System, waste management activities at each facility may include processing waste into compost. Paper waste accounts for approximately 10% of total waste generation in Indonesia. Given that paper fibers can only be recycled five to seven times before losing structural integrity, composting serves as a vital alternative for waste reduction. Paper waste is one of the major types of solid waste generated in educational institutions and offices. One alternative for its utilization is composting with the addition of effective microorganisms (EM-4) to accelerate decomposition. This study aimed to determine the effect of different EM-4 concentrations on the composting process of HVS paper waste. Methods: Paper waste is one of the major types of solid waste generated in educational institutions and offices. One alternative for its utilization is composting with the addition of effective microorganisms (EM-4) to accelerate decomposition. This study aimed to determine the effect of different EM-4 concentrations on the composting process of HVS paper waste. Results: EM-4 concentrations of 30 mL, 35 mL, and 40 mL produced better compost characteristics, including lower final temperatures closer to ambient conditions, darker color, finer texture, and faster decomposition times than the 20 mL, 25 mL, and control treatments. Conclusions: EM-4 concentrations of 30–40 mL were more effective for composting HVS paper waste under the conditions of this study.
Characteristics of Solid Soap from Used Cooking Oil and Kalamansi Orange Peel Essential Oil
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/21564

Abstract

Background: Used cooking oil is defined as cooking oil that has been used for frying more than three times. Consuming used cooking oil poses serious health risks because it can cause several diseases and its waste can damage the environment. The purpose of this study was to determine the characteristics of foam height, pH, and organoleptic tests on solid soap made from used cooking oil with calamansi orange peel essential oil. Methods: This study was quasi-experimental using one liter of used cooking oil as a sample. Analysis was carried out univariately. Results: Based on the research results, the color, aroma, and shape of solid soap made from used cooking oil passed the organoleptic test and met the requirements of SNI 06 3532 1994. The average pH test results showed a pH value of 9, which met the SNI 06 3532 1994 standard. The results of the test on the foam height of solid soap from used cooking oil showed an average foam height of 5.91, which did not meet the SNI 06 3532 1994 standard. Conclusions: The results of this study aim to provide information to the public about the method of processing unused used cooking oil into more useful materials and increase economic value
Effect of Durian Peel and Young Coconut Shell Composition on the Quality of Biomass Briquettes as Alternative Fuel
BIOEDUSCIENCE Vol 10 No 1 (2026): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/21897

Abstract

Background: The use of fossil fuels can cause various impacts that lead to health problems. Therefore, an alternative energy source is needed to reduce environmental impact. One form of biomass energy is briquette production. Briquette materials can be obtained from industrial waste, namely durian peels and young coconut shells. Methods: This study employed a laboratory-scale experimental design to evaluate the effect of material composition on briquette quality parameters. Results: The results showed that variations in the composition of durian peel and young coconut shells produced different briquette qualities for each parameter, with a 2:1 mixture providing the most optimal quality with low moisture content (3.91%), low ash content (6.52%), high fixed carbon (85.04%), controlled volatile matter (4.53%), and high calorific value (6096 cal/g). The ANOVA test showed p-values < 0.05 for all quality parameters, indicating that variations in material composition significantly affect the quality of the briquettes produced. Conclusions: This research indicates that the quality of briquettes made from a mixture of durian peel and young coconut shells is influenced by composition, with a 2:1 ratio producing the best quality based on parameters such as moisture content, ash content, bound carbon, volatile matter, and calorific value. Further research is recommended using a wider range of ratios and additional testing, such as durability and combustion emissions, to strengthen the assessment of briquette quality.

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