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INDONESIA
Jurnal Perikanan Universitas Gadjah Mada
Published by Universitas Gadjah Mada
ISSN : 08536384     EISSN : 25025066     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Jurnal Perikanan Universitas Gadjah Mada are published to promote a critical review of the various investigative issues of interest in the field of fisheries between the researchers, academics, students and the general public, as a medium for communication, dissemination, and utilization of wider scientific activities.
Arjuna Subject : -
Articles 19 Documents
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Search results for , issue "Vol 8, No 1 (2006)" : 19 Documents clear
Feminisasi Nila (GIFT), Oreochromis sp. Menggunakan Hormon Estradiol 17-β Titin Kurniasih; Otong Zenal Arifin; Marizal Marizal
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.166

Abstract

Research to find out the effect of oral administration of estradiol 17-β  on feminization of Oreochromis sp. (GIFT) fry was carried out at Cibalagung Research Station. Five different concentrations of estradiol 17-β  i.e.: 60, 80, 100, 120 and 0 mg/kg feed, were given to larvae of 6 days old twice a day for 21 days in triplicates. The result indicated that there was significant difference of average percentage of female between control and treatments.The highest percentage of female was obtained from fish treated with 100 mg estradiol /kg feed (86.6%), whereas the lowest was obtained from fish control (51.7%). The survival rate and length growth were not significantly different. Survival rate of larvae ranged from 94 to 97.7% and the average of length growth ranged from 10.08 to 13.32 mm.
Metode Loop-Mediated Isothermal Amplification (LAMP) dan Aplikasinya untuk Deteksi Penyakit Ikan Murwantoko Murwantoko
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.156

Abstract

Polymerase chain reaction (PCR) is a method that amplifies DNA which have been widely used in molecular biology technique. Based on the PCR, many methods have been developed on isothermal condition and the useful one is loop-mediated isothermal amplification of DNA (LAMP). LAMP reaction employs a Bst DNA polymerase and a set of four specific primers that recognizes a total of six distinct sequences of the target DNA and produces amount of different size of DNA. Many advantages have been achieved in LAMP such as the simple equipment for reaction and observation, short time, highly specific and sensitive procedure. LAMP has been used as a tools for detection many pathogens for human, animals and plants. Some fish pathogens as parasites, bacteria and viruses have been detected by LAMP. The principles and application of LAMP method are discussed in this paper.
TINGKAT KERUSAKAN SEL DARAH MERAH LELE DUMBO (Clarias gariepinus) YANG DIPAPARKAN DALAM ENDOSULFAN PADA KONSENTRASI SUBLETAL Indra Gumay Yudha
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9027

Abstract

The objective of this experiment was to study the effect of endosulfan on red blood cells of catfish, C. gariepinus. Ten fishes, (2-3 g of body weight), were kept in 30 l fish tank. Fish were exposed to 5 concentrations of endosulfan (1.71 ppb, 3.43 ppb, 5.14 ppb, 6.85 ppb and 8.57 ppb) for 6 weeks. The experiment was done in triplicates. The result showed that endosulfan caused the red blood cells damage by forming cerroids and lipofuscin complex. This impairment was over 90% of total red blood cells when fishes were exposured to endosulfan at 6.85-8.57 ppb.
Aspek Biologi dan Potensi Lestari Sumberdaya Lobster (Panulirus spp.) di Perairan Pantai Kecamatan Ayah Kabupaten Kebumen Muammar Kadafi; Retno Widaningroem; Soeparno Soeparno
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.171

Abstract

The research on biological aspects and maximum sustainable yield of spiny lobster (Panulirus spp.) in Ayah coastal waters Kebumen Regency aimed to know biological aspects (including species of lobster, length-weight relationships, and sex-ratio), maximum sustainable yield, and exploitation rate of spiny lobster. Biological data were collected in January-February 2004 with census and survey methods. Production and effort data between 1998 to 2003 were used to estimate maximum sustainable yield of spiny lobster. This research found six species of lobsters (Panulirus homarus, P. ornatus, P. penicillatus, P. longipes, P. versicolor, and P. polyphagus). Length-weight relationships based on the sex and carapace length showed isometric and allometric growth models. Male and female ratio was 1,06:1. The maximum sustainable yield was 19,498 kg/year and the exploitation in this area was already overfished.
Distribusi Residu Logam Berat Timbal (Pb) dalam Organ Ikan Bandeng (Chanos chanos) PADA Salinitas Air Berbeda Petrus Rani Pong-Masak; Rachmansyah Rachmansyah
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.162

Abstract

Determination of plumbum (Pb) residue distribution in the organs of milkfish, Chanos chanos reared in different water salinities was performed in a 2,000 l fiberglass container under laboratory condition. Water salinity levels as treatment were 10, 20, and 30 ppt. Milkfishes (100±14 g) obtained from local farmer were reared in 2,000 l fiberglass container at density of 40 individu/l. The fishes were exposured with Pb at 2 ppm. Sampling were conducted at 0 (initial), 7, 14 and 21 days after application of Pb. Pb was extracted from flesh, kidney, liver, intestine, and gill, and determined by atomic absorption spectrophotometer. Results showed that Pb residue in milkfish organs increase when water salinity decrease. The highest Pb residue concentration found in kidney followed by liver, intestine, flesh and gill.
Evaluasi Laju Pertumbuhan, Keragaman Genetik Dan Estimasi Heterosis pada Persilangan Antar Spesies Ikan Patin (Pangasius sp.) Didik Ariyanto; Retna Utami
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.167

Abstract

Thai catfish (Pangasius sp.) is one of the most popular cultured catfish in Indonesia. There are 2 species Thai catfish i.e. P. hypopthalmus which introduced from Thailand in 1972 and P. djambal an endogenous Indonesian fish. The aims of this research were to evaluate the growth rate, genetic variability and heterosis estimacy on interspecific crossing of these species. The result showed that the growth rate of F1 P. hypopthalmus X P. djambal was better than its parents. Genetic variability of P. hypopthalmus, P. djambal and their hybrids were 12.54, 12.38, and 27.02, respectively, and heterosis estimacy of their hybrids on growth (length and weight) were in high categories about 48.98-257.90%.
APLIKASI MINYAK CENGKEH SEBAGAI OBAT BIUS PADA KAKAP PUTIH (Lates calcarifer Bloch) Bambang Hanggono
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.157

Abstract

The objective of this study was to determine the safety and efficacy of clove oil as an anesthetic in sea bass (Lates calcarifer) and the potential application of clove oil as anesthetic to facilitate the sea bass fry transportation. Acute toxicity test indicated the 24-hr LC50 value of clove oil in sea bass fry as 30 ppm with slope function of 1.079 (1.05 to 1.107). In efficacy test, fish were exposed to 5, 10, 15, 20 and 25 ppm of clove oil for 15 minutes. At 5 ppm, clove oil caused only sedation effect (partial loss of reaction to external stimuli) while at 20 ppm, fish entered anesthesia stage (failure to respond to external stimuli) within about 3 minutes. Fish recovered from a 15-min period of exposure in 20 ppm clove oil within less than 10 minutes following removal from the anesthetic solution. There was neither mortality nor abnormal behavior of fish during 15-min exposure of clove oil as well as during 7 days post recovery from anesthesia. The potential application of clove oil as an aid in the transport of sea bass fry in plastic bag was also investigated. At 5 ppm, clove oil could reduce activities of the fish without loss of equilibrium (sedation stage) during the 4 hour simulated transport at 50 fish per 1,000 ml sea water (15 ppt). At 20 ppm, clove oil caused loss of equilibrium in fish resulting in the anesthesia stage throughout the 4 hour period. However, there was no improvement on survival rate and fish behavior with the use of clove oil during and after this 4 hour transport. Simulated transport at 50 fish per 500 ml sea water (15 ppt) for 8 hour did show better significant survival rate with additional of 5 and 20 ppm clove oil. In both short and long term transport study, clove oil did show the benefit by reducing the fish activities judging from the reduction of oxygen consumption, ammonia and carbon dioxide levels. Addition of appropriate concentration of clove oil in transport water ensured that the fish would stay calm by reducing fish activity and therefore, prevented any drastic changes of water qualities.
STRUKTUR KOMUNITAS TERIPANG (HOLOTHUROIDEA) DI PERAIRAN TANJUNG MERAH, SELAT LEMBEH SULAWESI UTARA Eddy Yusron; Susetiono Susetiono
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.9028

Abstract

During January 2005 the holothurian community inhabiting littoral areas was assessed at five station on four ecological indices: density, species richness, diversity and evenness. It was revealed that the holothurian community consisted of 8 species in which three among them occurred in all locations at higher densities: Holothuria edulis, H. scabra and H. atra. Small variations of the ecological indices found in all location might indicated that the community characteristics among locations were relatively similar.
Pengaruh Perbedaan Jenis Pakan terhadap Pertumbuhan Populasi Brachionus plicatilis Regina Melianawati; Adi Hanafi; Made Suastika
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.172

Abstract

The objective of this research was to know the effect of different feed on the growth of rotifer, Brachionus plicatilis population. Three combinations of feed namely (A) yeast + enrichment ingredient; (B) Isochrysis tahiti + yeast + enrichment ingredient and (C) Isochrysis tahiti were used as treatments in triplicates. Initial density of B. plicatilis was 100 ind./ml. Feeding frequency was twice per day, at 08:00 am and 16:00 pm. This experiment was carried out for 72 hours, and sampling was done every 24 hours. The results showed that the highest density for treatment A, B and C were 330.3±7.6, 305.0±63.8, and 300.0±10.0 ind./ml, respectively. In addition, the highest population reached at 660,667, 610,000 and 600,000 individuals for treatments A, B, and C, respectively. Percentages of B. plicatilis with eggs in each population were 15.14, 20.46, and 13.95% for treatments A, B, and C, respectively. These results indicated that yeast and enrichment ingredient gave the highest growth of Brachionus plicatilis population.
Polimorfisme Enzim Glucose-6-Phosphate Isomerase pada Tiga Populasi Tuna Sirip Kuning (Thunnus albacares) Gusti Ngurah Permana; Jhon H. Hutapea; Sari Budi Moria; Haryanti Haryanti
Jurnal Perikanan Universitas Gadjah Mada Vol 8, No 1 (2006)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.163

Abstract

Samples of yellowfin tuna (Thunnus albacares) were taken from three locations Bali, North Sulawesi and North Maluku. The glucose-6-phosphate isomerase (GPI) was analyzed from liver using allozyme electrophoresis method. Polymorphism of GPI enzyme was observed and four alleles (A, B ,C, D) were found in Bali population, three alleles (A,B,C) were found in North Maluku and North Sulawesi populations. Heterozygosity values, from Bali, North Maluku and North Sulawesi were 0.419; 0.417; 0.143 respectively. Genetic distance between North Sulawesi and North Maluku were 0.029, and with Bali population was 0.353. These results indicated that North Maluku and North Sulawesi population were not separate by geographic barrier, therefore genetic distance of both populations was closed. However, those populations were genetically separated to the Bali population might be due to major hydrological barrier.

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