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Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal

biosfera
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Published by Universitas Jenderal Soedirman

ISSN : 08531629 EISSN : 08531629 DOI : -

Core Subject : Science, Agriculture, Social,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

Majalah Ilmiah Biologi Biosfera : A Scientific Journal merupakan peer reviewed jurnal yang diterbitkan oleh Fakultas Biologi Universitas Jenderal Soedirman. Media ini mewadahi hasil-hasil penelitian di bidang biologi tropika yang terbit tiga kali setahun (Januari, Mei, September).

Arjuna Subject : -

Articles 7 Documents

Search results for , issue "Vol 26, No 3 (2009)" : 7 Documents clear

Komunitas Biota Hewan Bentik Pada Danau Paparan Banjir Di Kalimantan Timur Lukman Lukman; T Chrismadha; Muhammad Fakhrudin; J Sudarso
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Identifikasi dan Karakterisasi Isolat 23 Ducc Serta Kemampuannya dalam Memproduksi Enzim Inulinase Wijanarka Wijanarka; K Endang; Hermin Hermin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Inulinase has a capacity to convert inulin into fructose hence it could be used in high fructose syrup production. Inulinase is not readily available in Indonesian market. This enzyme could be obtained from thermotolerant yeast found in Dahlia variabilis. Therefore this study was conducted to identify inulinolytic yeast in D. variabilis. The results showed that an inulinolytic yeast isolate 23 DUCC from D. variabilis tubers was found. The inulinolitic yeast produces inulinase (E.C.3.2.1.7). Based on the identification and determination, the isolate is known to be Kluyveromyces marxianus. The best optimation condition for this yeast was as follows: concentration of carbon source (inulin) was 0.75%, pH was 5.5 and time of fermentation was 53 hours. In such condition K. marxianus produced inulinase up to 0.6481 IU.

Peningkatan Kualitas Akuakultur Menggunakan Teknologi Biofilter Mikroalga Imobil Rini Riffiani
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Perancangan Primer Oligonukleotida untuk Polimerisasi in Vitro Gen Sukrosa Sintase Pieter Agustinus Riupassa
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

The most important problems in using polymerase chain reaction (PCR) are the efficiency of energy, cost and time due to gene amplification. Oligonucleotide primer design of sucrose synthase gene was conducted as a model of preliminary experiment to amplify gene using PCR. In plant cells, this gene plays an important role in carbohydrate metabolism, a sucrose molecule break down into glucose. This design involved some computer software as bioinformatics tools. Five data sequences of legumes were downloaded from gene bank using accession number of AF030231, AJ311496, X92378, X69773, and D10266 belongs to soybean, pea, alnus bean, fava bean, and mung bean, respectively. After sequences alignment, some conservative regions were determined as the basis to construct forward and reverse primer candidates. Furthermore, the candidates were tested for compatibility. The results showed that the oligonucleotide primers can amplify sucrose synthase gene with ± 1462 bp fragment size using 5’-AACTTTgTgCTTgA-3’ and 5’-TCCTTTgACTCCTTC-3’ for forward and reverse primer, respectively. Even the PCR process weren’t applied, those primers might be universal primers to amplify sucrose synthase gene of legume plants.

Isyarat Sentuhan Antena Semut (Dolichoderus Sp) Pada Tubuh Aphid (Aphis Sp) Untuk Memperoleh Embun Madu Yayan Sunjaya; Harry Buana; Mimi Halimah
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Ant, Dolichoderus sp, interact mutually with aphid (Aphis sp). The ants specifically communicate with the aphid by means of antennal sign. A hungry ant will approach an aphid and touch it dorsal part using ant antennae; in respond to this, will expel honey dew. To further study the pattern of communication between the ant and the aphid, their movement was recorded using Handycam JVC GR-SXM 300 AC. The records were processed using Adobe Premier Pro 1.5. The results showed that the ant antennae moved in a specific pattern which could be classified into 4 main steps. The first step consisted of 8 movements which formed 2 movement patterns. The second step consisted of 3 main repeated movements. The third step consisted of 3 main repeated movements. The fourth step consisted of 6 main movements which were repeated several times before the final movement. Such pattern of antennae movement was recognized by the aphid as tactile signal of ant identity.

The Dynamic Or Testicular Activity Of The Hard-Lipped Barb (Osteochilus Hasselti C.V.) Under Extended Photoperiod A Wulandari; Soeminto Soeminto; Gratiana E Wijayanti
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Hormonal manipulation has been widely used in aquaculture to promote fish reproduction. However some concern has been raised in accordance to it effect of human health as well as environmental issues. Therefore alternative approaches in promoting fish reproduction need to be continuously studied. Photoperiod manipulation offers a favorable alternative since it is one of the most crucial environmental cues necessary for fish reproduction. An experimental study has been conducted to evaluate the effect of various photoperiods on testicular activities in the hard-lipped barb (Osteochilus hasselti C.V.). Four groups of fish were reared under different photoperiod i.e. 14L:10D, 16L:8D, 18L:6D and the control group were reared under natural photoperiod for eight weeks period. Forth nightly three fish of each group were stripped to aspirate milt for measurement of milt volume and sperm concentration, they were decapitated for gonado-somatic index calculation and spermatogenic activities. The results showed that the gonado somatic index (GSI) of the control group, 14L:10D and 18L:6D increased throughout the experimental period, meanwhile GSI of the fish reared under 16L:8D increased up to 4th week then decreased until the end of the study. The fluctuation of the GSI values was followed by the fluctuation of milt volumes and sperm concentration. This can be concluded that rising daily photoperiod up to 16 hours could improve reproduction in male hard-lipped barb.

Konstruksi Mutan Pseudomonas sp. untuk Meningkatkan Produksi Indole Acetic Acid (IAA) melalui Mutagenesis dengan Transposon Aris Tri Wahyudi; Mutiha Panjaitan; Nisa Rachmania
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 26, No 3 (2009)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Pseudomonas sp. is one of bacterial groups having ability to promote plant growth and health. Of a hundred successfully isolated Pseudomonas sp. from soybean rhyzosphere, 98 were found to produce indole acetic acid (IAA) ranging from 0.33 to 16.02 ppm. These isolates are bacilli, motile, Gram negative, and showing positive oxidase assay. One of them, i.e. Pseudomonas sp. CRB17, can promote plant growth by means of significant stimulation of primary root length and lateral root number. This isolate was then subject to mutagenesis using transposon Mini-Tn5Km1 to increase IAA production. Mutagenesis was done by conjugation between E coli S17-1 (l pir) carrying transposon mini-Tn5Km1 (donor) and Pseudomonas sp. (recipient), resulting in conjugation frequency of approximately 3.1 x 10-5 cell per recipient. The resulted CRB17 mutants were then tested for their ability to produce IAA, one of which showed an increment of IAA production up to 77.5%, while some others showed no significant change or even had a reduction to 55.3%. Sequence analyisis of 16S rRNA gene of Pseudomonas sp. CRB17 indicated that it has a high homology with that of Pseudomonas plecoglossicida (identical value of 99%). The results recommends that mutagenesis using transposon can be applied to increase IAA production, especially in Pseudomonas sp. CRB17.

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